##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031568_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 665886 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.100386552653156 34.0 31.0 34.0 30.0 34.0 2 32.25917199040076 34.0 31.0 34.0 30.0 34.0 3 32.361949342680276 34.0 31.0 34.0 30.0 34.0 4 35.602190164682845 37.0 35.0 37.0 33.0 37.0 5 35.58488840432146 37.0 35.0 37.0 33.0 37.0 6 35.71643644707953 37.0 36.0 37.0 35.0 37.0 7 35.74651967453889 37.0 36.0 37.0 35.0 37.0 8 35.79212357670833 37.0 37.0 37.0 35.0 37.0 9 37.6028554437246 39.0 39.0 39.0 35.0 39.0 10-11 37.59518896627951 39.0 38.0 39.0 35.0 39.0 12-13 37.55969940800678 39.0 38.0 39.0 35.0 39.0 14-15 39.04298558612135 41.0 39.0 41.0 36.0 41.0 16-17 39.04977128217142 41.0 39.0 41.0 36.0 41.0 18-19 39.01718086879736 41.0 39.0 41.0 36.0 41.0 20-21 38.97535764380089 41.0 39.0 41.0 36.0 41.0 22-23 38.90357808994332 41.0 39.0 41.0 35.0 41.0 24-25 38.8486114740361 41.0 39.0 41.0 35.0 41.0 26-27 38.72554836713792 41.0 39.0 41.0 35.0 41.0 28-29 38.61305989313486 40.5 39.0 41.0 34.0 41.0 30-31 38.43776787618301 40.0 38.0 41.0 34.0 41.0 32-33 38.31583709523852 40.0 38.0 41.0 34.0 41.0 34-35 38.16814664972683 40.0 38.0 41.0 33.5 41.0 36-37 38.01724319177757 40.0 38.0 41.0 33.0 41.0 38-39 37.86302835620512 40.0 38.0 41.0 33.0 41.0 40-41 37.71215192990992 40.0 38.0 41.0 32.5 41.0 42-43 37.54680605989614 40.0 37.5 41.0 32.0 41.0 44-45 37.70978140402411 40.0 38.0 41.0 32.5 41.0 46-47 37.5591933153723 40.0 38.0 41.0 31.5 41.0 48-49 37.392403504503775 40.0 37.5 41.0 31.0 41.0 50-51 36.337005133010756 39.0 36.0 40.0 29.5 40.5 52-53 36.410110739676156 39.0 36.5 40.0 30.0 40.5 54-55 37.18776787618301 40.0 37.0 41.0 31.0 41.0 56-57 37.224388258650876 40.0 37.0 41.0 31.0 41.0 58-59 37.04646065542751 40.0 36.5 41.0 31.0 41.0 60-61 36.87588190771393 40.0 36.0 41.0 30.0 41.0 62-63 36.61672643665733 39.0 35.5 41.0 30.0 41.0 64-65 36.34381861159417 39.0 35.0 41.0 29.0 41.0 66-67 36.05589019742118 39.0 35.0 41.0 29.0 41.0 68-69 35.761046935962014 37.5 35.0 40.5 29.0 41.0 70-71 35.39684420456355 37.0 35.0 40.0 28.5 41.0 72-73 35.03633655010017 37.0 35.0 39.0 28.0 41.0 74-75 34.658235043235635 36.0 34.5 39.0 28.0 41.0 76-77 34.2786580585866 36.0 34.0 38.5 27.5 40.5 78-79 33.882862081497436 35.0 34.0 37.0 26.5 39.0 80-81 33.5399452759181 35.0 34.0 37.0 26.0 39.0 82-83 33.23595029779872 35.0 34.0 36.5 26.0 39.0 84-85 32.93615348573179 35.0 34.0 36.0 26.0 37.0 86-87 32.67296429118498 35.0 34.0 36.0 25.0 37.0 88-89 32.460502999011844 35.0 33.5 35.0 25.0 37.0 90-91 32.27651880351892 35.0 33.0 35.0 25.0 36.0 92-93 32.1171108868485 35.0 33.0 35.0 24.5 36.0 94-95 31.98653298011972 35.0 33.0 35.0 24.0 36.0 96-97 31.852229210405383 35.0 33.0 35.0 24.0 36.0 98-99 31.72954830106054 35.0 33.0 35.0 23.0 35.0 100-101 30.22591855062278 33.5 29.5 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29671171503134275 1101 2 0.3028235461024238 1101 3 0.3872812675605033 1101 4 0.11744308936170711 1101 5 0.21498644554041135 1101 6 0.18212185151497806 1101 7 0.20760910085584783 1101 8 0.3521269447745681 1101 9 0.6070529620857528 1101 10-11 0.52525260648369 1101 12-13 0.6158219081922454 1101 14-15 0.8229676089996545 1101 16-17 0.7531495547562059 1101 18-19 0.7357907795126906 1101 20-21 0.795220960022867 1101 22-23 0.7173003569012266 1101 24-25 0.6800013309476611 1101 26-27 0.7412694574517573 1101 28-29 0.7777480479991894 1101 30-31 0.7543168489889993 1101 32-33 0.7891874917180601 1101 34-35 0.6854487630063062 1101 36-37 0.8307147463353104 1101 38-39 0.9140913620705007 1101 40-41 0.6994098165181271 1101 42-43 0.7671626574513724 1101 44-45 0.9326789215775833 1101 46-47 1.1152447335592939 1101 48-49 0.989301146853542 1101 50-51 1.2243210616065099 1101 52-53 1.0790602263071705 1101 54-55 0.8574704991235933 1101 56-57 0.8271643427713613 1101 58-59 0.766608426987041 1101 60-61 0.8272658039935052 1101 62-63 0.977051595402969 1101 64-65 0.9807886354660056 1101 66-67 1.1267447061911398 1101 68-69 1.0007976020144795 1101 70-71 0.9221288415635271 1101 72-73 0.9699798769121912 1101 74-75 0.8579959553141663 1101 76-77 0.9367838738101852 1101 78-79 0.8865375345365507 1101 80-81 0.9357809121203857 1101 82-83 0.8584412779304955 1101 84-85 0.5786918920391315 1101 86-87 0.6040931169037336 1101 88-89 0.4449693425104613 1101 90-91 0.3190055314467486 1101 92-93 0.4400225726960727 1101 94-95 0.6920584074719898 1101 96-97 0.7444081939818865 1101 98-99 0.7343224708830363 1101 100-101 0.8460437949763389 1102 1 -0.173554022842751 1102 2 0.02173479481243845 1102 3 0.05665691255534 1102 4 -0.32237889206244574 1102 5 -0.08709688779292435 1102 6 -0.005410398227027713 1102 7 0.033202496108685864 1102 8 0.025533807519671825 1102 9 0.05848485063064146 1102 10-11 0.2545482721183632 1102 12-13 0.16256790922423647 1102 14-15 0.29052436854558294 1102 16-17 0.29310698509675603 1102 18-19 0.24335334401217779 1102 20-21 0.26371296208684925 1102 22-23 0.3762754601004019 1102 24-25 0.21360556211381265 1102 26-27 0.3884572799491792 1102 28-29 0.4199532853572947 1102 30-31 0.4606506983182044 1102 32-33 0.45616053093374376 1102 34-35 0.29487213761930775 1102 36-37 0.28035677419908467 1102 38-39 0.3479632660952703 1102 40-41 0.5257449558370126 1102 42-43 0.4092388958414688 1102 44-45 0.5872196852514691 1102 46-47 0.6006839750453707 1102 48-49 0.34075883519202677 1102 50-51 0.3122661080461384 1102 52-53 0.4911474296095477 1102 54-55 0.47640754762720405 1102 56-57 0.5600397297682633 1102 58-59 0.42926000077445536 1102 60-61 0.54329070079433 1102 62-63 0.5549508472089499 1102 64-65 0.739238067870545 1102 66-67 0.37095587750177117 1102 68-69 0.535546699021701 1102 70-71 0.464198630005221 1102 72-73 0.3797612236614256 1102 74-75 0.2031971937042627 1102 76-77 0.20745595843350628 1102 78-79 0.17065776157473067 1102 80-81 0.14009915257445726 1102 82-83 0.14767308907600096 1102 84-85 0.3996090747016652 1102 86-87 0.3148935555002268 1102 88-89 0.26011382135462924 1102 90-91 0.24921708975428203 1102 92-93 0.03340170066305603 1102 94-95 0.10719321139355031 1102 96-97 0.08188561916248815 1102 98-99 0.23501584033608225 1102 100-101 0.1733948011682891 1103 1 0.3040644341786134 1103 2 0.23098355455729802 1103 3 0.42130457885077277 1103 4 0.30456375246524914 1103 5 0.1871608574738559 1103 6 0.13650706152795777 1103 7 0.2627129749990331 1103 8 0.2629129459220181 1103 9 0.3619107390583025 1103 10-11 0.5037889322378319 1103 12-13 0.43852625943178225 1103 14-15 0.5393727030601099 1103 16-17 0.48996901304046503 1103 18-19 0.5342232311236472 1103 20-21 0.6207071151697718 1103 22-23 0.621341332835037 1103 24-25 0.6034696402751933 1103 26-27 0.5351744005323553 1103 28-29 0.4945853219068468 1103 30-31 0.6742276953820863 1103 32-33 0.6207996546555208 1103 34-35 0.5188236158009474 1103 36-37 0.6698079613004282 1103 38-39 0.5827717226104099 1103 40-41 0.706085013546847 1103 42-43 0.8730769159892873 1103 44-45 0.8067258612158383 1103 46-47 0.5267290165559473 1103 48-49 0.7312746800842049 1103 50-51 0.7815160068008211 1103 52-53 0.8512580401188075 1103 54-55 0.9172170039398466 1103 56-57 0.8114982951221137 1103 58-59 1.008966958858899 1103 60-61 0.891060456286894 1103 62-63 0.8201835216040081 1103 64-65 0.7695357291037155 1103 66-67 0.8309940343307645 1103 68-69 0.822312986862137 1103 70-71 0.8578831369405293 1103 72-73 0.8208665061358573 1103 74-75 0.824602984936412 1103 76-77 0.6570389542824486 1103 78-79 0.5009010034497905 1103 80-81 0.8550255598843748 1103 82-83 0.7133987016101813 1103 84-85 0.580430047669168 1103 86-87 0.7028830133315509 1103 88-89 0.5917934811703631 1103 90-91 0.6348478480817121 1103 92-93 0.6359774448161417 1103 94-95 0.6307058544025637 1103 96-97 0.7537859304918335 1103 98-99 0.6017659289257367 1103 100-101 0.5856261846855517 1104 1 0.10936207001579845 1104 2 0.10254337242407985 1104 3 0.03656543114672672 1104 4 -0.017502342780524316 1104 5 -0.06817966371265527 1104 6 -0.07096592645838484 1104 7 0.01434060352556088 1104 8 0.14699873964636367 1104 9 0.18302990276520603 1104 10-11 0.1885931275187147 1104 12-13 0.27487547857303696 1104 14-15 0.4718887203063531 1104 16-17 0.32598004234641564 1104 18-19 0.3954934433629518 1104 20-21 0.3645822813864825 1104 22-23 0.42407879966364703 1104 24-25 0.45515828688265003 1104 26-27 0.4409115392448655 1104 28-29 0.5030458242123004 1104 30-31 0.41899222891037624 1104 32-33 0.4419188183624243 1104 34-35 0.3813429717723693 1104 36-37 0.2856962636738061 1104 38-39 0.3372012899001149 1104 40-41 0.3687572025417083 1104 42-43 0.4481277164198616 1104 44-45 0.31438622076199607 1104 46-47 0.46212468427219733 1104 48-49 0.5815861025538211 1104 50-51 0.43816985583288215 1104 52-53 0.2357741264421236 1104 54-55 0.2932725879577234 1104 56-57 0.3018937037993439 1104 58-59 0.6245156322912777 1104 60-61 0.32680007026118574 1104 62-63 0.5035140726663556 1104 64-65 0.47728646448671697 1104 66-67 0.2867444128155654 1104 68-69 0.30676632817771576 1104 70-71 0.35168203055607705 1104 72-73 0.2512353777043117 1104 74-75 0.13992255274420273 1104 76-77 0.4267100898519587 1104 78-79 0.19981131262725427 1104 80-81 0.3217678275695377 1104 82-83 0.10907936981572419 1104 84-85 0.10805512034400522 1104 86-87 -0.07280502309509274 1104 88-89 0.14340125003847248 1104 90-91 0.2850473081292648 1104 92-93 0.04748971463119034 1104 94-95 0.01974792809628667 1104 96-97 0.14801598016976314 1104 98-99 0.0822732424608148 1104 100-101 0.2476896319768649 1105 1 0.38445903861185826 1105 2 0.18415727179044694 1105 3 0.3937411072555861 1105 4 0.4111415928088178 1105 5 0.23788314415595835 1105 6 0.1494653300195452 1105 7 0.17262647656348662 1105 8 0.1742781694817026 1105 9 0.48184277207398196 1105 10-11 0.4788951993876722 1105 12-13 0.551489751922972 1105 14-15 0.4966651315600572 1105 16-17 0.47376499111067005 1105 18-19 0.4907897986259684 1105 20-21 0.5167962640977564 1105 22-23 0.5241007044153747 1105 24-25 0.4844545566637066 1105 26-27 0.5595952239446689 1105 28-29 0.5859846939271094 1105 30-31 0.6426183735672168 1105 32-33 0.3661310486935676 1105 34-35 0.4554798711280412 1105 36-37 0.584960584573075 1105 38-39 0.6994978695532694 1105 40-41 0.7727556211254907 1105 42-43 0.5432157094103474 1105 44-45 0.6178177871120241 1105 46-47 0.4828460451449672 1105 48-49 0.30000051908603353 1105 50-51 0.4112430390741366 1105 52-53 0.3413283166608565 1105 54-55 0.4580604324232951 1105 56-57 0.23675110920822107 1105 58-59 0.23894391827116834 1105 60-61 0.35053734146337945 1105 62-63 0.5052450823151275 1105 64-65 0.5373963088026983 1105 66-67 0.45439590287294607 1105 68-69 0.4707474366650075 1105 70-71 0.5802688000860314 1105 72-73 0.6940319199591087 1105 74-75 0.5919164194137139 1105 76-77 0.42891926420310256 1105 78-79 0.6180478198344517 1105 80-81 0.4875226866845921 1105 82-83 0.33050702536618104 1105 84-85 0.4540807435609011 1105 86-87 0.5412813350529433 1105 88-89 0.473043721749292 1105 90-91 0.48735371820758644 1105 92-93 0.7853192208660218 1105 94-95 0.8087383861727346 1105 96-97 0.49455182382326157 1105 98-99 0.5951836894246014 1105 100-101 0.5974310490540269 1106 1 0.04038873091490558 1106 2 -0.037877378132449735 1106 3 -0.06588437525315527 1106 4 0.0811862406152315 1106 5 0.0031042163080243768 1106 6 -0.04800294786517867 1106 7 0.0881987848449981 1106 8 0.04034986801326568 1106 9 0.22483064762451477 1106 10-11 0.09723708265835285 1106 12-13 0.16298832581357914 1106 14-15 0.09324750827465778 1106 16-17 0.05798103408544364 1106 18-19 0.10581333195057141 1106 20-21 0.21092603501318052 1106 22-23 0.14174969001318516 1106 24-25 0.12461641756009101 1106 26-27 0.3305383710606975 1106 28-29 0.2719231545352443 1106 30-31 0.31577224220389866 1106 32-33 0.18718959476748154 1106 34-35 0.166874132423537 1106 36-37 0.28796196532916696 1106 38-39 0.3176381825918426 1106 40-41 0.46139512284387507 1106 42-43 0.40964771473180406 1106 44-45 0.2713563525366851 1106 46-47 0.35526216673213185 1106 48-49 0.4041518580162986 1106 50-51 0.45367105330500124 1106 52-53 0.37833661127590545 1106 54-55 0.28840884656949584 1106 56-57 0.3968659034554065 1106 58-59 0.3939363089949026 1106 60-61 0.23897577687523608 1106 62-63 0.29796033412674205 1106 64-65 0.2354340225931324 1106 66-67 0.18704826477213743 1106 68-69 0.008967672300606466 1106 70-71 0.14378609985119795 1106 72-73 0.14538042481191127 1106 74-75 0.02670341007092958 1106 76-77 0.10786071812918863 1106 78-79 0.2264774869412136 1106 80-81 0.2816287730977436 1106 82-83 0.18400578066999174 1106 84-85 0.3429601138555043 1106 86-87 0.260625022377198 1106 88-89 0.3763227655001131 1106 90-91 0.4574885686928667 1106 92-93 0.4043917962280972 1106 94-95 0.14585704048985804 1106 96-97 0.3530369227438577 1106 98-99 0.3419031338970413 1106 100-101 0.4034902502311972 1107 1 0.09061087735155837 1107 2 -0.025827473338068785 1107 3 0.03636456138063693 1107 4 0.08387662816366515 1107 5 0.10087233142629515 1107 6 0.08644064399166496 1107 7 -0.09409391795243494 1107 8 0.035310127957750126 1107 9 0.3230913689136301 1107 10-11 0.17806001915951697 1107 12-13 0.1467607417626482 1107 14-15 0.07724491261906508 1107 16-17 0.1674059163809858 1107 18-19 0.15388979958540006 1107 20-21 0.2869034319684971 1107 22-23 0.35960713736589867 1107 24-25 0.25819794861796197 1107 26-27 0.25312341667414273 1107 28-29 0.2825686580829583 1107 30-31 0.23180420476582952 1107 32-33 0.06846677099733967 1107 34-35 0.3392933971140977 1107 36-37 0.32822541489860413 1107 38-39 0.24680236254466337 1107 40-41 0.2742379341094008 1107 42-43 0.12443111011456409 1107 44-45 0.03931516308224303 1107 46-47 0.31176362086765863 1107 48-49 0.2619520872676375 1107 50-51 0.27610310680960737 1107 52-53 0.26898040964840675 1107 54-55 0.15431339465069982 1107 56-57 0.3794896944388242 1107 58-59 0.36781160384811784 1107 60-61 0.3000115760813813 1107 62-63 0.2701209805776159 1107 64-65 0.4312094510183968 1107 66-67 0.528732220020764 1107 68-69 0.503267176852475 1107 70-71 0.4666799716409713 1107 72-73 0.5232733019161486 1107 74-75 0.31304969326264853 1107 76-77 0.25436131967710907 1107 78-79 0.15441725636363657 1107 80-81 0.4279375424349112 1107 82-83 0.40046356337383315 1107 84-85 0.4814795481162619 1107 86-87 0.4907099969416677 1107 88-89 0.4578704014641488 1107 90-91 0.4420496281750559 1107 92-93 0.4301541516434426 1107 94-95 0.4148436927572732 1107 96-97 0.45460852589274126 1107 98-99 0.34383329749913116 1107 100-101 0.4693644840865012 1108 1 0.21275181528196896 1108 2 0.2256932202878872 1108 3 0.03703064099408948 1108 4 0.008232563045915242 1108 5 0.12150273626722452 1108 6 0.14032987156510046 1108 7 0.0783484492267732 1108 8 0.17042268412293993 1108 9 0.289947698927854 1108 10-11 0.16801576437842414 1108 12-13 0.2982780485431107 1108 14-15 0.19555532829790678 1108 16-17 0.07908940438026946 1108 18-19 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-0.14152078434272397 2316 92-93 -0.17832538052029534 2316 94-95 -0.3874006568554975 2316 96-97 -0.12613274169062905 2316 98-99 -0.09915706128070312 2316 100-101 0.06738882421610626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 7.0 7 99.0 8 183.0 9 196.0 10 247.0 11 460.0 12 808.0 13 1025.0 14 1206.0 15 1344.0 16 1546.0 17 1805.0 18 2244.0 19 2858.0 20 3611.0 21 4674.0 22 5480.0 23 5536.0 24 5327.0 25 4888.0 26 4807.0 27 5103.0 28 5696.0 29 6558.0 30 8066.0 31 10002.0 32 12393.0 33 16633.0 34 23717.0 35 36898.0 36 67044.0 37 140185.0 38 211151.0 39 73992.0 40 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.000474555704734 19.015266877513568 13.794853773769084 23.18940479301262 2 14.807039042719024 20.227786738270513 41.79364035285319 23.171533866157272 3 18.23960858164911 26.282877249258885 30.427130169428402 25.050383999663605 4 11.69359319763443 17.597306445848087 40.28166983537723 30.427430521140252 5 12.60831433608756 38.51199754913004 35.74590845880526 13.133779655977149 6 29.503548655475566 38.68139591461602 18.662653967796288 13.152401462112135 7 26.077586854206274 33.57301399939329 22.67775565186834 17.6716434945321 8 24.8731764896694 36.51766218241561 20.360692370766166 18.248468957148823 9 25.777535494063546 16.642938881430155 20.50636295101564 37.07316267349066 10-11 23.931198433365473 26.860303415299313 29.306067404931174 19.90243074640404 12-13 25.243810502097958 24.37609440655007 28.96817172909477 21.411923362257205 14-15 22.127286051966852 25.840008650129302 26.870290109718482 25.162415188185367 16-17 21.491291302114774 29.060079352922273 27.4190326872768 22.029596657686152 18-19 21.359737252322468 28.09730194057241 29.429511958503408 21.113448848601713 20-21 22.26026677239047 27.16959059058157 29.656427676809543 20.913714960218417 22-23 21.880997648246094 27.141507705523164 29.281889092126878 21.695605554103857 24-25 21.602496523428936 27.349726529766354 29.471711374018977 21.576065572785733 26-27 21.64049101497854 27.486536734516122 29.335952400260705 21.537019850244636 28-29 21.6009196769417 27.55231375941227 29.34849208423063 21.498274479415393 30-31 21.937013242506975 27.38636943861262 29.325515178273758 21.351102140606653 32-33 21.57313714359515 27.36609569806243 29.46457802086243 21.59618913747999 34-35 21.79742478442256 27.46416053198295 29.34946822729416 21.388946456300328 36-37 21.862075490399256 27.48195637091034 29.127508312233623 21.528459826456782 38-39 21.77872789035961 27.78095650006157 29.049642130935325 21.39067347864349 40-41 21.92597531709632 27.51582102642194 29.135542720525738 21.422660935956003 42-43 21.676833572112944 27.466413169821863 29.06060496841802 21.796148289647178 44-45 21.851713356340273 27.710974551199453 28.873110412292796 21.56420168016748 46-47 21.90098748208213 27.62757260820366 28.57030225166339 21.90113765805082 48-49 21.84521351284381 27.784977886572005 28.54974958138417 21.82005901920001 50-51 21.8983429596057 28.010350119990505 28.26850241632952 21.82280450407427 52-53 21.880171681038497 28.019510847202074 28.30844919400618 21.79186827775325 54-55 21.794045827664192 27.94795205185272 28.365966546826332 21.892035573656752 56-57 21.860723907695913 28.056228843976292 28.28719931039247 21.795847937935324 58-59 21.96186734666294 27.89591611777391 28.398179267922735 21.744037267640408 60-61 21.95691154341734 27.89749296426115 28.445334486683905 21.700261005637604 62-63 21.764911711614303 27.868509024067183 28.525978921316863 21.840600343001654 64-65 21.775198757745322 27.79312074439168 28.43940254037478 21.99227795748822 66-67 21.759205029089063 27.946600469149374 28.255437116863845 22.038757384897714 68-69 21.834142781196782 27.917241089315585 28.261969766596685 21.986646362890948 70-71 21.850061421925073 27.692878360560215 28.244774631093012 22.2122855864217 72-73 21.806660599562086 27.854242317754093 28.186431311065256 22.152665771618565 74-75 21.815671150917726 27.79702531664579 28.08588857552194 22.30141495691455 76-77 21.738856200610915 27.82112854152212 28.218944383873517 22.221070873993444 78-79 21.964345248285746 27.681464995509742 28.059908152446518 22.294281603758 80-81 21.871761833106568 27.80032918547619 27.943371688246938 22.384537293170304 82-83 21.910882643575626 27.874891497944095 27.965522626996215 22.24870323148407 84-85 22.05445076184212 27.84418053540696 27.828336982606633 22.27303172014429 86-87 21.887154858339112 27.794772678806883 28.131542035723832 22.18653042713017 88-89 21.967874380900035 27.63491048017228 28.051047776946803 22.34616736198088 90-91 22.08869085699354 27.69483064668727 27.94697590878919 22.26950258753 92-93 22.2155894552521 27.600294945381044 28.05885692145502 22.125258677911834 94-95 22.08313435032423 27.59443808699988 28.033627377659236 22.288800185016655 96-97 22.010223972271532 27.46573737847019 28.227204055949517 22.296834593308766 98-99 21.995882178030474 27.38043749230349 28.054351645777203 22.569328683888834 100-101 22.32409151115957 27.37480589770621 27.965222275284358 22.33588031584986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 24.5 2 41.5 3 60.0 4 52.0 5 51.5 6 90.5 7 205.0 8 376.0 9 503.0 10 485.5 11 435.0 12 443.0 13 495.5 14 557.0 15 606.5 16 632.5 17 679.5 18 820.0 19 908.0 20 1022.0 21 1269.0 22 1609.5 23 1964.0 24 2409.0 25 3087.0 26 4013.5 27 5213.0 28 6521.0 29 7976.5 30 9717.5 31 11421.0 32 12958.5 33 14532.5 34 16136.5 35 17551.0 36 18926.0 37 20105.5 38 20688.5 39 21209.5 40 21867.5 41 22215.5 42 22716.5 43 23841.5 44 24617.5 45 25278.0 46 25502.5 47 25158.5 48 25350.0 49 25127.5 50 24236.5 51 23153.5 52 22007.5 53 20854.5 54 19642.0 55 18190.0 56 16226.0 57 14167.0 58 12402.5 59 10491.5 60 8620.0 61 7028.0 62 5461.0 63 4114.0 64 3044.5 65 2252.0 66 1657.5 67 1235.0 68 931.0 69 696.0 70 551.5 71 421.5 72 283.5 73 204.0 74 153.0 75 102.5 76 71.0 77 42.0 78 32.0 79 26.0 80 15.5 81 13.0 82 11.0 83 12.0 84 10.0 85 7.0 86 5.5 87 5.0 88 4.5 89 4.0 90 3.0 91 1.5 92 1.5 93 1.5 94 1.5 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 7.508792796364543E-5 48-49 1.5017585592729085E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 665886.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.32787343696036 #Duplication Level Percentage of deduplicated Percentage of total 1 84.1517660239341 65.07277112595477 2 10.982837704946961 16.985589680536297 3 2.67579909835419 6.207415620607964 4 0.9747149257391418 3.0149052965869028 5 0.4551275751582845 1.7597023764755244 6 0.22381249787569213 1.0384166705604887 7 0.1378491143773734 0.7461705208979422 8 0.09033686448088744 0.5588446098615923 9 0.06491570504614086 0.45178140814911266 >10 0.234649478435301 2.9228127287594847 >50 0.005460711974802933 0.2904571159439388 >100 0.0021452354615196715 0.24174186670152178 >500 1.9502140520527605E-4 0.12743099005633926 >1k 3.900428104105521E-4 0.5819599889081813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2108 0.3165707042947291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1751 0.26295792372868626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.12689859825856079 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5017585592729085E-4 0.0 10-11 0.0 0.0 0.0 1.5017585592729085E-4 0.0 12-13 0.0 0.0 0.0 2.2526378389093628E-4 0.0 14-15 0.0 0.0 0.0 3.003517118545817E-4 0.0 16-17 0.0 0.0 0.0 3.003517118545817E-4 0.0 18-19 0.0 1.5017585592729085E-4 0.0 3.003517118545817E-4 0.0 20-21 0.0 3.7543963981822713E-4 0.0 3.7543963981822713E-4 0.0 22-23 0.0 4.5052756778187256E-4 0.0 0.0012764947753819722 0.0 24-25 0.0 4.5052756778187256E-4 0.0 0.0020273740550184266 0.0 26-27 0.0 4.5052756778187256E-4 0.0 0.003003517118545817 0.0 28-29 0.0 6.007034237091634E-4 0.0 0.0054814187413461165 0.0 30-31 0.0 7.508792796364543E-4 0.0 0.012915123609747014 0.0 32-33 0.0 8.259672076000997E-4 0.0 0.03056078668120369 0.0 34-35 0.0 9.010551355637451E-4 0.0 0.05466401155753388 0.0 36-37 0.0 9.010551355637451E-4 0.0 0.09813992184848458 0.0 38-39 0.0 9.010551355637451E-4 0.0 0.16932327755802046 0.0 40-41 0.0 9.010551355637451E-4 0.0 0.25146947075024856 0.0 42-43 0.0 9.010551355637451E-4 0.0 0.3254310797944393 0.0 44-45 0.0 9.010551355637451E-4 0.0 0.4138095710076499 0.0 46-47 0.0 9.010551355637451E-4 0.0 0.5149530099746804 0.0 48-49 0.0 9.761430635273906E-4 0.0 0.6296873639031306 0.0 50-51 0.0 0.001051230991491036 0.0 0.7576371931531823 1.5017585592729085E-4 52-53 0.0 0.001051230991491036 0.0 0.8821329777169065 1.5017585592729085E-4 54-55 0.0 0.001051230991491036 0.0 1.0052020916493214 1.5017585592729085E-4 56-57 0.0 0.001051230991491036 0.0 1.150572320186939 1.5017585592729085E-4 58-59 0.0 0.0012014068474183268 0.0 1.3063797707115032 1.5017585592729085E-4 60-61 0.0 0.0012014068474183268 0.0 1.4829114893540336 1.5017585592729085E-4 62-63 0.0 0.0012014068474183268 0.0 1.6622965492591826 1.5017585592729085E-4 64-65 0.0 0.0012014068474183268 0.0 1.8253124408682568 1.5017585592729085E-4 66-67 0.0 0.0012014068474183268 0.0 2.006724874828424 1.5017585592729085E-4 68-69 0.0 0.0012014068474183268 0.0 2.2168959851986676 1.5017585592729085E-4 70-71 0.0 0.0012014068474183268 0.0 2.4453885499920407 1.5017585592729085E-4 72-73 0.0 0.0012014068474183268 0.0 2.6774853353276686 1.5017585592729085E-4 74-75 0.0 0.0012014068474183268 0.0 2.8995203383161683 1.5017585592729085E-4 76-77 0.0 0.0012764947753819722 0.0 3.1331939701390326 1.5017585592729085E-4 78-79 0.0 0.0016519344152001994 0.0 3.384738528817245 1.5017585592729085E-4 80-81 0.0 0.0018021102711274902 0.0 3.662188422642915 1.5017585592729085E-4 82-83 0.0 0.0018021102711274902 0.0 3.935508480430584 3.003517118545817E-4 84-85 0.0 0.0018021102711274902 0.0 4.216262243086654 3.003517118545817E-4 86-87 0.0 0.0018021102711274902 0.0 4.501896721060362 3.003517118545817E-4 88-89 0.0 0.0018021102711274902 0.0 4.846625398341458 3.003517118545817E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1050 0.0 53.833336 1 GTATCAA 1950 0.0 44.576923 1 TCAACGC 2155 0.0 38.793503 4 ATCAACG 2215 0.0 37.528217 3 CAACGCA 2235 0.0 37.404922 5 TATCAAC 2295 0.0 36.633987 2 AACGCAG 2335 0.0 36.006424 6 ACGCAGA 2625 0.0 32.028572 7 CGCAGAG 2690 0.0 31.254646 8 ACGTCGT 35 0.008392041 27.142859 22-23 GCAGAGT 3110 0.0 27.033762 9 GTACATG 2070 0.0 25.471014 1 GTATAGC 360 0.0 25.069445 1 GTGTACG 95 0.0012260128 25.0 1 GTGGTAT 535 0.0 24.859816 1 TACATGG 2090 0.0 24.318182 2 TGGTATC 460 0.0 23.749998 2 CAGAGTA 2925 0.0 23.141026 10-11 ACATGGG 2165 0.0 23.036951 3 GAGTACT 1950 0.0 22.897436 12-13 >>END_MODULE