FastQCFastQC Report
Fri 10 Feb 2017
SRR2031569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214891
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA78770.6483709238112719No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT64190.5283601574132988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.46481536203659424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.35994998728280975No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT25450.2094838137742398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.20915456612980096No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC17580.14470433973088945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.060.815111
GTATCAA51700.045.2030941
GCGTATC459.625294E-442.222221
ATCAACG61700.037.3379253
TCAACGC62250.037.0843354
CAACGCA63300.036.4691935
AACGCAG63000.036.4166686
TATCAAC64100.036.0881422
ACGCAGA69050.032.881977
CGCAGAG70200.032.3433048
GTACATG59950.029.7914921
GCAGAGT76300.029.384019
ACGATGC650.005844202429.230778
ACATGGG58250.028.8669513
GGTCGCG504.9553506E-528.594-95
TACATGG61400.028.3916932
CATGGGG21900.027.3287684
GTTCGCA1301.0021688E-525.5769239
CATGGGA37150.025.3162864
GAGTACT45250.025.08839812-13
Produced by FastQC (version 0.11.5)