FastQCFastQC Report
Fri 10 Feb 2017
SRR2031568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665886
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.3165707042947291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.26295792372868626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.12689859825856079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.053.8333361
GTATCAA19500.044.5769231
TCAACGC21550.038.7935034
ATCAACG22150.037.5282173
CAACGCA22350.037.4049225
TATCAAC22950.036.6339872
AACGCAG23350.036.0064246
ACGCAGA26250.032.0285727
CGCAGAG26900.031.2546468
ACGTCGT350.00839204127.14285922-23
GCAGAGT31100.027.0337629
GTACATG20700.025.4710141
GTATAGC3600.025.0694451
GTGTACG950.001226012825.01
GTGGTAT5350.024.8598161
TACATGG20900.024.3181822
TGGTATC4600.023.7499982
CAGAGTA29250.023.14102610-11
ACATGGG21650.023.0369513
GAGTACT19500.022.89743612-13