FastQCFastQC Report
Fri 10 Feb 2017
SRR2031572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331843
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA50251.5142703025225783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42921.2933827141148073No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT39851.2008690856820845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37121.118601266261455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29150.878427449125038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22370.6741139635309469No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.6192687505838604No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT14760.44478865005439316No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC14320.4315293678034492No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA8170.2462007636141187No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG6900.20792965348071227No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC5880.17719222644443305No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.1672477647562251No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5290.15941273433521275No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.1425372841976477No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4500.1356062957482906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.13168878053778443No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4320.130182043918359No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4090.1232510554690019No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG4080.12294970814511683No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC3990.12023758223015099No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC3950.11903219293461065No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA3900.11752545631518518No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3710.11179985716136848No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3710.11179985716136848No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3680.1108958151897132No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.10878638392251758No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA3460.10426617406424123No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3340.10065000617762014No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA3320.10004731152984997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATG1000.052.251
ATATGAG1200.047.53
GATTGGC1151.8189894E-1245.4347889
GATATGA1201.8189894E-1243.5416682
TGGGATA608.2779996E-539.5833366
ATGTATT500.001614159438.01
GGGGCAG1401.0913936E-1137.3214267
GGTATCA25650.036.6666641
ATACTCA651.3261492E-436.5384646
ATGGGGC1850.035.9459465
CCTTTAG550.002576992634.5454561
GGCATTC550.002576992634.5454567
GACATAT550.002576992634.5454561
CATGGGG6300.033.9285744
GGATGAC1151.0326221E-733.043488
TGGGGCA1753.6379788E-1232.571436
CGAGTAT300.003949759531.66666892-93
ATGAGAG600.00394693831.6666687
ACTCAAT902.4078301E-531.6666688
CATATAG753.0737655E-431.6666643
Produced by FastQC (version 0.11.5)