FastQCFastQC Report
Fri 10 Feb 2017
SRR2031572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331843
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT110363.325669066395856No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA54991.6571089340441114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47451.429893051834753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41791.259330466515792No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT38101.148133304002194No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT32280.9727491615010712TruSeq Adapter, Index 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26930.8115283432225481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26200.7895299885789363No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.7286578291541482No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT21340.6430751891707825RNA PCR Primer, Index 1 (95% over 23bp)
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT14050.42339299005855174No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC13080.3941622996416981No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA8120.24469402699469328No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT8090.243789985023038TruSeq Adapter, Index 1 (96% over 26bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG6950.20943639010013773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.19376632925811302No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC5990.18050704700716905No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.17146662729061635No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.16543968081291455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5190.15639926109636185No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG5000.1506736619425451TruSeq Adapter, Index 1 (96% over 28bp)
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4680.14103054757822223No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG4620.1392224636349117TruSeq Adapter, Index 1 (95% over 24bp)
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4470.13470225377663533No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.1304833912422441No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC4260.12837395997504844No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC4240.12777126532727826No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4240.12777126532727826No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG4130.12445644476454228No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC4110.12385375011677209No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA4100.123552402792887No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3990.12023758223015099No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3830.11541602504798958No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA3810.11481333040021938No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3650.10999177321805793No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3580.1078823419508623No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT3520.10607425800755177No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3430.10336213209258595No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA3410.10275943744481578No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3350.10095135350150523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGAG250.005018610556.998645
CCTTTAG707.7601726E-847.606481
CTTTAGG751.3591307E-744.3322752
GGTATCA24900.041.4883771
TGGGAAC704.3016153E-640.7133186
TTAGGCA951.9215804E-839.9990464
CTATACA853.5670564E-739.2053341
GATCCTC500.001596495738.0851821
GTACCCA1054.5498382E-836.2716061
CATTCCG958.6767614E-734.9991659
GGGGCAG1106.988557E-834.5446367
ACCCATG1001.2957444E-633.2492073
GTATCAA38000.032.8234181
CATATAG753.074194E-431.6659133
GGCATTC1201.500648E-731.6659137
GGATATG804.4283556E-429.754051
CTTTACA1951.6370905E-1129.2962931
CAGTACA650.005838840729.2300729
AGGCATT1153.8543967E-628.9123556
TGGGGCA1503.322384E-828.4993216
Produced by FastQC (version 0.11.5)