FastQCFastQC Report
Fri 10 Feb 2017
SRR2031571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268966
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT47420.20899387650586215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.16201212358404665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28200.1242856878419509No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.049.7431341
GTATCAA44500.042.542951
TCAACGC51400.036.0398944
ATCAACG52200.035.4875563
CAACGCA53700.034.584745
AACGCAG54400.034.1397136
TATCAAC56600.033.1484072
ACGCAGA61550.030.0966827
CGCAGAG62350.029.4819728
GTACATG57650.024.5053941
TACATGG54900.024.3982562
GCAGAGT77900.023.3530359
ACATGGG55850.022.707543
AGAGTAC65950.021.06653410-11
GAGTACT43200.019.9010912-13
CAGAGTA71550.019.4177210-11
CATGGGG36050.019.3684144
TATACCG2152.3966431E-517.6739465
TAGGCGG1350.00940458717.5921235
TCTATAC9150.016.6115783
Produced by FastQC (version 0.11.5)