FastQCFastQC Report
Fri 10 Feb 2017
SRR2031568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665886
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.39691478721582973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.3333904001585857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.16984889305376596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.061.9728281
GTATCAA20400.042.2073331
ATCAACG24900.033.573773
TCAACGC25200.033.174084
CAACGCA25350.033.1651575
TATCAAC25600.033.026822
AACGCAG26050.032.273966
GTGGTAT4950.031.7137991
ACGCAGA29300.028.5319737
CGCAGAG29250.028.4183568
CATAGCG700.00838972527.142432
TGGTATC5150.026.747152
GTACATG19300.024.4015461
GCAGAGT33950.024.204339
TACATGG19500.023.140662
GTAGCAC1652.427665E-623.029943
GCGTGCG1050.002195460322.6186899
ACATGGG19250.022.4541933
TATACAG5350.022.1959115
GAGTACT23450.022.07854312-13
Produced by FastQC (version 0.11.5)