FastQCFastQC Report
Fri 10 Feb 2017
SRR2031573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259993
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.1672231512397291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.13134993607107343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.049.6525651
GTATCAA30650.043.2381741
TCAACGC34900.037.972784
CAACGCA36250.036.820695
ATCAACG36100.036.578953
AACGCAG36700.036.369216
TATCAAC38000.034.8752
ACGCAGA41400.032.1256037
CGCAGAG41450.031.9722588
GCAGAGT46750.027.4331539
GTACATG40200.024.4589561
TACATGG39400.024.3527932
ACATGGG37550.024.034623
AGAGTAC43450.022.35615710-11
CAGAGTA45300.022.01986710-11
ACTACGC1100.002877518521.590918
GAGTACT26900.021.54275112-13
CCATGCG1656.3233354E-520.1515169
TCGCGCT600.004765046319.79166870-71
AGTACTT29300.019.2918112-13
Produced by FastQC (version 0.11.5)