FastQCFastQC Report
Fri 10 Feb 2017
SRR2031573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259993
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT35310.2802396521250515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26090.2070646424226166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.1600802544141118No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT15560.1234927495628944RNA PCR Primer, Index 26 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.052.1638071
GTATCAA29100.042.9763371
TCAACGC33500.036.864844
CAACGCA34500.035.9339755
ATCAACG35350.034.9355623
TATCAAC36350.034.4971582
AACGCAG36250.034.4612926
ACGCAGA41800.029.8856897
CGCAGAG42800.028.9654678
TACATGG34400.024.992172
GCAGAGT48900.024.8665149
ACATGGG32500.024.5532913
GTACATG36650.023.6137031
CATGGGG21050.022.1135144
GTATAAT10200.021.4449141
AGAGTAC44000.021.42849310-11
GAGTACT31000.020.30196212-13
CAGAGTA46000.020.13541410-11
AGTACTT34200.018.68013412-13
TACGCTC1300.007564503718.268824
Produced by FastQC (version 0.11.5)