FastQCFastQC Report
Fri 10 Feb 2017
SRR2031571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268966
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.13010331578348905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42800.045.5022621
GGTATCA23700.044.0927541
CAACGCA50200.038.2270285
TCAACGC50000.038.0949364
ATCAACG49950.038.0379753
AACGCAG51800.036.954576
TATCAAC55150.035.0543442
ACGCAGA59550.032.1452037
CGCAGAG61400.031.0992978
TACATGG58150.026.8744182
GTACATG61650.026.8126091
ACATGGG59750.025.6777823
GCAGAGT74200.024.7102039
CATGGGG39600.022.4305214
AGAGTAC64000.021.70894810-11
CAGAGTA69700.019.83138710-11
GTATAAA18700.018.796761
GAGTACT40800.018.74384312-13
CGTATAC1300.007565412718.2692013
GTATAAT15150.017.8712581
Produced by FastQC (version 0.11.5)