FastQCFastQC Report
Fri 10 Feb 2017
SRR2031569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214891
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA81930.6743814877219437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.556922390568372No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT64860.5338750554576501No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT62340.5131324538580004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53670.4417680269258724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.2577185936845363No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT25210.20750832790760654No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT19480.1603436028417364TruSeq Adapter, Index 1 (95% over 21bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC17860.14700907324196164No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA12840.1056884938648817No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT12380.10190214595383455RNA PCR Primer, Index 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29500.057.246831
GTATCAA55150.042.0939251
TCAACGC66850.034.460664
ATCAACG67200.034.2811783
AACGCAG67450.034.1541186
CAACGCA67400.034.0385065
TATCAAC68600.033.5815622
GTACATG58800.030.662461
ACGCAGA75000.030.5892727
ACATGGG56450.030.5440033
CGCAGAG75250.030.4876448
CATGGGA35650.030.244724
CTTCGGC650.00584488229.2300729
TACATGG62050.029.0122012
GCAGAGT82200.027.4476439
ACGCAGT700.00839273127.142218
TAGCCCG700.00839273127.142214
GAGTACT51850.024.8258312-13
CAACGCG601.7118572E-423.74943434-35
ACCGTGA1401.7799179E-523.7494348
Produced by FastQC (version 0.11.5)