##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031569_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1214891 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7661979552075 34.0 31.0 34.0 31.0 34.0 2 32.91122084203439 34.0 33.0 34.0 31.0 34.0 3 33.01363990678999 34.0 33.0 34.0 31.0 34.0 4 36.38640009679881 37.0 37.0 37.0 35.0 37.0 5 36.324686741444296 37.0 37.0 37.0 35.0 37.0 6 36.34059516450447 37.0 37.0 37.0 35.0 37.0 7 36.36875653865244 37.0 37.0 37.0 35.0 37.0 8 36.399527200382586 37.0 37.0 37.0 35.0 37.0 9 38.251878563591305 39.0 39.0 39.0 37.0 39.0 10-11 38.222749201368686 39.0 39.0 39.0 37.0 39.0 12-13 38.23496264273915 39.0 39.0 39.0 37.0 39.0 14-15 39.783922590586315 41.0 40.0 41.0 37.5 41.0 16-17 39.77874764073485 41.0 40.0 41.0 37.5 41.0 18-19 39.82227129841278 41.0 40.0 41.0 38.0 41.0 20-21 39.81810837350841 41.0 40.0 41.0 38.0 41.0 22-23 39.78975438948844 41.0 40.0 41.0 37.5 41.0 24-25 39.74032485218839 41.0 40.0 41.0 37.0 41.0 26-27 39.63385974544218 41.0 40.0 41.0 37.0 41.0 28-29 39.549815580163155 41.0 40.0 41.0 37.0 41.0 30-31 39.39696771150663 41.0 39.0 41.0 36.0 41.0 32-33 38.51595163681351 40.0 38.0 41.0 35.0 41.0 34-35 38.68810905669727 40.0 38.0 41.0 35.0 41.0 36-37 38.8704521640213 40.0 38.5 41.0 35.0 41.0 38-39 38.80678925105215 40.0 38.5 41.0 35.0 41.0 40-41 38.72228125815403 40.0 38.5 41.0 35.0 41.0 42-43 38.518590968243245 40.0 38.0 41.0 35.0 41.0 44-45 38.3390283572765 40.0 38.0 41.0 34.5 41.0 46-47 38.06664054635354 40.0 38.0 41.0 33.5 41.0 48-49 37.859669303665925 40.0 38.0 41.0 33.0 41.0 50-51 37.85743288904108 40.0 38.0 41.0 33.0 41.0 52-53 37.98876689349086 41.0 39.0 41.0 33.0 41.0 54-55 37.9236643451964 41.0 39.0 41.0 33.0 41.0 56-57 37.80538871388462 41.0 38.0 41.0 33.0 41.0 58-59 37.69138424764032 40.5 38.0 41.0 33.0 41.0 60-61 37.50728172321632 40.0 38.0 41.0 32.5 41.0 62-63 37.24702709955049 40.0 37.0 41.0 31.5 41.0 64-65 37.04226593167618 40.0 37.0 41.0 31.0 41.0 66-67 36.763275059243995 39.5 36.0 41.0 31.0 41.0 68-69 36.39929755015059 39.0 36.0 41.0 30.5 41.0 70-71 36.00892096492607 39.0 35.0 41.0 29.0 41.0 72-73 35.635557017049265 37.5 35.0 40.0 29.0 41.0 74-75 35.20640864077518 37.0 35.0 39.5 29.0 41.0 76-77 33.66407315553412 35.5 33.5 38.5 26.5 39.5 78-79 34.331668437744625 36.0 35.0 39.0 28.0 40.0 80-81 34.13158135174267 36.0 35.0 37.5 28.0 39.5 82-83 33.80392685434331 35.5 35.0 37.0 28.0 39.0 84-85 33.446285716167125 35.0 35.0 37.0 27.0 39.0 86-87 33.16163960388216 35.0 34.0 36.0 26.5 37.5 88-89 32.897957512237724 35.0 34.0 36.0 26.0 37.0 90-91 32.676565634283236 35.0 34.0 36.0 26.0 37.0 92-93 32.50979223650517 35.0 34.0 35.0 25.5 36.0 94-95 32.377135479643854 35.0 34.0 35.0 25.0 36.0 96-97 32.2538367639566 35.0 34.0 35.0 25.0 36.0 98-99 32.17645080916724 35.0 34.0 35.0 25.0 36.0 100-101 31.084466836942575 34.5 31.5 35.0 21.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42849579809924876 1101 2 0.35095666771905343 1101 3 0.3978667812035823 1101 4 0.24853479624437114 1101 5 0.2636682595007116 1101 6 0.282785354864302 1101 7 0.17796744561265854 1101 8 0.2470613453111028 1101 9 0.2453110881534215 1101 10-11 0.3778138673724811 1101 12-13 0.3448271843146813 1101 14-15 0.4569671090590859 1101 16-17 0.41913455234659125 1101 18-19 0.4790439961935604 1101 20-21 0.41431343656709174 1101 22-23 0.3961381780159172 1101 24-25 0.40620273601189183 1101 26-27 0.3455587368147377 1101 28-29 0.3726692872292148 1101 30-31 0.3978458969589127 1101 32-33 0.582903496625832 1101 34-35 0.14118763243995858 1101 36-37 0.28781010272578555 1101 38-39 0.5003304510106332 1101 40-41 0.5068177920876167 1101 42-43 0.4313631479371125 1101 44-45 0.5692109349635643 1101 46-47 0.6497734017123804 1101 48-49 0.6028166914797879 1101 50-51 0.686405530885061 1101 52-53 0.6794545875915787 1101 54-55 0.6764182353016466 1101 56-57 0.6832610265129944 1101 58-59 0.7046188849827146 1101 60-61 0.6271675174345503 1101 62-63 0.6762678391090589 1101 64-65 0.7010267031305091 1101 66-67 0.6425624646571322 1101 68-69 0.34055602310234434 1101 70-71 0.2936096629887004 1101 72-73 0.42020376258542314 1101 74-75 0.4544134461411389 1101 76-77 0.5584326959169346 1101 78-79 0.5280348197050628 1101 80-81 0.4237475458682596 1101 82-83 0.45512748875960085 1101 84-85 0.4035670226683763 1101 86-87 0.3965324109420081 1101 88-89 0.3453854390779938 1101 90-91 0.42330100340127075 1101 92-93 0.47550661521613335 1101 94-95 0.4421519540620906 1101 96-97 0.5123452090800384 1101 98-99 0.3564809394662163 1101 100-101 0.40665797302746753 1102 1 0.10997727958072545 1102 2 0.1748117055525853 1102 3 0.06513081943536037 1102 4 0.05673869907192852 1102 5 -0.039159299240822065 1102 6 0.11126006613471873 1102 7 0.05005028113635035 1102 8 0.046264849890945925 1102 9 0.16363048281690595 1102 10-11 0.16003210711760119 1102 12-13 0.23076904014900634 1102 14-15 0.3422319437862882 1102 16-17 0.25167139025978 1102 18-19 0.27247290897731347 1102 20-21 0.289184006061312 1102 22-23 0.2566917423329258 1102 24-25 0.3560235245423584 1102 26-27 0.3418749454965422 1102 28-29 0.41301178025987895 1102 30-31 0.48910435972275224 1102 32-33 0.4166151652837442 1102 34-35 0.21251379731450726 1102 36-37 0.294620138568078 1102 38-39 0.40403415471433135 1102 40-41 0.4151610816136966 1102 42-43 0.5452819054042592 1102 44-45 0.4117437903996475 1102 46-47 0.49949462831532543 1102 48-49 0.4723507416589996 1102 50-51 0.5254138940869666 1102 52-53 0.4549225286509184 1102 54-55 0.43000246469232906 1102 56-57 0.3297763590498306 1102 58-59 0.3235555635968126 1102 60-61 0.38196640234096435 1102 62-63 0.4559173811241948 1102 64-65 0.43499723279996516 1102 66-67 0.6180104933309636 1102 68-69 0.42283400000397364 1102 70-71 0.3335539083092911 1102 72-73 0.342923794445241 1102 74-75 0.36952694673850317 1102 76-77 0.32480067680104696 1102 78-79 0.40577277271183476 1102 80-81 0.401525323646041 1102 82-83 0.39578857995832095 1102 84-85 0.4402058119953338 1102 86-87 0.3425698462267519 1102 88-89 0.4800130774690743 1102 90-91 0.4811265709042516 1102 92-93 0.4198514977330632 1102 94-95 0.5269388915372062 1102 96-97 0.528056081242525 1102 98-99 0.5784642130623894 1102 100-101 0.5273668539513992 1103 1 0.40365316890878944 1103 2 0.40569487100798085 1103 3 0.3541103765776157 1103 4 0.20710845554266655 1103 5 0.21234097701400856 1103 6 0.2616000720848177 1103 7 0.2098637028712318 1103 8 0.23331274822916725 1103 9 0.1504616273619348 1103 10-11 0.2664709697976946 1103 12-13 0.2927606250017405 1103 14-15 0.3915179312910695 1103 16-17 0.37775090915135223 1103 18-19 0.3464316407551138 1103 20-21 0.34663544896156395 1103 22-23 0.3331325175464812 1103 24-25 0.35664191036411097 1103 26-27 0.4437715411869618 1103 28-29 0.40996128937629095 1103 30-31 0.4343315261981644 1103 32-33 0.7527822357074712 1103 34-35 0.498444988610359 1103 36-37 0.5437169977631129 1103 38-39 0.6166164999053692 1103 40-41 0.6886554927658892 1103 42-43 0.6777875367528097 1103 44-45 0.47469695458001837 1103 46-47 0.5529488762646722 1103 48-49 0.6624895065873844 1103 50-51 0.7086972890463841 1103 52-53 0.7313271782322843 1103 54-55 0.8149667640675702 1103 56-57 0.8553490565720381 1103 58-59 0.9003735329112743 1103 60-61 0.9155611152484369 1103 62-63 1.0474384827434733 1103 64-65 0.987618466171547 1103 66-67 0.9327030004498411 1103 68-69 1.0079534270939519 1103 70-71 1.003902201904431 1103 72-73 0.9250883573250874 1103 74-75 0.9792341166139735 1103 76-77 0.9442107664776174 1103 78-79 0.9678557341636562 1103 80-81 0.9497466675195554 1103 82-83 0.9625763737447102 1103 84-85 0.954414873153901 1103 86-87 0.9142579757690257 1103 88-89 0.9150836287037194 1103 90-91 0.8926283346851207 1103 92-93 0.933293420466704 1103 94-95 0.7947382030251546 1103 96-97 0.8465056541100466 1103 98-99 0.8510937340790079 1103 100-101 0.9448527224540975 1104 1 0.3447021372657346 1104 2 0.3132051274374632 1104 3 0.23027945953656115 1104 4 0.15616943910988113 1104 5 0.15198554579396273 1104 6 0.23010889598923967 1104 7 0.2005879155449506 1104 8 0.12545523572270412 1104 9 0.18077153278527902 1104 10-11 0.2054780274212078 1104 12-13 0.20572160181883703 1104 14-15 0.1376778640898948 1104 16-17 0.20002686148051652 1104 18-19 0.18341535047003532 1104 20-21 0.22947496115303068 1104 22-23 0.2545095791450578 1104 24-25 0.1964409818478643 1104 26-27 0.22318424340924992 1104 28-29 0.2484956512276284 1104 30-31 0.1885034742072591 1104 32-33 0.5576335081047432 1104 34-35 0.46121714962317384 1104 36-37 0.1899395566578974 1104 38-39 0.21516634952774183 1104 40-41 0.2600061164018115 1104 42-43 0.35815350565726334 1104 44-45 0.48979565630730093 1104 46-47 0.2521195492514394 1104 48-49 0.3425910958651954 1104 50-51 0.4203538055354272 1104 52-53 0.3828795178982247 1104 54-55 0.31017324268094626 1104 56-57 0.29928932552674326 1104 58-59 0.26334050983336965 1104 60-61 0.30751984952628675 1104 62-63 0.4297314228699207 1104 64-65 0.4272489722054047 1104 66-67 0.4061825363417242 1104 68-69 0.2938649930463555 1104 70-71 0.12450900002340148 1104 72-73 0.08976123910051825 1104 74-75 0.33027963491054635 1104 76-77 0.18953818470743755 1104 78-79 0.195304714520816 1104 80-81 0.26594657601796 1104 82-83 0.10439073049595748 1104 84-85 0.03969980314017363 1104 86-87 0.11439147810531125 1104 88-89 0.12966519638116125 1104 90-91 0.00905193459811926 1104 92-93 0.01510766471597691 1104 94-95 0.16906412872885568 1104 96-97 0.1924773336144412 1104 98-99 0.039122024574091085 1104 100-101 -0.0242776748278537 1105 1 0.1767975560784194 1105 2 0.1216627040164866 1105 3 0.17096799301447163 1105 4 0.020333169118011085 1105 5 0.10077925682676181 1105 6 0.11709723971382147 1105 7 0.05312247775693635 1105 8 0.05901446586636894 1105 9 0.10402986837758021 1105 10-11 0.1432118306199044 1105 12-13 0.08560774982642982 1105 14-15 0.056287243325463976 1105 16-17 0.11142561453012689 1105 18-19 0.15949287825534242 1105 20-21 0.25930689392613715 1105 22-23 0.22212953035135286 1105 24-25 0.21040140472668867 1105 26-27 0.14970904687902475 1105 28-29 0.17291961436126257 1105 30-31 0.27673876832490407 1105 32-33 0.6241420470041703 1105 34-35 0.39722961912710986 1105 36-37 0.27580293426700564 1105 38-39 0.21294352491403146 1105 40-41 0.11777244874119219 1105 42-43 0.23591170571148012 1105 44-45 0.14500031881746622 1105 46-47 0.0509539217101036 1105 48-49 0.10130619480799652 1105 50-51 0.13701143632967216 1105 52-53 0.11628965614708164 1105 54-55 0.20972596699647994 1105 56-57 0.053816297605898455 1105 58-59 -0.010853038553726435 1105 60-61 0.009769781757242413 1105 62-63 0.1331063212163599 1105 64-65 0.06487434493514144 1105 66-67 0.09696594647996903 1105 68-69 0.052634307223641486 1105 70-71 0.03869983764877105 1105 72-73 0.15751672839300568 1105 74-75 0.15378193905801396 1105 76-77 0.26896350322193996 1105 78-79 0.16483575274255458 1105 80-81 0.2006036646598588 1105 82-83 0.14333082266185215 1105 84-85 0.2552595754361917 1105 86-87 0.1377311365493341 1105 88-89 0.1105814338438833 1105 90-91 0.052478337417305454 1105 92-93 0.021925230451948607 1105 94-95 0.14606331550034213 1105 96-97 0.17390861580473427 1105 98-99 0.048663905842722954 1105 100-101 0.05509342845217091 1106 1 0.19691107749013526 1106 2 0.12703530152126064 1106 3 0.12023740967177332 1106 4 0.07105813258884552 1106 5 0.05078487669862852 1106 6 0.1435050176164907 1106 7 0.0911971096553259 1106 8 0.030667832169619658 1106 9 -0.07467643711865435 1106 10-11 0.1225981406120411 1106 12-13 0.12127145669391126 1106 14-15 0.1112074411449484 1106 16-17 0.10253778717258655 1106 18-19 0.05108554994242809 1106 20-21 0.16720843377103023 1106 22-23 0.1669817277212573 1106 24-25 0.1306413732554077 1106 26-27 0.12207613690937791 1106 28-29 0.07048620984288334 1106 30-31 0.04047935503952971 1106 32-33 0.4861404734400452 1106 34-35 0.22303045086814421 1106 36-37 8.085541388709316E-4 1106 38-39 -0.050350281987171286 1106 40-41 0.07968095722719681 1106 42-43 0.11395767626334674 1106 44-45 5.246528626443592E-4 1106 46-47 0.2502261483033408 1106 48-49 0.3128437917507867 1106 50-51 0.1880960715999862 1106 52-53 0.2616307823694015 1106 54-55 0.18625176220835016 1106 56-57 0.23423104414967355 1106 58-59 0.25374457862060495 1106 60-61 0.22514575118513847 1106 62-63 0.33890602898301836 1106 64-65 0.2425213045844643 1106 66-67 0.0949821317109425 1106 68-69 -0.01577920294833035 1106 70-71 0.03259103692942489 1106 72-73 0.025508748174956963 1106 74-75 -0.05744937486802826 1106 76-77 -0.007253199037251079 1106 78-79 -0.05190022567713726 1106 80-81 0.038023581485759905 1106 82-83 0.18868880849977643 1106 84-85 0.21269185598019646 1106 86-87 0.10927491535117895 1106 88-89 0.09637158782837218 1106 90-91 0.057047293245112485 1106 92-93 0.18112777063404195 1106 94-95 0.17866419220035112 1106 96-97 0.2513083010919104 1106 98-99 0.0925017163406423 1106 100-101 0.08829001157007355 1107 1 0.26638753599098663 1107 2 0.20698705708277032 1107 3 0.18219726553406446 1107 4 0.16467914572205444 1107 5 0.12872049121961027 1107 6 0.1575241962698115 1107 7 0.06695130126317395 1107 8 0.07678594170236153 1107 9 0.11549152500052884 1107 10-11 0.14737702026896926 1107 12-13 0.1480560542102154 1107 14-15 0.20217128759660596 1107 16-17 0.11253436241306503 1107 18-19 0.08987020701976434 1107 20-21 0.10011590570289286 1107 22-23 0.1644667630111698 1107 24-25 0.11945059925975698 1107 26-27 0.15985123444057336 1107 28-29 0.08040907830233834 1107 30-31 0.08512035090003423 1107 32-33 0.5188484159040883 1107 34-35 0.40699959825192167 1107 36-37 0.1160152309309126 1107 38-39 0.09078629146646477 1107 40-41 0.144234696171182 1107 42-43 0.19446856104252674 1107 44-45 0.21422517997780943 1107 46-47 0.11748470275701806 1107 48-49 0.06862865729174672 1107 50-51 0.19908359356312388 1107 52-53 0.2081345496048712 1107 54-55 0.17000797889139818 1107 56-57 0.14033605025468177 1107 58-59 0.08439096475478891 1107 60-61 0.11467938827975388 1107 62-63 0.21235520852975753 1107 64-65 0.2521188208892937 1107 66-67 0.3669214390161031 1107 68-69 0.3076975235771755 1107 70-71 0.3730493590950701 1107 72-73 0.2991686248836203 1107 74-75 0.31353025525793754 1107 76-77 0.11584010332434502 1107 78-79 0.26554669055026636 1107 80-81 0.15280737492809493 1107 82-83 0.2191825694813474 1107 84-85 0.2742697767044646 1107 86-87 0.2687071497834168 1107 88-89 0.2792410896003119 1107 90-91 0.2919619720622322 1107 92-93 0.3342435572864133 1107 94-95 0.23878063402410987 1107 96-97 0.26818566492049456 1107 98-99 0.2311248141100819 1107 100-101 0.32612615916231746 1108 1 0.14685435599268715 1108 2 0.08990297837078742 1108 3 0.08624502655857214 1108 4 0.08049821037833027 1108 5 0.03131724846753059 1108 6 0.13189736831740362 1108 7 -0.008794787273608051 1108 8 0.027710211231287474 1108 9 -0.016921798110473674 1108 10-11 0.06432693082352614 1108 12-13 0.05561632339838951 1108 14-15 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-0.5222742902245088 2316 58-59 -0.41975877136215445 2316 60-61 -0.6121362672244288 2316 62-63 -1.0192290238741464 2316 64-65 -0.5153768589014689 2316 66-67 -0.47938653352137806 2316 68-69 -0.4584320359950027 2316 70-71 -0.45369857421024307 2316 72-73 -0.48124331290537015 2316 74-75 -0.3655015508230477 2316 76-77 -0.3796365208408048 2316 78-79 -0.37972676701827623 2316 80-81 -0.5008426180661658 2316 82-83 -0.7436075851120876 2316 84-85 -0.6827920139638906 2316 86-87 -0.5187175384609475 2316 88-89 -0.4184983901063859 2316 90-91 -0.48808333175356466 2316 92-93 -0.26197567581807135 2316 94-95 -0.2878925713376148 2316 96-97 -0.2628217611688939 2316 98-99 -0.2841383683662144 2316 100-101 -0.2770194202127705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 1.0 12 2.0 13 19.0 14 65.0 15 231.0 16 799.0 17 1787.0 18 3215.0 19 4807.0 20 7016.0 21 10503.0 22 12732.0 23 11245.0 24 8847.0 25 7461.0 26 7298.0 27 8438.0 28 10146.0 29 12384.0 30 12435.0 31 13798.0 32 17053.0 33 21906.0 34 29494.0 35 43164.0 36 74811.0 37 192754.0 38 472851.0 39 229021.0 40 607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.732119261797465 22.417350347809816 15.44318446902848 21.407345921364236 2 13.449272403861745 23.727478432221492 43.11102806753857 19.712221096378194 3 15.552506356537336 30.848199550412343 33.64729839960951 19.95199569344081 4 10.809776350306324 20.40331190205541 43.16132064522661 25.625591102411654 5 10.026907763741768 40.906138904642475 37.651525939364106 11.415427392251651 6 23.61339412342342 42.90730608754201 20.907143109958014 12.57215667907656 7 22.187175639625284 35.97845403414792 25.95492105876165 15.879449267465148 8 22.20989372709157 37.49365169385566 23.912021736929486 16.384432842123285 9 25.28514903806185 16.75450719447259 23.039186231522006 34.92115753594355 10-11 21.087035791688308 28.743854386936768 32.68490753491465 17.484202286460267 12-13 22.67425637361706 27.305371428383285 31.421378543424883 18.598993654574773 14-15 19.967099929129446 28.64232264458293 29.78143718243036 21.609140243857265 16-17 18.62175289799661 31.404175354002952 30.44927487321908 19.52479687478136 18-19 18.489107253243294 29.832882126873933 32.97983111242079 18.698179507461987 20-21 19.65715442784579 29.26933362746123 32.72519921540286 18.348312729290118 22-23 19.273951325674485 29.518779874079236 32.44677917607423 18.760489624172045 24-25 18.933715041102452 29.706121783764967 32.698447844292204 18.66171533084038 26-27 18.67924776790675 30.093728573180638 32.72227714255847 18.504746516354142 28-29 18.790862719371532 29.209863271684455 33.37525753339188 18.62401647555213 30-31 19.50047370504844 29.519973396790327 32.503327459006606 18.47622543915462 32-33 18.810617578037867 29.652289793899207 32.64580937713754 18.89128325092539 34-35 18.95207059727992 29.726782073453506 33.058932858997224 18.26221447026935 36-37 19.345768468117715 29.272502636038954 32.40562322051937 18.97610567532396 38-39 18.29727934440209 29.91828073465027 32.764379685091086 19.02006023585655 40-41 18.843665810348416 29.267028893950158 33.18433505557289 18.70497024012854 42-43 19.092888694815453 29.20463136770875 32.36998097357826 19.33249896389754 44-45 18.754233918927707 30.02886678722618 32.143830187234904 19.07306910661121 46-47 19.24692805120573 29.233642613494187 31.758575445481963 19.76085388981812 48-49 19.011522958253455 29.39774249122752 31.66484069606932 19.925893854449704 50-51 18.572321657501902 29.721904520455993 31.492807776186872 20.21296604585524 52-53 18.942604727502303 29.745466877275412 31.77087491799676 19.541053477225528 54-55 19.083646187188812 29.40107384119234 31.198189796450876 20.31709017516798 56-57 19.04952790003383 30.008741524959852 30.644847974015775 20.296882600990543 58-59 18.73225663866141 29.769172707675008 31.349602556937207 20.14896809672637 60-61 19.418136621422516 30.17804081027912 30.318629670175902 20.085192898122465 62-63 18.484292006443376 30.507675174151426 30.60813686166084 20.399895957744356 64-65 18.57602863137516 30.19768851691222 30.644066010860232 20.58221684085239 66-67 18.907251761680676 30.286050353488502 29.9820724657603 20.824625419070518 68-69 18.682186144005836 30.353978913628353 30.120659944084117 20.843174998281697 70-71 18.872022263725714 29.57425810216719 30.645177221660212 20.908542412446877 72-73 18.918240401813826 29.503840262212822 30.21283390855641 21.365085427416947 74-75 18.561261282395407 30.03484676190982 29.736548273280594 21.667343682414174 76-77 18.73048693257255 29.693239969676295 30.362024247442776 21.21424885030838 78-79 18.917046879102735 29.661344104121273 29.746125372564286 21.675483644211702 80-81 18.61846042155222 29.68702542038751 29.694186556654056 22.000327601406216 82-83 19.043724910300593 29.427742900391884 29.699289895142854 21.82924229416466 84-85 19.10508844003289 29.086930432442088 29.45774559199138 22.350235535533642 86-87 18.371472620132373 29.47783706988869 29.84210903218319 22.30858127779575 88-89 19.183165820976544 28.813209146047097 29.777250613329404 22.226374419646955 90-91 19.085143510935147 28.750463004881098 29.677501666817573 22.486891817366182 92-93 18.91754075056939 29.018611546221017 29.451448730791483 22.612398972418102 94-95 19.057635623278138 28.257761395878312 29.80732427847437 22.877278702369182 96-97 19.14319885487669 28.167094825790954 29.632164531632878 23.057541787699474 98-99 18.706733768938367 28.540031707305026 29.52623425541337 23.227000268343236 100-101 18.992085670660185 28.319470567173028 29.795741983809165 22.892701778357623 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 207.0 1 137.0 2 79.5 3 99.0 4 96.5 5 99.0 6 176.0 7 325.0 8 572.5 9 786.0 10 780.0 11 766.0 12 880.5 13 996.5 14 1127.5 15 1318.5 16 1464.0 17 1622.0 18 1795.5 19 2035.0 20 2345.5 21 2755.0 22 3311.0 23 3957.0 24 4939.5 25 6441.5 26 9011.5 27 12620.5 28 16746.5 29 22716.0 30 29222.0 31 34816.5 32 41262.0 33 51547.5 34 62685.0 35 63420.0 36 62016.5 37 62933.5 38 58583.5 39 54573.0 40 51567.5 41 49138.0 42 47647.5 43 45357.5 44 43419.5 45 41585.0 46 38692.5 47 35487.0 48 33307.5 49 31718.5 50 29198.5 51 26320.5 52 23475.5 53 20062.5 54 16957.0 55 14297.5 56 11426.5 57 8795.0 58 7019.5 59 5688.5 60 4309.0 61 2839.0 62 1716.5 63 1136.5 64 797.0 65 580.0 66 397.0 67 284.0 68 188.0 69 94.5 70 63.5 71 55.5 72 36.5 73 23.5 74 17.5 75 12.5 76 7.0 77 3.0 78 1.0 79 1.0 80 1.0 81 0.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1422349823975978 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 1.2346786666458142E-4 44-45 0.0 46-47 0.0022224215999624656 48-49 9.877429333166514E-4 50-51 2.0577977777430238E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 8.231191110972095E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.002510513288846489 70-71 0.0 72-73 0.0 74-75 4.115595555486047E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.646238222194419E-4 88-89 4.527155111034653E-4 90-91 8.231191110972095E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0023870454221819075 100-101 4.938714666583257E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1214891.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.30088329902265 #Duplication Level Percentage of deduplicated Percentage of total 1 64.04118996479804 24.5283414317227 2 15.500773031512677 11.873865978492093 3 7.061761718590831 8.114151344077593 4 3.9035627027757416 5.980395981177247 5 2.4000523291892004 4.596206208591153 6 1.5930322581378826 3.660872556631055 7 1.0897012876448924 2.921556529421722 8 0.8015333891235732 2.4559549437673676 9 0.5834208635348292 2.0110980967616268 >10 2.767596526129208 18.142996471972577 >50 0.14823985453170638 3.9037536802666515 >100 0.10371181677520189 7.324487164878396 >500 0.002820613773255081 0.664479895148174 >1k 0.001518792031752736 1.0756663035014964 >5k 0.0010848514512519542 2.746173413590115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 8193 0.6743814877219437 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6766 0.556922390568372 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 6486 0.5338750554576501 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 6234 0.5131324538580004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5367 0.4417680269258724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.2577185936845363 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 2521 0.20750832790760654 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1948 0.1603436028417364 TruSeq Adapter, Index 1 (95% over 21bp) TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 1786 0.14700907324196164 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 1284 0.1056884938648817 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 1238 0.10190214595383455 RNA PCR Primer, Index 1 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.231191110972096E-5 0.0 3 0.0 0.0 0.0 8.231191110972096E-5 0.0 4 0.0 0.0 0.0 8.231191110972096E-5 0.0 5 0.0 0.0 0.0 8.231191110972096E-5 0.0 6 0.0 0.0 0.0 8.231191110972096E-5 0.0 7 0.0 0.0 0.0 8.231191110972096E-5 0.0 8 0.0 0.0 0.0 8.231191110972096E-5 0.0 9 0.0 0.0 0.0 8.231191110972096E-5 0.0 10-11 0.0 0.0 0.0 1.6462382221944192E-4 0.0 12-13 0.0 0.0 0.0 2.4693573332916285E-4 0.0 14-15 0.0 0.0 0.0 4.1155955554860477E-4 0.0 16-17 0.0 0.0 0.0 5.761833777680467E-4 0.0 18-19 0.0 0.0 0.0 6.996512444326281E-4 0.0 20-21 0.0 0.0 0.0 8.6427506665207E-4 0.0 22-23 0.0 0.0 0.0 0.002304733511072187 0.0 24-25 0.0 0.0 0.0 0.004938714666583258 0.0 26-27 0.0 0.0 0.0 0.008601594710965839 0.0 28-29 0.0 0.0 0.0 0.021071849244088565 0.0 30-31 0.0 0.0 0.0 0.055519384043506786 0.0 32-33 0.0 0.0 0.0 0.1330983602644188 0.0 34-35 0.0 0.0 0.0 0.25413802555126347 0.0 36-37 0.0 0.0 0.0 0.45818925319226167 0.0 38-39 0.0 0.0 0.0 0.7353334578986921 0.0 40-41 0.0 0.0 0.0 1.0183218082939127 0.0 42-43 0.0 0.0 0.0 1.294684049844801 0.0 44-45 0.0 0.0 0.0 1.624466721705898 0.0 46-47 0.0 0.0 0.0 2.0364378368100513 0.0 48-49 0.0 0.0 0.0 2.460344179025114 0.0 50-51 0.0 0.0 0.0 2.867952762840452 0.0 52-53 0.0 0.0 0.0 3.256958854744994 0.0 54-55 0.0 0.0 0.0 3.6734159690046266 0.0 56-57 0.0 0.0 0.0 4.1665466284629655 0.0 58-59 0.0 0.0 0.0 4.7073358844538316 0.0 60-61 0.0 0.0 0.0 5.2429806460003405 0.0 62-63 0.0 0.0 0.0 5.74796422065848 0.0 64-65 0.0 0.0 0.0 6.283691294116098 0.0 66-67 0.0 0.0 0.0 6.8625909649507655 0.0 68-69 0.0 0.0 0.0 7.519645795384113 0.0 70-71 0.0 0.0 0.0 8.209460766439129 0.0 72-73 0.0 0.0 0.0 8.862976184694759 0.0 74-75 0.0 0.0 0.0 9.528879545572401 0.0 76-77 0.0 0.0 0.0 10.221205029916264 0.0 78-79 0.0 0.0 0.0 10.978104208525703 0.0 80-81 0.0 0.0 0.0 11.790769706911977 0.0 82-83 0.0 0.0 0.0 12.602365150453826 0.0 84-85 0.0 0.0 0.0 13.38766193839612 0.0 86-87 0.0 0.0 0.0 14.21349734255995 0.0 88-89 0.0 0.0 0.0 15.108762843744831 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2950 0.0 57.24683 1 GTATCAA 5515 0.0 42.093925 1 TCAACGC 6685 0.0 34.46066 4 ATCAACG 6720 0.0 34.281178 3 AACGCAG 6745 0.0 34.154118 6 CAACGCA 6740 0.0 34.038506 5 TATCAAC 6860 0.0 33.581562 2 GTACATG 5880 0.0 30.66246 1 ACGCAGA 7500 0.0 30.589272 7 ACATGGG 5645 0.0 30.544003 3 CGCAGAG 7525 0.0 30.487644 8 CATGGGA 3565 0.0 30.24472 4 CTTCGGC 65 0.005844882 29.230072 9 TACATGG 6205 0.0 29.012201 2 GCAGAGT 8220 0.0 27.447643 9 ACGCAGT 70 0.008392731 27.14221 8 TAGCCCG 70 0.008392731 27.14221 4 GAGTACT 5185 0.0 24.82583 12-13 CAACGCG 60 1.7118572E-4 23.749434 34-35 ACCGTGA 140 1.7799179E-5 23.749434 8 >>END_MODULE