##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031572_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 331843 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.635128660239936 34.0 31.0 34.0 31.0 34.0 2 32.78821912772003 34.0 31.0 34.0 31.0 34.0 3 32.84834093230835 34.0 31.0 34.0 31.0 34.0 4 36.294678507607514 37.0 37.0 37.0 35.0 37.0 5 36.23311324933779 37.0 37.0 37.0 35.0 37.0 6 36.27942430607245 37.0 37.0 37.0 35.0 37.0 7 36.29537160645245 37.0 37.0 37.0 35.0 37.0 8 36.326476677223866 37.0 37.0 37.0 35.0 37.0 9 38.13629939459322 39.0 39.0 39.0 37.0 39.0 10-11 38.110969645284065 39.0 39.0 39.0 37.0 39.0 12-13 38.118370735558685 39.0 39.0 39.0 37.0 39.0 14-15 39.553746801951526 41.0 39.5 41.0 37.0 41.0 16-17 39.50173425384896 41.0 39.0 41.0 36.5 41.0 18-19 39.5414352570342 41.0 39.5 41.0 36.5 41.0 20-21 39.5609038008938 41.0 40.0 41.0 36.0 41.0 22-23 39.49928731357901 41.0 39.5 41.0 36.0 41.0 24-25 39.389304580780674 41.0 39.0 41.0 35.0 41.0 26-27 39.21364018526833 41.0 39.0 41.0 35.0 41.0 28-29 39.06750933423336 41.0 39.0 41.0 35.0 41.0 30-31 38.78892729393117 40.5 38.0 41.0 35.0 41.0 32-33 37.74560560264945 39.5 36.5 41.0 33.5 41.0 34-35 37.829315369014864 40.0 36.5 41.0 34.0 41.0 36-37 37.87828280240957 40.0 37.0 41.0 34.0 41.0 38-39 37.598777132559675 40.0 36.0 41.0 33.0 41.0 40-41 37.49518446976431 40.0 36.0 41.0 33.0 41.0 42-43 37.18194899395196 40.0 35.5 41.0 33.0 41.0 44-45 36.86745840653562 40.0 35.0 41.0 31.5 41.0 46-47 36.38411688659999 40.0 35.0 41.0 28.5 41.0 48-49 35.97773193950151 40.0 35.0 41.0 23.5 41.0 50-51 35.72377449577059 40.0 35.0 41.0 20.0 41.0 52-53 35.874900178698965 40.0 36.0 41.0 20.0 41.0 54-55 35.70337478868019 40.0 35.5 41.0 18.0 41.0 56-57 35.44920489508593 40.0 35.0 41.0 15.5 41.0 58-59 35.27124875317544 40.0 35.0 41.0 15.0 41.0 60-61 34.88531775568567 40.0 35.0 41.0 11.0 41.0 62-63 34.47302188082919 39.0 35.0 41.0 10.0 41.0 64-65 34.13042462851409 39.0 35.0 41.0 10.0 41.0 66-67 33.56261696043008 39.0 34.0 41.0 9.0 41.0 68-69 32.82820339738973 37.5 33.0 40.5 8.0 41.0 70-71 32.210091820529584 37.0 32.0 40.0 7.0 41.0 72-73 31.70014434536814 37.0 31.5 39.0 7.0 41.0 74-75 31.25975837971571 36.0 31.0 39.0 7.0 41.0 76-77 29.93314910966933 34.5 28.5 37.0 7.0 39.0 78-79 30.544860069370152 35.0 30.0 37.0 7.0 39.5 80-81 30.376223093450818 35.0 30.0 37.0 7.0 39.0 82-83 30.09498919669844 35.0 30.0 36.5 7.0 39.0 84-85 29.757095976109184 35.0 29.0 36.0 7.0 38.0 86-87 29.49108463942286 35.0 29.0 36.0 7.0 37.0 88-89 29.226448953270072 35.0 29.0 35.5 7.0 37.0 90-91 29.013388861600212 35.0 28.5 35.0 7.0 36.5 92-93 28.874428570137084 35.0 27.5 35.0 7.0 36.0 94-95 28.73895938742116 35.0 27.0 35.0 7.0 36.0 96-97 28.6016956813915 35.0 26.5 35.0 7.0 36.0 98-99 28.518743803545654 35.0 26.0 35.0 7.0 35.5 100-101 27.58531444086511 33.5 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5325234521860267 1101 2 0.4520949065972104 1101 3 0.5055129976314774 1101 4 0.31360079943404884 1101 5 0.31569654013453885 1101 6 0.35170715799606 1101 7 0.23776255014741565 1101 8 0.15976541901556374 1101 9 0.46123750179095424 1101 10-11 0.5092503142729683 1101 12-13 0.37440748499064824 1101 14-15 0.5894449797241066 1101 16-17 0.572260039491276 1101 18-19 0.5169134534542863 1101 20-21 0.562481576356781 1101 22-23 0.47162847811144104 1101 24-25 0.6064454788676983 1101 26-27 0.7280034167536442 1101 28-29 0.830732172395912 1101 30-31 0.758124997069622 1101 32-33 0.7491710204186361 1101 34-35 0.4471726258030557 1101 36-37 0.4615799138428045 1101 38-39 0.9753205920120038 1101 40-41 0.7972898034478888 1101 42-43 0.7211951796812244 1101 44-45 0.943898792273508 1101 46-47 1.3284779342312376 1101 48-49 1.629714687118053 1101 50-51 1.6136549620415934 1101 52-53 1.7862563055828602 1101 54-55 1.7660629248534292 1101 56-57 1.7370773429830493 1101 58-59 1.7880140381193428 1101 60-61 1.885078959217907 1101 62-63 1.8570966685479533 1101 64-65 1.6402499637172454 1101 66-67 1.3027044830039927 1101 68-69 1.2064608838918218 1101 70-71 0.8937689181052164 1101 72-73 0.6094278053691049 1101 74-75 0.6693390728961326 1101 76-77 1.1189686253210631 1101 78-79 1.018994120091378 1101 80-81 0.9647247008919599 1101 82-83 0.7281794620314272 1101 84-85 0.5866514627410169 1101 86-87 0.37110308293481253 1101 88-89 0.3748959873463278 1101 90-91 0.459698714508594 1101 92-93 0.9585496688321413 1101 94-95 0.5631803874833565 1101 96-97 0.6230092029809953 1101 98-99 0.3835961367342904 1101 100-101 0.4688858019347606 1102 1 0.012514322545122525 1102 2 0.12274311110116543 1102 3 0.019968262391067526 1102 4 0.10628187064524752 1102 5 0.06417493331773727 1102 6 0.07918737710743784 1102 7 0.08910156414619763 1102 8 0.09319512078062786 1102 9 0.21762825042151235 1102 10-11 0.2611523227939685 1102 12-13 0.1807449773826093 1102 14-15 0.24715952628527305 1102 16-17 0.2886705690104563 1102 18-19 0.14967364821995943 1102 20-21 0.27002874616810146 1102 22-23 0.04987710866530648 1102 24-25 0.11302642835034504 1102 26-27 0.14435308200014418 1102 28-29 0.22338281147077055 1102 30-31 0.20909121739828862 1102 32-33 0.12763267592685423 1102 34-35 0.011688754835311954 1102 36-37 0.022786548048529198 1102 38-39 0.06908961209721554 1102 40-41 0.10423593856658186 1102 42-43 0.2200159343982051 1102 44-45 0.05786714285171968 1102 46-47 0.020656875192891277 1102 48-49 0.03160908760496994 1102 50-51 0.09421643495699072 1102 52-53 0.1574522885408598 1102 54-55 0.19119986946694212 1102 56-57 -0.11688644277471383 1102 58-59 -0.08466551148504209 1102 60-61 0.13743744978394545 1102 62-63 -0.02551136553603328 1102 64-65 0.22085708483714228 1102 66-67 -0.004127531603430157 1102 68-69 -0.15727922566386354 1102 70-71 -0.11068178183391808 1102 72-73 0.07762622289801513 1102 74-75 -0.06202124359808536 1102 76-77 -0.3355353308566613 1102 78-79 -0.1534039322518943 1102 80-81 0.021176313795184143 1102 82-83 0.20706716744835063 1102 84-85 0.21838457290535018 1102 86-87 0.2863495832391365 1102 88-89 0.18868935314060664 1102 90-91 -0.026568783969796783 1102 92-93 0.37079099567328555 1102 94-95 0.20697223167081802 1102 96-97 0.2468679978683923 1102 98-99 -0.14615127653411975 1102 100-101 0.01635993461887253 1103 1 0.31657083479348813 1103 2 0.28849013996364903 1103 3 0.19678126263360696 1103 4 0.25110966593993567 1103 5 0.23838060879675993 1103 6 0.33337122382099693 1103 7 0.09940959228104873 1103 8 0.1869678590779884 1103 9 0.19949824799608962 1103 10-11 0.32112079235496793 1103 12-13 0.2567666848771566 1103 14-15 0.32974502228333336 1103 16-17 0.38430784769102644 1103 18-19 0.43754049275064233 1103 20-21 0.3217204754914107 1103 22-23 0.3648934802394095 1103 24-25 0.4549346262644889 1103 26-27 0.3610187380758205 1103 28-29 0.38361201350812024 1103 30-31 0.3305288841458065 1103 32-33 0.8000647642120811 1103 34-35 0.34566886640455863 1103 36-37 0.13928242483727615 1103 38-39 0.16190734190561074 1103 40-41 0.19365808263643203 1103 42-43 0.07198961860872544 1103 44-45 -0.10348381518853955 1103 46-47 -0.034339486679535014 1103 48-49 0.17860460058095384 1103 50-51 -0.11277786530980194 1103 52-53 0.04973339453164982 1103 54-55 0.04952511807233151 1103 56-57 -0.006999831084193886 1103 58-59 0.20103361536433795 1103 60-61 0.03029459264108425 1103 62-63 -0.046400281374019414 1103 64-65 -0.172773160130113 1103 66-67 -0.4779026953191732 1103 68-69 -0.578544081351474 1103 70-71 -0.7111395795540219 1103 72-73 -0.9106104979363288 1103 74-75 -0.3656836253589404 1103 76-77 -0.1143978096342515 1103 78-79 -0.09616405837364894 1103 80-81 -0.34260612617570985 1103 82-83 -0.35679713039302996 1103 84-85 -0.8343834358261546 1103 86-87 -0.5998679767899731 1103 88-89 -0.6016787040992639 1103 90-91 -0.42147237358900824 1103 92-93 -0.5955714831043046 1103 94-95 -0.7065919206454545 1103 96-97 -0.4791506778531698 1103 98-99 -0.48789272693431585 1103 100-101 -0.6226165388227969 1104 1 0.44714116305316054 1104 2 0.4271294210932979 1104 3 0.24436974603723627 1104 4 0.19967009139381275 1104 5 0.19913615620185965 1104 6 0.3515263677784546 1104 7 0.4140987542102579 1104 8 0.3111197021001786 1104 9 0.42047752545803974 1104 10-11 0.43587413913655126 1104 12-13 0.3755086617706098 1104 14-15 0.5062075199910225 1104 16-17 0.4101651739334571 1104 18-19 0.7225212372949272 1104 20-21 0.5779555754363983 1104 22-23 0.6258507514662526 1104 24-25 0.47441931359256273 1104 26-27 0.3727095504726705 1104 28-29 0.5344005702658734 1104 30-31 0.47182725201670195 1104 32-33 1.0377779496146147 1104 34-35 0.9828869115173404 1104 36-37 0.673392089242185 1104 38-39 0.8120998964627333 1104 40-41 0.7042485832782788 1104 42-43 0.08913614345743781 1104 44-45 0.22078722827377106 1104 46-47 0.21190111482422225 1104 48-49 0.014658149086365313 1104 50-51 -0.11529941308167224 1104 52-53 -0.10531887027671161 1104 54-55 -0.16095535139401562 1104 56-57 -0.2405868474054884 1104 58-59 -0.23597353666207255 1104 60-61 -0.09601202756796567 1104 62-63 0.35443412170685207 1104 64-65 0.5450885673593433 1104 66-67 0.6498710072298834 1104 68-69 0.7217951814396528 1104 70-71 0.6858437323843631 1104 72-73 0.887253063406046 1104 74-75 0.7770818248519511 1104 76-77 0.5609185299953126 1104 78-79 0.7109604602399564 1104 80-81 0.5932090214494501 1104 82-83 0.2541968178923142 1104 84-85 0.1989468410856361 1104 86-87 0.3075224504977605 1104 88-89 0.30594095480408967 1104 90-91 0.5059070505811754 1104 92-93 0.5658075742952917 1104 94-95 0.2989554844526552 1104 96-97 0.5081498906412385 1104 98-99 0.8293179827667672 1104 100-101 0.6754304078120832 1105 1 0.2757938924375978 1105 2 0.1998936487355749 1105 3 0.08953815486417938 1105 4 0.07762595666675054 1105 5 0.26632547095214676 1105 6 0.29434866743002175 1105 7 0.21587575769458311 1105 8 0.20228114228600447 1105 9 0.3419293256903231 1105 10-11 0.2817974840842865 1105 12-13 0.18528798813530045 1105 14-15 0.2575896338121595 1105 16-17 0.10364368729001683 1105 18-19 0.27037257295113903 1105 20-21 0.4000287461680969 1105 22-23 0.40678571081584636 1105 24-25 0.23789201974820173 1105 26-27 0.5567455551184253 1105 28-29 0.6151032415782964 1105 30-31 0.7908922926671096 1105 32-33 1.1902402028085746 1105 34-35 1.0888392924697214 1105 36-37 1.0287811717044377 1105 38-39 0.8490627303767866 1105 40-41 1.0062520675988438 1105 42-43 1.0058492677315343 1105 44-45 0.9967918740345141 1105 46-47 1.1157106386337503 1105 48-49 1.1394316682501326 1105 50-51 0.9075228865698932 1105 52-53 0.8378286326268878 1105 54-55 0.9470063210798472 1105 56-57 0.9058823744295879 1105 58-59 1.112888251955816 1105 60-61 1.305770783117282 1105 62-63 1.551907989302677 1105 64-65 1.5460990203210159 1105 66-67 1.4761950490417277 1105 68-69 1.2651401291748456 1105 70-71 1.3314418740800633 1105 72-73 1.0225724594571552 1105 74-75 0.941634670380413 1105 76-77 1.0051635938745207 1105 78-79 0.8219186483932681 1105 80-81 0.7663913675586258 1105 82-83 0.499405877125767 1105 84-85 0.9697017772064278 1105 86-87 0.7144678628090269 1105 88-89 0.5960280628180286 1105 90-91 0.6865226138796636 1105 92-93 0.8523232537378007 1105 94-95 1.1537464252192073 1105 96-97 0.9884809010941993 1105 98-99 1.0025584008852313 1105 100-101 1.1292631604253245 1106 1 0.12758876423247045 1106 2 0.1864321102740405 1106 3 0.19376892409495383 1106 4 0.14929262333342308 1106 5 0.009274188900867841 1106 6 -0.08975389667254063 1106 7 -0.07694475512592902 1106 8 0.0270247320295951 1106 9 -0.2607629820019781 1106 10-11 0.11423338152018658 1106 12-13 0.05631362916094673 1106 14-15 -0.01798728926476656 1106 16-17 0.1346693283156597 1106 18-19 0.019090521669085092 1106 20-21 -0.10176612562677434 1106 22-23 -0.06571428918415734 1106 24-25 -0.1698002879441134 1106 26-27 -0.30979290642003576 1106 28-29 -0.3163711173960593 1106 30-31 -0.37346668169186614 1106 32-33 -0.06084954078117022 1106 34-35 -0.10731455368412668 1106 36-37 -0.5884624180391498 1106 38-39 -0.48555265423859595 1106 40-41 -0.2655428041960377 1106 42-43 -0.1357532963710213 1106 44-45 -0.2464132541706192 1106 46-47 -0.47102013059701875 1106 48-49 -0.5114016650831985 1106 50-51 -0.6703617288147186 1106 52-53 -0.7233893160910654 1106 54-55 -0.5974167558432342 1106 56-57 -0.9037971127498992 1106 58-59 -0.5507014916339301 1106 60-61 -0.47794716560066774 1106 62-63 -0.41860483121014624 1106 64-65 -0.28095226173027044 1106 66-67 -0.4045100791633942 1106 68-69 -0.5263342297995237 1106 70-71 -0.6128529977148105 1106 72-73 -0.6214018995172026 1106 74-75 -0.05855763731189967 1106 76-77 -0.006182559971634305 1106 78-79 -0.2364787875041685 1106 80-81 -0.06251888885163126 1106 82-83 -0.0855300203101308 1106 84-85 -0.27081104330639505 1106 86-87 -0.3446988038576393 1106 88-89 -0.20807450128453553 1106 90-91 -0.22828507842802637 1106 92-93 -0.28062546421091383 1106 94-95 -0.01035613888335618 1106 96-97 -0.17651909890579986 1106 98-99 -0.20167236834553393 1106 100-101 0.4081093142714778 1107 1 0.18074213260322836 1107 2 0.24533878331114778 1107 3 0.24777832049565518 1107 4 0.23443755086965012 1107 5 0.2061805434159183 1107 6 0.2678269282995842 1107 7 0.1392919067629066 1107 8 0.25431012779325357 1107 9 0.3261736321085422 1107 10-11 0.1414869250780768 1107 12-13 0.2822652138081807 1107 14-15 0.38891468557199005 1107 16-17 0.3293062131699358 1107 18-19 0.24066242802359739 1107 20-21 0.3204945846774123 1107 22-23 0.34797618700631716 1107 24-25 0.27194999076269255 1107 26-27 0.21799814311014387 1107 28-29 0.3424220821580093 1107 30-31 0.3863477792095509 1107 32-33 1.0120621489783446 1107 34-35 0.8508993338775852 1107 36-37 0.5060689563835297 1107 38-39 0.4070855047039075 1107 40-41 0.45330175703983144 1107 42-43 0.6645035120379532 1107 44-45 0.6988208595417547 1107 46-47 0.8423358974329247 1107 48-49 1.0544627241507527 1107 50-51 0.9982061163835638 1107 52-53 1.0357582392521394 1107 54-55 1.2656088055518993 1107 56-57 1.0206235752577442 1107 58-59 1.051066305786044 1107 60-61 1.3097976982311508 1107 62-63 1.471007368184658 1107 64-65 1.4040493308800208 1107 66-67 1.3211329372404848 1107 68-69 1.1730904397338477 1107 70-71 1.2489781059641203 1107 72-73 1.237313660285313 1107 74-75 1.5699162438793728 1107 76-77 1.1506397843507123 1107 78-79 1.1384714434243186 1107 80-81 1.1953147630037613 1107 82-83 1.391486622467383 1107 84-85 1.2629522948047693 1107 86-87 1.4181117551485727 1107 88-89 1.153388311265232 1107 90-91 1.2739346221612387 1107 92-93 1.7160499618123382 1107 94-95 1.6321625742875305 1107 96-97 1.590561646435816 1107 98-99 1.531067717655418 1107 100-101 1.5624929740899205 1108 1 0.1909790776227851 1108 2 0.10298006848866237 1108 3 0.17916778449381354 1108 4 0.11997163567910007 1108 5 0.16323905119906357 1108 6 0.026817803232489723 1108 7 0.062418967571126416 1108 8 0.12709595710082056 1108 9 0.07316389359156261 1108 10-11 0.1010567433435483 1108 12-13 0.0032509510982663414 1108 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2316 100-101 -0.23832158533738834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 1.0 12 2.0 13 25.0 14 104.0 15 394.0 16 1037.0 17 2078.0 18 2939.0 19 4182.0 20 5375.0 21 7444.0 22 7861.0 23 5432.0 24 4397.0 25 4434.0 26 4808.0 27 6027.0 28 7545.0 29 8973.0 30 5875.0 31 4382.0 32 4764.0 33 5795.0 34 7412.0 35 13057.0 36 18071.0 37 43335.0 38 112520.0 39 43366.0 40 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.246163408908494 24.502284152148068 16.877681691177802 21.373870747765636 2 12.899172198901287 25.35174766380487 43.96506781821524 17.784012319078602 3 13.181534641381617 34.44399912006582 36.321694295193815 16.052771943358756 4 10.48387339796229 24.862058262491598 40.40525188116067 24.24881645838544 5 7.6698920875233165 41.886373978055886 39.05581856480323 11.38791536961756 6 18.644358928770533 45.927140244031065 20.313823103093934 15.114677724104473 7 19.24283471400632 36.977124724643886 26.376027217690297 17.404013343659503 8 20.75047537540343 37.9266701422058 24.797569935180192 16.52528454721058 9 23.386360417426314 17.911783584405878 23.14166639043162 35.560189607736184 10-11 19.143691444448127 30.703676136004077 32.30895333033995 17.843679089207846 12-13 22.082279873313585 29.532037740738843 30.55074236913239 17.83494001681518 14-15 19.557591993804298 31.085031174380656 28.28039163098212 21.076985200832922 16-17 17.411245679432742 34.116735926326605 30.1604975847012 18.31152080953945 18-19 17.35640046648566 30.24397079341737 34.144007859138206 18.255620880958766 20-21 16.481137164261416 30.186865475541147 34.267258914607204 19.064738445590233 22-23 17.398287744505687 31.579994153861918 33.933215406080585 17.088502695551814 24-25 17.973559785802323 32.816572897424386 33.624183725436424 15.585683591336865 26-27 16.946568106001937 33.028721413439484 34.90084166307561 15.123868817482967 28-29 16.19982943741468 29.713750177041554 37.36857489837062 16.717845487173154 30-31 18.116398417323857 32.0311110977179 34.46177861217504 15.390711872783214 32-33 16.62849600564122 31.48943928303445 35.183204105555944 16.698860605768388 34-35 15.276199889706879 32.434615164400036 37.35847976302046 14.930705182872622 36-37 17.57201447672544 31.517765931479648 34.242096413062804 16.66812317873211 38-39 14.620016091947097 31.63905822934339 36.09568380227999 17.645241876429516 40-41 14.87887344316439 30.1665245311789 37.86082575193691 17.0937762737198 42-43 16.996918718970598 29.787625153499025 35.95124192952982 17.26421419800056 44-45 14.746431294316892 32.152554069243585 35.962337611460846 17.13867702497868 46-47 17.566242004625828 29.487919356904463 34.58732945085246 18.358509187617248 48-49 16.900816359740716 29.701903633991183 33.610425537685444 19.786854468582657 50-51 14.608888252710248 30.02689528918086 34.853733322283915 20.510483135824977 52-53 15.378808653489754 29.91505018939679 37.18610909375819 17.520032063355263 54-55 15.745849693981793 29.770704821255833 34.19327814659342 20.290167338168956 56-57 16.80177071687515 31.573967207384214 31.242183803786734 20.382078271953905 58-59 14.768128301636619 31.32731442278427 35.17898524302155 18.725572032557565 60-61 17.532085956310663 33.78480184906718 30.415588094369927 18.267524100252228 62-63 14.258700650608871 36.06359031228623 31.18537983323439 18.492329203870504 64-65 15.16846822141796 34.647408563688245 31.312397730251956 18.871725484641832 66-67 15.353495478283405 34.80968409760037 30.213082692719144 19.623737731397075 68-69 14.856870775359896 34.855936570503836 30.484912591574677 19.80228006256159 70-71 15.901344913106499 33.08145719511938 31.74061227749267 19.27658561428145 72-73 16.157791485732712 32.31347354019823 30.79151888091658 20.737216093152487 74-75 15.376247201236728 32.91586684064452 30.30258284791302 21.405303110205733 76-77 16.02504798956133 31.73458533101497 31.78189686086493 20.45846981855878 78-79 16.224539917973257 31.437306196002325 30.981819715950014 21.356334170074405 80-81 15.639022067664529 31.88269754070449 30.672336014320024 21.805944377310958 82-83 16.700819363373643 31.135205503807523 30.656665953478 21.507309179340833 84-85 16.269892690217965 30.516388774209492 30.343867431285275 22.869851104287267 86-87 15.562955317658226 31.778328574549676 30.54476144556529 22.11395466222681 88-89 17.068213185793237 29.943768419910683 30.63973216169141 22.34828623260467 90-91 16.735374063560368 29.6113813200258 30.618035088988133 23.0352095274257 92-93 16.520915011014246 30.50343083928243 30.204042273002596 22.771611876700728 94-95 17.150278896948258 28.761944654550497 30.92953595525595 23.158240493245298 96-97 17.593259463059336 28.7113183041378 30.170291372727466 23.525130860075397 98-99 16.41154374106291 29.734188983330494 30.28838257740704 23.56588469819955 100-101 16.768371694963268 28.93214240512051 31.202801333164576 23.096684566751648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2671.0 1 1555.5 2 487.5 3 481.5 4 344.5 5 315.0 6 504.0 7 911.5 8 1380.0 9 1537.5 10 1136.0 11 734.5 12 695.0 13 711.0 14 711.0 15 664.0 16 652.5 17 687.5 18 743.5 19 823.5 20 881.5 21 994.5 22 1145.5 23 1345.5 24 1575.5 25 1913.0 26 2587.5 27 3564.0 28 4325.0 29 5121.5 30 6858.5 31 8489.0 32 11370.0 33 17490.5 34 23071.5 35 22802.0 36 22116.5 37 23064.0 38 20802.0 39 18691.0 40 16947.5 41 14723.0 42 12743.5 43 11533.5 44 11138.0 45 10560.5 46 8942.0 47 6847.0 48 5775.5 49 4774.5 50 4067.5 51 3322.5 52 2039.5 53 1094.0 54 653.0 55 465.0 56 336.0 57 269.0 58 204.0 59 160.5 60 147.5 61 137.5 62 118.0 63 87.5 64 64.0 65 35.0 66 12.5 67 8.0 68 3.0 69 1.0 70 0.5 71 1.0 72 1.5 73 0.5 74 1.0 75 3.0 76 2.0 77 0.5 78 1.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1292780019467037 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 1.5067366194254511E-4 44-45 0.0 46-47 0.0031641469007934477 48-49 0.001205389295540361 50-51 1.5067366194254511E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0030134732388509026 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.5067366194254511E-4 88-89 3.0134732388509023E-4 90-91 3.0134732388509023E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.002862799576908357 100-101 3.0134732388509023E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 331843.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.09280593533689 #Duplication Level Percentage of deduplicated Percentage of total 1 71.0665047503393 14.989919932016043 2 9.532109436388314 4.021178689922644 3 4.24744624616044 2.68771678173112 4 2.557325523251661 2.157646839017246 5 1.725837559825702 1.8201378362659453 6 1.3386670476462605 1.6941746548819774 7 1.028644903207372 1.5187905123808547 8 0.7943424530323595 1.3403928966408813 9 0.6629044931780842 1.2584264245441368 >10 5.771840845774698 25.402374014217564 >50 0.8114865347524823 11.919190701626974 >100 0.43431673690977923 16.041320745051124 >500 0.011429387813415244 1.5654993475830437 >1k 0.014286734766769057 8.600452623680475 >5k 0.0014286734766769055 1.6571089340441114 >10k+ 0.0014286734766769055 3.325669066395856 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 11036 3.325669066395856 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 5499 1.6571089340441114 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 1.429893051834753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4179 1.259330466515792 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 3810 1.148133304002194 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 3228 0.9727491615010712 TruSeq Adapter, Index 1 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2693 0.8115283432225481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2620 0.7895299885789363 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2418 0.7286578291541482 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 2134 0.6430751891707825 RNA PCR Primer, Index 1 (95% over 23bp) CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 1405 0.42339299005855174 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 1308 0.3941622996416981 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 812 0.24469402699469328 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 809 0.243789985023038 TruSeq Adapter, Index 1 (96% over 26bp) AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG 695 0.20943639010013773 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 643 0.19376632925811302 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 599 0.18050704700716905 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 569 0.17146662729061635 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 549 0.16543968081291455 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 519 0.15639926109636185 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 500 0.1506736619425451 TruSeq Adapter, Index 1 (96% over 28bp) GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 468 0.14103054757822223 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 462 0.1392224636349117 TruSeq Adapter, Index 1 (95% over 24bp) ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 447 0.13470225377663533 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.1304833912422441 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC 426 0.12837395997504844 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 424 0.12777126532727826 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 424 0.12777126532727826 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 413 0.12445644476454228 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 411 0.12385375011677209 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 410 0.123552402792887 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 399 0.12023758223015099 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 383 0.11541602504798958 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 381 0.11481333040021938 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 365 0.10999177321805793 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 358 0.1078823419508623 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 352 0.10607425800755177 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 343 0.10336213209258595 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 341 0.10275943744481578 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 335 0.10095135350150523 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0134732388509023E-4 0.0 10-11 0.0 0.0 0.0 4.5202098582763537E-4 0.0 12-13 0.0 0.0 0.0 6.026946477701805E-4 0.0 14-15 0.0 0.0 0.0 6.026946477701805E-4 0.0 16-17 0.0 0.0 0.0 6.026946477701805E-4 0.0 18-19 0.0 0.0 0.0 6.026946477701805E-4 0.0 20-21 0.0 0.0 0.0 0.0019587576052530866 0.0 22-23 0.0 0.0 0.0 0.01084850365986325 0.0 24-25 0.0 0.0 0.0 0.029984058726566478 0.0 26-27 0.0 0.0 0.0 0.06855651618385802 0.0 28-29 0.0 0.0 0.0 0.126867223355623 0.0 30-31 0.0 0.0 0.0 0.22992800812432385 0.0 32-33 0.0 0.0 0.0 0.3760814602085926 0.0 34-35 0.0 0.0 0.0 0.632980053820632 0.0 36-37 0.0 0.0 0.0 1.0325666052922617 0.0 38-39 0.0 0.0 0.0 1.445864460000663 0.0 40-41 0.0 0.0 0.0 1.8040157544380926 0.0 42-43 0.0 0.0 0.0 2.135045789725864 0.0 44-45 0.0 0.0 0.0 2.502237503879847 0.0 46-47 0.0 0.0 0.0 2.980927727871313 0.0 48-49 0.0 0.0 0.0 3.4123064220128194 0.0 50-51 0.0 0.0 0.0 3.788086534897527 0.0 52-53 0.0 0.0 0.0 4.1706469625696485 0.0 54-55 0.0 0.0 0.0 4.574301702913727 0.0 56-57 0.0 0.0 0.0 5.079058470421254 0.0 58-59 0.0 0.0 0.0 5.577034923141365 0.0 60-61 0.0 0.0 0.0 6.0317680348839655 0.0 62-63 0.0 0.0 0.0 6.458023824519426 0.0 64-65 0.0 0.0 0.0 6.960068466111986 0.0 66-67 0.0 0.0 0.0 7.502192301781264 0.0 68-69 0.0 0.0 0.0 8.090422278004961 0.0 70-71 0.0 0.0 0.0 8.713909891123212 0.0 72-73 0.0 0.0 0.0 9.303646603966333 0.0 74-75 0.0 0.0 0.0 9.893986011457226 0.0 76-77 0.0 0.0 0.0 10.572620184846448 0.0 78-79 0.0 0.0 0.0 11.308811697097724 0.0 80-81 0.0 0.0 0.0 11.979610840065934 0.0 82-83 0.0 0.0 0.0 12.635643964163776 0.0 84-85 0.0 0.0 0.0 13.33085224036668 0.0 86-87 0.0 0.0 0.0 14.065235668674644 0.0 88-89 0.0 0.0 0.0 14.79961909698261 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGAGAG 25 0.0050186105 56.99864 5 CCTTTAG 70 7.7601726E-8 47.60648 1 CTTTAGG 75 1.3591307E-7 44.332275 2 GGTATCA 2490 0.0 41.488377 1 TGGGAAC 70 4.3016153E-6 40.713318 6 TTAGGCA 95 1.9215804E-8 39.999046 4 CTATACA 85 3.5670564E-7 39.205334 1 GATCCTC 50 0.0015964957 38.085182 1 GTACCCA 105 4.5498382E-8 36.271606 1 CATTCCG 95 8.6767614E-7 34.999165 9 GGGGCAG 110 6.988557E-8 34.544636 7 ACCCATG 100 1.2957444E-6 33.249207 3 GTATCAA 3800 0.0 32.823418 1 CATATAG 75 3.074194E-4 31.665913 3 GGCATTC 120 1.500648E-7 31.665913 7 GGATATG 80 4.4283556E-4 29.75405 1 CTTTACA 195 1.6370905E-11 29.296293 1 CAGTACA 65 0.0058388407 29.230072 9 AGGCATT 115 3.8543967E-6 28.912355 6 TGGGGCA 150 3.322384E-8 28.499321 6 >>END_MODULE