##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031572_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 331843 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2579502957724 33.0 31.0 34.0 28.0 34.0 2 31.385353917364537 34.0 31.0 34.0 28.0 34.0 3 31.419870239842336 34.0 31.0 34.0 28.0 34.0 4 34.60340884092779 37.0 35.0 37.0 32.0 37.0 5 34.60813396696631 37.0 35.0 37.0 32.0 37.0 6 34.785672742833206 37.0 35.0 37.0 32.0 37.0 7 34.8192036595619 37.0 35.0 37.0 32.0 37.0 8 34.86010854530606 37.0 36.0 37.0 32.0 37.0 9 36.55926447145186 39.0 38.0 39.0 32.0 39.0 10-11 36.57241074845635 39.0 38.0 39.0 32.0 39.0 12-13 36.560305626455886 39.0 38.0 39.0 32.0 39.0 14-15 37.886062083575666 41.0 38.5 41.0 32.0 41.0 16-17 37.8783626594504 41.0 38.5 41.0 32.5 41.0 18-19 37.89382177716571 41.0 38.5 41.0 32.0 41.0 20-21 37.8857306015194 41.0 39.0 41.0 32.5 41.0 22-23 37.785290031731876 41.0 38.5 41.0 32.0 41.0 24-25 37.70010818368928 41.0 38.0 41.0 32.0 41.0 26-27 37.534838764114355 41.0 38.0 41.0 32.0 41.0 28-29 37.38126011396956 40.5 38.0 41.0 31.5 41.0 30-31 37.051281780842146 40.0 37.0 41.0 30.5 41.0 32-33 36.73463957353327 40.0 35.5 41.0 30.0 41.0 34-35 36.52964052277734 40.0 35.0 41.0 29.5 41.0 36-37 36.30158237479772 40.0 35.0 41.0 27.0 41.0 38-39 36.04766259948228 40.0 35.0 41.0 25.0 41.0 40-41 35.82204837829937 40.0 35.0 41.0 24.0 41.0 42-43 35.53319491446256 40.0 35.0 41.0 21.5 41.0 44-45 35.3717767136869 40.0 35.0 41.0 17.0 41.0 46-47 34.92393390850492 40.0 35.0 41.0 14.0 41.0 48-49 34.52206766452811 40.0 35.0 41.0 11.0 41.0 50-51 33.46638169254738 38.5 34.0 40.0 10.0 40.5 52-53 33.47239809186874 38.5 34.5 39.5 10.0 40.5 54-55 34.08026536645342 40.0 35.0 41.0 8.5 41.0 56-57 34.051449028606896 40.0 35.0 41.0 8.0 41.0 58-59 33.84037632253807 40.0 35.0 41.0 8.0 41.0 60-61 33.67216725981865 40.0 34.0 41.0 8.0 41.0 62-63 33.408994614923316 39.0 34.0 41.0 8.0 41.0 64-65 33.161247939537674 39.0 33.0 41.0 8.0 41.0 66-67 32.85238350665827 39.0 33.0 41.0 7.0 41.0 68-69 32.48704055833632 37.5 32.5 41.0 7.0 41.0 70-71 32.10487489565849 37.0 32.0 40.0 7.0 41.0 72-73 31.701972016887503 37.0 31.0 39.5 7.0 41.0 74-75 31.25254261804528 36.0 30.5 39.0 7.0 41.0 76-77 30.866480534469616 36.0 30.0 39.0 7.0 41.0 78-79 30.403947047248245 35.0 29.0 37.0 7.0 40.0 80-81 29.992806839378865 35.0 28.5 37.0 7.0 39.0 82-83 29.616080495897158 35.0 27.5 36.5 7.0 39.0 84-85 29.19973300627104 35.0 26.0 36.0 7.0 38.0 86-87 28.883491590902928 35.0 26.0 36.0 7.0 37.0 88-89 28.570034624807512 35.0 25.0 35.5 7.0 37.0 90-91 28.2677470972719 35.0 24.0 35.0 7.0 36.5 92-93 28.03236319584864 35.0 23.0 35.0 7.0 36.0 94-95 27.820400008437723 35.0 20.5 35.0 7.0 36.0 96-97 27.592296658359526 35.0 20.0 35.0 7.0 36.0 98-99 27.3954113842992 35.0 19.5 35.0 7.0 35.5 100-101 26.095389988639205 33.0 14.5 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.9475744746382375 1101 2 -0.9580955954598629 1101 3 -0.771433964128768 1101 4 -1.1763232995747686 1101 5 -1.4335096053033638 1101 6 -0.9837526318186676 1101 7 -0.9237424035317332 1101 8 -1.0346878394091306 1101 9 -0.7501732518390298 1101 10-11 -0.6263361154199174 1101 12-13 -0.698242586529787 1101 14-15 -0.7545217133155404 1101 16-17 -0.806510507639409 1101 18-19 -0.728477521426413 1101 20-21 -0.6445033230637875 1101 22-23 -0.6870828734791701 1101 24-25 -0.7207697517248519 1101 26-27 -0.6886817981023583 1101 28-29 -0.9429245010968046 1101 30-31 -0.5615540833644417 1101 32-33 -0.5390135202503004 1101 34-35 -0.5705171966094156 1101 36-37 -0.5491152350360551 1101 38-39 -0.4220923098151914 1101 40-41 -0.5283748878477539 1101 42-43 -0.3905073808318704 1101 44-45 -0.21477040442926665 1101 46-47 -0.44544574946851867 1101 48-49 -0.4118679074999463 1101 50-51 -0.016600824074124887 1101 52-53 0.35381454302795134 1101 54-55 0.32124036506915843 1101 56-57 0.12166432834874996 1101 58-59 0.21082737610188218 1101 60-61 0.2995585206486453 1101 62-63 0.4644197240108312 1101 64-65 0.3435844736646132 1101 66-67 0.41707032710943537 1101 68-69 0.664332310455535 1101 70-71 0.2497341526067416 1101 72-73 0.22331389838208437 1101 74-75 0.4073630803310593 1101 76-77 0.6242204918198766 1101 78-79 0.5522640569212278 1101 80-81 0.5506245326699855 1101 82-83 0.5704673427179863 1101 84-85 0.5856814581748004 1101 86-87 0.5049471099831813 1101 88-89 0.47033880020572383 1101 90-91 0.3139214236978489 1101 92-93 0.28842280735965886 1101 94-95 -0.03409777767082289 1101 96-97 0.35680888869632454 1101 98-99 0.7686673518293716 1101 100-101 0.38665221371578085 1102 1 -0.54876132795534 1102 2 -0.30821732400520574 1102 3 -0.3063548405742935 1102 4 -0.672291041510249 1102 5 -0.7903264038426201 1102 6 -0.44510381867198134 1102 7 -0.24495360255789933 1102 8 -0.17087164951259837 1102 9 -0.07731871745071572 1102 10-11 -0.20914956642418048 1102 12-13 -0.07183510022424855 1102 14-15 -0.0344593274360534 1102 16-17 -0.1511544516442811 1102 18-19 -0.08947873871186829 1102 20-21 -0.1670231039524026 1102 22-23 -0.3278315040330355 1102 24-25 -0.43706616073276194 1102 26-27 -0.19937108599036435 1102 28-29 -0.15579729476691284 1102 30-31 -0.11865237916479998 1102 32-33 -0.0754940436830438 1102 34-35 -0.3728224309368642 1102 36-37 -0.5040756732588179 1102 38-39 -0.5091282197360698 1102 40-41 -0.5100395257053307 1102 42-43 -0.5712742706675371 1102 44-45 -0.16912219992531163 1102 46-47 0.06539569910116683 1102 48-49 -0.13630491297772807 1102 50-51 -0.2880174400205675 1102 52-53 -0.21016902361843393 1102 54-55 -0.25263364588641224 1102 56-57 -0.1552681123085904 1102 58-59 -0.19042034148790066 1102 60-61 -0.4838407489800858 1102 62-63 -0.2565936661291559 1102 64-65 -0.0074882591412261945 1102 66-67 -0.6860565748990766 1102 68-69 -0.6118773669638173 1102 70-71 -0.5669883063098702 1102 72-73 -0.4107777023482875 1102 74-75 -0.24247715095317446 1102 76-77 -0.5889946633840211 1102 78-79 -0.5690840867184548 1102 80-81 -0.45036451176093095 1102 82-83 -0.7335877394487831 1102 84-85 -0.6557595034807093 1102 86-87 -0.5809475948250942 1102 88-89 -0.17786570375045585 1102 90-91 -0.2869991484263146 1102 92-93 -0.1867369613561074 1102 94-95 -0.24263399191306334 1102 96-97 -0.37942056961164994 1102 98-99 -0.29310988493264745 1102 100-101 -0.39670901574252326 1103 1 0.044374738016042414 1103 2 -0.02856658425259795 1103 3 -0.11350376126311446 1103 4 -0.221436081529653 1103 5 -0.1843962558921035 1103 6 -0.08947684801954381 1103 7 -0.13611295254577271 1103 8 -0.28341106256134907 1103 9 -0.22460224885988112 1103 10-11 -0.06451883637324585 1103 12-13 -0.08255241771151844 1103 14-15 0.09458808949337083 1103 16-17 -0.2170651962477237 1103 18-19 -0.10551681778050437 1103 20-21 -0.170358054231329 1103 22-23 -0.031475695156004235 1103 24-25 -0.06367300041261359 1103 26-27 0.05106852109184956 1103 28-29 0.11369700549987982 1103 30-31 -0.21847350455892922 1103 32-33 -0.13796372595323447 1103 34-35 -0.109831283713973 1103 36-37 0.09563934008098585 1103 38-39 -0.035353056020333895 1103 40-41 0.038620430637131165 1103 42-43 -0.1921813771433989 1103 44-45 -0.3144653966267441 1103 46-47 -0.20659981387481707 1103 48-49 -0.5170955993711246 1103 50-51 -0.6198601516383206 1103 52-53 -0.518560971229391 1103 54-55 -0.8365834397258496 1103 56-57 -0.5325419420442188 1103 58-59 -0.5749401086477306 1103 60-61 -0.8026013601855198 1103 62-63 -0.31647118250073447 1103 64-65 -0.36261498725183117 1103 66-67 -0.3765883478799239 1103 68-69 -0.30371280232641595 1103 70-71 -0.6189528952014527 1103 72-73 -0.6296262349701642 1103 74-75 -0.6113984461265964 1103 76-77 -0.3760277703449688 1103 78-79 -0.55823033944705 1103 80-81 -0.42632255202287794 1103 82-83 -0.5784367571543392 1103 84-85 -0.2867472551178665 1103 86-87 -0.49487555989906795 1103 88-89 -0.17865396472547346 1103 90-91 -0.1918772711524852 1103 92-93 0.005492604494005349 1103 94-95 -0.30881759608478276 1103 96-97 -0.15306839886219592 1103 98-99 -0.13163765597315447 1103 100-101 -0.5115070996620013 1104 1 1.0267697499360864 1104 2 1.1145656365432934 1104 3 1.0663972106789394 1104 4 0.961307362446135 1104 5 0.8776796656189916 1104 6 1.000748715890225 1104 7 0.8522420813805027 1104 8 0.9713111087205135 1104 9 1.263386014481398 1104 10-11 1.214272654549184 1104 12-13 1.360185524705507 1104 14-15 1.3756718965720367 1104 16-17 1.2960468521659934 1104 18-19 1.407641076956338 1104 20-21 1.4624587438614611 1104 22-23 1.4069526928872804 1104 24-25 1.470711741931055 1104 26-27 1.5300250587775324 1104 28-29 1.4115147205191434 1104 30-31 1.3995349311645242 1104 32-33 1.4468519718278046 1104 34-35 1.3992004419416304 1104 36-37 1.352378749580339 1104 38-39 1.430078816343503 1104 40-41 1.4239129301786662 1104 42-43 1.0889482762583995 1104 44-45 0.9397066187776204 1104 46-47 1.0061271254246478 1104 48-49 1.0670782498861584 1104 50-51 0.9924633400176504 1104 52-53 1.0140357645123999 1104 54-55 1.0543940038831678 1104 56-57 1.1054096442385557 1104 58-59 1.219760056399032 1104 60-61 1.1367898006433848 1104 62-63 1.3841981738168911 1104 64-65 1.0668275214589755 1104 66-67 0.968496926281091 1104 68-69 0.91607447073428 1104 70-71 0.8893452892841509 1104 72-73 0.8730328517710824 1104 74-75 1.0622674258704699 1104 76-77 0.791632233322737 1104 78-79 0.6280891177324825 1104 80-81 0.8874038371207149 1104 82-83 1.0756527348684024 1104 84-85 1.0588423285975175 1104 86-87 1.1322562941262397 1104 88-89 1.0751971264170166 1104 90-91 0.8381062241711916 1104 92-93 0.5991107963150206 1104 94-95 0.4575036949476754 1104 96-97 0.5722204329734915 1104 98-99 0.5218558338489601 1104 100-101 0.5690448900684224 1105 1 0.4542494247328328 1105 2 0.1466213856722156 1105 3 0.0779999981353825 1105 4 0.23701003375856544 1105 5 0.33397467142619064 1105 6 0.21027252541404096 1105 7 0.21569155873242352 1105 8 0.3582681354336401 1105 9 0.3483802072804707 1105 10-11 0.2903934443285081 1105 12-13 0.26200898579725873 1105 14-15 0.2886858338542666 1105 16-17 0.3742756558826059 1105 18-19 0.31740298171824577 1105 20-21 0.3214715197035076 1105 22-23 0.3994265604830929 1105 24-25 0.5052994306650831 1105 26-27 0.5165697742246849 1105 28-29 0.4783962536201827 1105 30-31 0.7562132122330496 1105 32-33 0.5318984294352376 1105 34-35 0.4169356335792642 1105 36-37 0.5972684127626877 1105 38-39 0.8805491996187698 1105 40-41 1.0690464958000376 1105 42-43 0.7892096003002038 1105 44-45 1.0230283377090998 1105 46-47 1.1059064517893376 1105 48-49 1.4342327214308739 1105 50-51 1.3214180438504002 1105 52-53 1.2552610839084508 1105 54-55 1.3934416229307942 1105 56-57 1.4844630704871093 1105 58-59 1.2700097660389886 1105 60-61 1.3883635520951856 1105 62-63 0.9924385919353611 1105 64-65 1.1911945365576955 1105 66-67 1.2971961132729604 1105 68-69 1.4476656437888664 1105 70-71 1.5189794356256172 1105 72-73 1.3079050933506373 1105 74-75 1.3767970425952036 1105 76-77 1.164849422637488 1105 78-79 1.2807546229589661 1105 80-81 1.3277000043681042 1105 82-83 1.1986434433469135 1105 84-85 0.984643722325746 1105 86-87 0.9871169213039366 1105 88-89 1.2482633285076048 1105 90-91 1.334801926842502 1105 92-93 1.343988845095506 1105 94-95 1.4404305242159658 1105 96-97 1.3011170647969514 1105 98-99 1.5225352763576723 1105 100-101 1.4412748552252168 1106 1 0.43655711704052536 1106 2 0.6555957446465719 1106 3 0.5072307673661491 1106 4 0.20508695683548694 1106 5 0.30820544065696254 1106 6 0.3871956023371155 1106 7 0.6433838664247276 1106 8 0.47801172517722534 1106 9 0.7472263611266214 1106 10-11 0.504560110995179 1106 12-13 0.5352782165664891 1106 14-15 0.6054807056491427 1106 16-17 0.4907500148569639 1106 18-19 0.4149670842823383 1106 20-21 0.4348689556009404 1106 22-23 0.5057086117651437 1106 24-25 0.2528635332291813 1106 26-27 0.5079800306349398 1106 28-29 0.48538343310736565 1106 30-31 0.5119824430022746 1106 32-33 0.48157791661472515 1106 34-35 0.47514076178438813 1106 36-37 0.8017555922498687 1106 38-39 0.524267148336719 1106 40-41 0.343661880415425 1106 42-43 0.27927370286430886 1106 44-45 0.5436052607860233 1106 46-47 0.6196244005072913 1106 48-49 0.6632711829693392 1106 50-51 0.43789240282476527 1106 52-53 0.34519698134434407 1106 54-55 0.30164675113592665 1106 56-57 0.33837332689736144 1106 58-59 0.27520207373129324 1106 60-61 0.16669688542851446 1106 62-63 0.35641295090971425 1106 64-65 0.24613043399359924 1106 66-67 0.12437560045244567 1106 68-69 0.16914000276322838 1106 70-71 0.143723025369205 1106 72-73 0.0655974010429432 1106 74-75 -0.2130106497124835 1106 76-77 -0.17739416710610456 1106 78-79 0.13190846911281184 1106 80-81 0.20673846590656098 1106 82-83 -0.1149463002428277 1106 84-85 0.06772064540266598 1106 86-87 0.13865538284239776 1106 88-89 -0.1905828253385451 1106 90-91 0.018263465304038107 1106 92-93 0.1395657681724316 1106 94-95 -0.10796691168146921 1106 96-97 0.0611811673610525 1106 98-99 0.2106121994345962 1106 100-101 0.20864665009701255 1107 1 0.09880429015726477 1107 2 -0.024807185756358763 1107 3 -0.058583852796292746 1107 4 0.13113011657429752 1107 5 -0.023395928987888226 1107 6 0.01317107613867563 1107 7 0.1446977699125469 1107 8 0.09220188284565012 1107 9 0.11848372694920783 1107 10-11 0.07637688118150976 1107 12-13 0.23062182223618066 1107 14-15 0.45573552329526024 1107 16-17 0.08687399956790642 1107 18-19 0.2554671639128543 1107 20-21 0.17068477022110073 1107 22-23 0.2269420884334039 1107 24-25 0.3317176501267838 1107 26-27 0.09887826283752332 1107 28-29 -0.045423622156214094 1107 30-31 0.1179109348003351 1107 32-33 0.30327524102943926 1107 34-35 0.19942010562895263 1107 36-37 0.11010485168608852 1107 38-39 -0.1107862041079386 1107 40-41 -0.11911085409644073 1107 42-43 -0.15908232516563459 1107 44-45 0.12816291327845875 1107 46-47 0.11008864640631799 1107 48-49 0.3770691603542744 1107 50-51 0.28582798173859914 1107 52-53 0.49525073194158153 1107 54-55 0.6739592212744796 1107 56-57 0.9366473354974616 1107 58-59 0.8480739482335977 1107 60-61 0.7935084796314129 1107 62-63 0.8266311385192111 1107 64-65 0.8904906028102886 1107 66-67 1.0259124693806214 1107 68-69 1.0758229936853496 1107 70-71 1.1875198082964218 1107 72-73 1.1976048863113 1107 74-75 1.2785982848312365 1107 76-77 1.1994871037969048 1107 78-79 0.9938291571204552 1107 80-81 0.9528863397718297 1107 82-83 0.87424385742559 1107 84-85 0.7975940328847493 1107 86-87 1.0800672318629445 1107 88-89 1.1845366204330716 1107 90-91 1.2380835003414674 1107 92-93 1.264237292300475 1107 94-95 0.99602058632777 1107 96-97 1.0733944975091667 1107 98-99 1.3170383819477358 1107 100-101 1.410281066405343 1108 1 -0.1909357604523514 1108 2 -0.2681934291425989 1108 3 -0.09533333519794951 1108 4 0.07984953993140209 1108 5 -0.20330927919108888 1108 6 -0.1804682153267052 1108 7 -0.2970244906502941 1108 8 0.002465666297837288 1108 9 0.014676503576765754 1108 10-11 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66-67 -0.3147812878569809 2316 68-69 -0.11850667259531633 2316 70-71 -0.4100036152218465 2316 72-73 0.09229774871786489 2316 74-75 0.26213602564605765 2316 76-77 0.42153021359793996 2316 78-79 0.5749495382132004 2316 80-81 0.4390559365714921 2316 82-83 0.368756437697197 2316 84-85 0.471974230800317 2316 86-87 0.35084573486325965 2316 88-89 0.21538197257540403 2316 90-91 0.45684994944137003 2316 92-93 0.6889464722141518 2316 94-95 0.6054728970973251 2316 96-97 0.46461988965571166 2316 98-99 0.44749290347631643 2316 100-101 0.6913172281065734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 47.0 7 1062.0 8 2707.0 9 1973.0 10 1736.0 11 2016.0 12 2222.0 13 2101.0 14 1920.0 15 2152.0 16 2702.0 17 3328.0 18 3790.0 19 4855.0 20 6487.0 21 7348.0 22 6222.0 23 4777.0 24 3951.0 25 3290.0 26 3232.0 27 3288.0 28 3292.0 29 3369.0 30 3686.0 31 4341.0 32 5102.0 33 6440.0 34 8948.0 35 15400.0 36 21197.0 37 45507.0 38 101547.0 39 41407.0 40 401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.862224003519735 24.385628143429273 17.22441033862399 18.527737514427002 2 14.222086950756834 26.437803419086737 41.14988111848073 18.190228511675702 3 14.631316616592787 34.52566424483867 33.5185614884147 17.32445765015384 4 11.691070777445962 22.447060808876486 42.27450933121988 23.587359082457667 5 9.028365823597303 42.11841141744741 36.65920329794511 12.194019461010177 6 20.395186880542905 44.29082427533502 20.762830615682716 14.551158228439354 7 20.888793797066686 37.76273719801232 27.528078036902993 13.820390968018009 8 21.94411212531227 36.55825194444361 24.27955388542172 17.2180820448224 9 25.49910650518468 18.911654005056608 24.20903861163261 31.380200878126104 10-11 20.258224521837136 30.687554054176218 31.43353935445376 17.62068206953288 12-13 22.671865912494766 31.032144719038822 30.02790476219176 16.268084606274655 14-15 20.566502834171583 32.13085706192386 29.420840578225242 17.881799525679313 16-17 16.8816277577047 33.70931434443396 31.34268313630241 18.06637476155893 18-19 16.531462167350224 31.734434657353027 35.101689654445025 16.632413520851728 20-21 17.269160416220924 31.74874865523757 34.97241165249832 16.00967927604319 22-23 18.28771437095253 30.926823829340982 34.73600467691046 16.04945712279602 24-25 17.479199500968832 32.14215758656955 33.92733913326483 16.451303779196788 26-27 16.03634851420702 32.572180217753576 34.795671447039716 16.59579982099969 28-29 16.803729474480402 31.11124839155866 35.981925187513376 16.103096946447568 30-31 17.495170909134742 31.893998065350182 34.48136618822756 16.129464837287514 32-33 15.899386155501247 33.39169426505908 34.64786058467408 16.0610589947656 34-35 16.642960677187705 32.35279936596523 35.00977872066007 15.994461236186991 36-37 17.17483870384489 31.826194917476037 33.78359645977164 17.215369918907435 38-39 15.622297291188906 33.48270115687237 34.40452262063687 16.49047893130185 40-41 15.924548657045651 32.077669259258144 35.77987180684842 16.217910276847785 42-43 16.379884463436024 32.125432810093926 33.83919504102843 17.65548768544161 44-45 15.995515951820591 32.6940752102651 33.97766413635364 17.332744701560678 46-47 16.902295516698434 31.722880583409296 34.25766741752488 17.11715648236739 48-49 16.538342157840653 31.925379925928382 33.44282352090308 18.093454395327882 50-51 15.808228590026005 32.15903303670712 32.802258899539844 19.230479473727033 52-53 16.421621067794106 31.890984592111334 33.59389831938597 18.09349602070859 54-55 16.922912341076955 31.58677446864933 32.54385356930838 18.946459620965335 56-57 16.283603993454737 32.48011861030668 31.894751433659895 19.34152596257869 58-59 16.31750556739181 30.951383636237615 32.977492368379025 19.75361842799155 60-61 17.411848374080513 31.305617415464543 31.94432306843899 19.338211142015954 62-63 16.102343578137855 31.66648083581694 32.95187784584879 19.27929774019642 64-65 17.15464843314459 30.348387641143553 33.006572385133936 19.490391540577924 66-67 17.130841994557667 30.774492757117073 31.903490506052563 20.191174742272704 68-69 16.535078335236843 31.498178355427115 31.87983474112758 20.08690856820846 70-71 17.578192096865084 30.273954852143937 33.103756897086875 19.044096153904107 72-73 17.765479458659666 30.094050499784537 31.888875160844133 20.25159488071166 74-75 16.908146322206584 31.204967409286922 31.39421352868676 20.492672739819735 76-77 17.51445713786339 30.52708660420741 32.542045485365065 19.416410772564134 78-79 17.90364720666098 30.28450200847991 31.66105658398701 20.1507942008721 80-81 17.573521213344865 31.07448401804468 31.094372941421096 20.257621827189364 82-83 18.595691335963092 30.42703929267756 31.211747724074336 19.765521647285013 84-85 18.16039512661108 30.125390621468583 30.64822822840922 21.06598602351112 86-87 17.802243832173648 31.155998469155595 30.768465810639366 20.273291888031387 88-89 19.628257941255352 29.581760049179884 30.65018698601447 20.139795023550295 90-91 19.69093818462345 29.460919772301963 30.471036001964784 20.3771060411098 92-93 19.90278535331467 30.490020883369546 29.641577492971074 19.96561627034471 94-95 20.569064286424606 28.642008419644228 30.2979119643928 20.491015329538367 96-97 21.05001461534521 28.638542925419554 29.37307702738946 20.93836543184578 98-99 20.406939426174425 29.515463637925166 29.336764674861303 20.740832261039106 100-101 21.95782342854904 28.339154359139712 29.719626449857312 19.98339576245393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2678.0 1 1603.5 2 523.0 3 462.5 4 334.0 5 318.0 6 502.5 7 860.5 8 1330.0 9 1524.5 10 1151.0 11 800.5 12 785.0 13 773.5 14 749.0 15 708.0 16 687.5 17 709.0 18 737.5 19 809.5 20 929.0 21 1069.0 22 1239.0 23 1477.5 24 1720.0 25 2095.5 26 2902.0 27 4031.5 28 4806.0 29 5621.5 30 7283.0 31 8937.5 32 11725.0 33 17838.0 34 23298.5 35 22593.5 36 21707.5 37 22333.0 38 20058.5 39 17690.5 40 15472.5 41 13092.5 42 11124.0 43 9950.0 44 9595.5 45 9276.0 46 7962.5 47 6242.5 48 5444.5 49 4662.0 50 3979.0 51 3401.0 52 2606.5 53 1929.0 54 1471.0 55 1152.5 56 954.0 57 837.0 58 755.0 59 726.0 60 652.0 61 585.5 62 536.0 63 456.0 64 410.0 65 359.0 66 291.0 67 260.5 68 233.0 69 189.5 70 168.0 71 163.0 72 144.0 73 120.0 74 93.0 75 80.5 76 65.0 77 45.5 78 49.5 79 46.0 80 37.0 81 30.5 82 28.5 83 24.0 84 14.0 85 14.5 86 13.0 87 8.5 88 6.5 89 6.0 90 6.0 91 4.0 92 2.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.5067366194254511E-4 48-49 6.026946477701805E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 331843.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.41451228442366 #Duplication Level Percentage of deduplicated Percentage of total 1 79.31769285933818 23.330912509831457 2 6.854830447700031 4.032629888230278 3 3.1195574223952462 2.752807803690299 4 1.8235836492162687 2.1455929460618424 5 1.2672881876856879 1.863833198229283 6 0.881057268722467 1.5549521912470656 7 0.6956254482122733 1.432303830425834 8 0.56654031349247 1.3331605608676391 9 0.5429771539801249 1.4374267349318803 >10 4.054912406515726 24.662264986755787 >50 0.567564798688659 11.557875260288752 >100 0.29402725130621865 14.763005397130572 >500 0.0051224259809445755 0.9579831426307018 >1k 0.00819588156951132 6.660981247156035 >5k 0.001024485196188915 1.5142703025225783 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 5025 1.5142703025225783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4292 1.2933827141148073 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 3985 1.2008690856820845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3712 1.118601266261455 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2915 0.878427449125038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2237 0.6741139635309469 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.6192687505838604 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 1476 0.44478865005439316 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 1432 0.4315293678034492 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 817 0.2462007636141187 No Hit AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG 690 0.20792965348071227 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 588 0.17719222644443305 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 555 0.1672477647562251 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 529 0.15941273433521275 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.1425372841976477 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 450 0.1356062957482906 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.13168878053778443 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 432 0.130182043918359 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 409 0.1232510554690019 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 408 0.12294970814511683 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 399 0.12023758223015099 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 395 0.11903219293461065 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 390 0.11752545631518518 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 371 0.11179985716136848 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 371 0.11179985716136848 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 368 0.1108958151897132 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.10878638392251758 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 346 0.10426617406424123 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 334 0.10065000617762014 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 332 0.10004731152984997 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0134732388509023E-4 0.0 10-11 0.0 0.0 0.0 4.5202098582763537E-4 0.0 12-13 0.0 0.0 0.0 6.026946477701805E-4 0.0 14-15 0.0 0.0 0.0 6.026946477701805E-4 0.0 16-17 0.0 0.0 0.0 6.026946477701805E-4 0.0 18-19 0.0 0.0 0.0 6.026946477701805E-4 0.0 20-21 0.0 0.0 0.0 0.0018080839433105413 0.0 22-23 0.0 0.0 0.0 0.010245809012093068 0.0 24-25 0.0 0.0 0.0 0.029984058726566478 0.0 26-27 0.0 0.0 0.0 0.0681044951980304 0.0 28-29 0.0 0.0 0.0 0.12566183406008263 0.0 30-31 0.0 0.0 0.0 0.2275172295332431 0.0 32-33 0.0 0.0 0.0 0.3727666396458566 0.0 34-35 0.0 0.0 0.0 0.6281584966384706 0.0 36-37 0.0 0.0 0.0 1.0248822485331919 0.0 38-39 0.0 0.0 0.0 1.4383307769035358 0.0 40-41 0.0 0.0 0.0 1.7969340923267931 0.0 42-43 0.0 0.0 0.0 2.129320190572048 0.0 44-45 0.0 0.0 0.0 2.496662578387973 0.0 46-47 0.0 0.0 0.0 2.9736953920980707 0.0 48-49 0.0 0.0 0.0 3.403718023282094 0.0 50-51 0.0 0.0 0.0 3.777690052223491 0.0 52-53 0.0 0.0 0.0 4.161305195529211 0.0 54-55 0.0 0.0 0.0 4.565411956859117 0.0 56-57 0.0 0.0 0.0 5.071072766338299 0.0 58-59 0.0 0.0 0.0 5.565433051171789 0.0 60-61 0.0 0.0 0.0 6.018508752633022 0.0 62-63 0.0 0.0 0.0 6.4479286891692755 0.0 64-65 0.0 0.0 0.0 6.950425351747664 0.0 66-67 0.0 0.0 0.0 7.495713334317735 0.0 68-69 0.0 0.0 0.0 8.089668909695247 0.0 70-71 0.0 0.0 0.0 8.715115280418752 0.0 72-73 0.0 0.0 0.0 9.305304014247701 0.0 74-75 0.0 0.0 0.0 9.895191400752765 0.0 76-77 0.0 0.0 0.0 10.571264121888966 0.0 78-79 0.0 0.0 0.0 11.30323677160585 0.0 80-81 0.0 0.0 0.0 11.968762336406073 0.0 82-83 0.0 0.0 0.0 12.623590071208373 0.0 84-85 0.0 0.0 0.0 13.317592958115736 0.0 86-87 0.0 0.0 0.0 14.04730550290348 0.0 88-89 0.0 0.0 0.0 14.779127478958422 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATATG 100 0.0 52.25 1 ATATGAG 120 0.0 47.5 3 GATTGGC 115 1.8189894E-12 45.434788 9 GATATGA 120 1.8189894E-12 43.541668 2 TGGGATA 60 8.2779996E-5 39.583336 6 ATGTATT 50 0.0016141594 38.0 1 GGGGCAG 140 1.0913936E-11 37.321426 7 GGTATCA 2565 0.0 36.666664 1 ATACTCA 65 1.3261492E-4 36.538464 6 ATGGGGC 185 0.0 35.945946 5 CCTTTAG 55 0.0025769926 34.545456 1 GGCATTC 55 0.0025769926 34.545456 7 GACATAT 55 0.0025769926 34.545456 1 CATGGGG 630 0.0 33.928574 4 GGATGAC 115 1.0326221E-7 33.04348 8 TGGGGCA 175 3.6379788E-12 32.57143 6 CGAGTAT 30 0.0039497595 31.666668 92-93 ATGAGAG 60 0.003946938 31.666668 7 ACTCAAT 90 2.4078301E-5 31.666668 8 CATATAG 75 3.0737655E-4 31.666664 3 >>END_MODULE