FastQCFastQC Report
Fri 10 Feb 2017
SRR2031574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907237
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.2802483383030006No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT44360.23258776963743888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.20390753744815143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.1273045772497073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.055.338071
GTATCAA51750.045.956361
ATCAACG60650.039.3147053
TCAACGC60800.039.2177124
AACGCAG62200.038.564096
CAACGCA62300.038.3497055
TATCAAC65150.036.7449952
ACGCAGA70850.033.721767
CGCAGAG72350.032.825678
GCAGAGT84400.027.4074489
TTATGCG1000.001654299223.749374
GTACATG48550.023.6109681
TACATGG48300.023.4051762
AGAGTAC73150.023.31107110-11
ACATGGG46300.023.185133
CAGAGTA79500.022.61418210-11
CATGGGG24300.022.4788284
GAGTACT55350.022.4406912-13
GTATACG1353.7623968E-421.1399251
GTATATA14650.021.103851
Produced by FastQC (version 0.11.5)