FastQCFastQC Report
Fri 10 Feb 2017
SRR2031567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253111
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.28888103288535494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.21594256215131782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.13270971206860366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.052.805191
GTATCAA36650.045.8799481
ATAGCGC802.2628228E-741.56253
AACGCAG45950.036.2840046
TCAACGC45700.036.2746164
ATCAACG45500.036.2252773
CAACGCA45900.036.1165545
TATCAAC47350.034.9102442
ACGCAGA51300.032.407417
CGCAGAG51400.032.067128
TAGCGCT1051.9018553E-631.6666644
GTACATG45100.029.4900231
TACATGG45700.028.4792122
ACATGGG45200.028.058633
TATAGCG856.407504E-427.9411772
GCAGAGT59350.027.2914929
GTCGTAC950.001226616925.01
CATGGGG24150.024.1925474
AGAGTAC53400.022.86048710-11
GAGTACT33250.022.64285912-13
Produced by FastQC (version 0.11.5)