FastQCFastQC Report
Fri 10 Feb 2017
SRR2031574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907237
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43350.2272921508968209No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30550.16017935893651392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.10108864289021238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.056.7391321
GTATCAA44450.045.7367821
TCAACGC52350.038.1088834
ATCAACG52500.038.0904773
CAACGCA53500.037.289725
AACGCAG54750.036.5251166
TATCAAC56500.035.5619472
ACGCAGA61200.032.675657
CGCAGAG61950.032.1267138
GTACATG47750.026.9581171
GCAGAGT70850.026.9513059
TACATGG47850.025.7105542
ACATGGG47250.024.9312173
AGAGTAC62700.023.03030210-11
GAGTACT44500.022.52247212-13
CAGAGTA68400.021.87510-11
CATGGGG29100.019.750864
AGTACTT52000.019.54807912-13
GTACTTT50850.019.19616514-15
TGGACCG1650.001464291217.2727285
Produced by FastQC (version 0.11.5)