##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031567_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253111 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02273461808252 34.0 31.0 34.0 30.0 34.0 2 32.182189766110106 34.0 31.0 34.0 30.0 34.0 3 32.28617337171248 34.0 31.0 34.0 30.0 34.0 4 35.52270229851945 37.0 35.0 37.0 33.0 37.0 5 35.513527532676676 37.0 35.0 37.0 33.0 37.0 6 35.644879025082375 37.0 36.0 37.0 33.0 37.0 7 35.66541431684823 37.0 36.0 37.0 35.0 37.0 8 35.71558385490192 37.0 37.0 37.0 35.0 37.0 9 37.52065778689996 39.0 38.0 39.0 35.0 39.0 10-11 37.51356863039268 39.0 38.0 39.0 35.0 39.0 12-13 37.480986520747166 39.0 38.0 39.0 35.0 39.0 14-15 38.95372915886941 41.0 39.0 41.0 36.0 41.0 16-17 38.96003466572395 41.0 39.0 41.0 36.0 41.0 18-19 38.93905847127669 41.0 39.0 41.0 36.0 41.0 20-21 38.902215765403064 41.0 39.0 41.0 35.0 41.0 22-23 38.82141127162718 41.0 39.0 41.0 35.0 41.0 24-25 38.766922882330455 41.0 39.0 41.0 35.0 41.0 26-27 38.65604762866178 41.0 39.0 41.0 34.5 41.0 28-29 38.54672650706921 40.5 39.0 41.0 34.0 41.0 30-31 38.36827344105989 40.0 38.0 41.0 34.0 41.0 32-33 38.24575077547001 40.0 38.0 41.0 34.0 41.0 34-35 38.10630183599059 40.0 38.0 41.0 33.0 41.0 36-37 37.96290512173303 40.0 38.0 41.0 33.0 41.0 38-39 37.80755016913905 40.0 38.0 41.0 33.0 41.0 40-41 37.66288860284524 40.0 38.0 41.0 32.0 41.0 42-43 37.50167503118239 40.0 37.5 41.0 32.0 41.0 44-45 37.6510588447472 40.0 38.0 41.0 32.0 41.0 46-47 37.5058418607769 40.0 38.0 41.0 31.5 41.0 48-49 37.35741406786789 40.0 38.0 41.0 31.0 41.0 50-51 36.298869373902235 39.0 36.0 40.0 29.5 40.5 52-53 36.3683416712486 39.0 36.5 40.0 30.0 40.5 54-55 37.15106762290013 40.0 37.0 41.0 31.0 41.0 56-57 37.193140511894 40.0 37.0 41.0 31.0 41.0 58-59 37.01160910725386 40.0 36.5 41.0 30.5 41.0 60-61 36.845954588220835 40.0 36.0 41.0 30.0 41.0 62-63 36.60071813271131 39.5 35.5 41.0 29.5 41.0 64-65 36.33034822932685 39.0 35.0 41.0 29.0 41.0 66-67 36.03810197181255 39.0 35.0 41.0 29.0 41.0 68-69 35.73407742809695 38.0 35.0 40.5 29.0 41.0 70-71 35.369365523086145 37.0 35.0 40.0 28.0 41.0 72-73 34.998099130883055 37.0 35.0 39.0 28.0 41.0 74-75 34.613926459826786 36.0 35.0 39.0 27.5 41.0 76-77 34.2312692171723 36.0 34.0 39.0 27.0 40.5 78-79 33.82798890122264 35.0 34.0 37.0 26.0 39.5 80-81 33.47955209075653 35.0 34.0 37.0 26.0 39.0 82-83 33.16620993670952 35.0 34.0 36.5 26.0 39.0 84-85 32.86826865297647 35.0 34.0 36.0 25.5 37.0 86-87 32.59891382327663 35.0 34.0 36.0 25.0 37.0 88-89 32.37223278703962 35.0 33.5 35.0 25.0 37.0 90-91 32.17909746223599 35.0 33.0 35.0 24.5 36.0 92-93 32.00231064925613 35.0 33.0 35.0 24.0 36.0 94-95 31.867389640662317 35.0 33.0 35.0 23.5 36.0 96-97 31.74155322234024 35.0 33.0 35.0 23.0 36.0 98-99 31.604871795076413 35.0 33.0 35.0 22.5 35.0 100-101 30.032534627818286 33.5 29.5 34.5 15.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10771920399239576 1101 2 0.1022206424148564 1101 3 0.21553547760366598 1101 4 0.04475700852838571 1101 5 -0.09322402000771035 1101 6 -0.016769420684752845 1101 7 0.005063253130600742 1101 8 0.17395829569775856 1101 9 0.4769260301655791 1101 10-11 0.38464286002349013 1101 12-13 0.5603590523419228 1101 14-15 0.5890900780507948 1101 16-17 0.5514594228025445 1101 18-19 0.6853830802678473 1101 20-21 0.5501292338879367 1101 22-23 0.6532652530162366 1101 24-25 0.48434210831518953 1101 26-27 0.508485361105663 1101 28-29 0.3952781154021068 1101 30-31 0.6795358127167006 1101 32-33 0.6487092255096982 1101 34-35 0.6598683169064543 1101 36-37 0.7260569668174313 1101 38-39 0.6150438361730295 1101 40-41 0.5064410535295494 1101 42-43 0.6287521278097401 1101 44-45 0.63244725487354 1101 46-47 0.6642763845682893 1101 48-49 0.464384947906602 1101 50-51 0.4894138924105107 1101 52-53 0.5844360089093286 1101 54-55 0.5917468179493639 1101 56-57 0.43395161739975663 1101 58-59 0.4020844591678099 1101 60-61 0.30744977234498805 1101 62-63 0.34213010611457406 1101 64-65 0.36895016114960555 1101 66-67 0.459422284467486 1101 68-69 0.322060133835393 1101 70-71 0.275232056328953 1101 72-73 0.22887222839640486 1101 74-75 0.1974942458723703 1101 76-77 0.24797872448859692 1101 78-79 0.2408577001212464 1101 80-81 0.22893197890651606 1101 82-83 0.08630626270183939 1101 84-85 0.2806515313195703 1101 86-87 0.27822774106574144 1101 88-89 0.05272453109233055 1101 90-91 0.1275533141144365 1101 92-93 0.27297913610314595 1101 94-95 0.43045411099191355 1101 96-97 0.479004519661121 1101 98-99 0.25993539335830107 1101 100-101 0.2640720805424053 1102 1 0.24039014416333515 1102 2 0.29324628344049586 1102 3 0.41162522119340395 1102 4 0.11259888887026648 1102 5 0.30316486888117566 1102 6 0.29357245965712764 1102 7 0.3469435950109414 1102 8 0.4400480392875039 1102 9 0.47724654298609437 1102 10-11 0.5306257660063949 1102 12-13 0.5223996506325221 1102 14-15 0.6397631549738705 1102 16-17 0.6482970296401476 1102 18-19 0.6186523110370743 1102 20-21 0.6737082937169987 1102 22-23 0.5888849111358994 1102 24-25 0.6910942450673332 1102 26-27 0.6978443354646373 1102 28-29 0.5988571752311671 1102 30-31 0.710005898186786 1102 32-33 0.7260169178173967 1102 34-35 0.7094943852825253 1102 36-37 0.8438667958772541 1102 38-39 0.8376506737798763 1102 40-41 0.6745820791705768 1102 42-43 0.5514658030234116 1102 44-45 0.7477250326513243 1102 46-47 0.825708008499916 1102 48-49 0.8833699906416399 1102 50-51 0.8665933795900003 1102 52-53 0.8359103678836846 1102 54-55 0.8721634846160313 1102 56-57 0.8507464891946199 1102 58-59 0.9154925788259263 1102 60-61 0.8383044731996918 1102 62-63 0.7647903625248418 1102 64-65 0.8338540073034508 1102 66-67 0.9577556178008209 1102 68-69 0.9171883389636051 1102 70-71 0.8018025691494586 1102 72-73 0.8953145360887191 1102 74-75 0.8473980920262179 1102 76-77 0.7078932544031247 1102 78-79 0.7951205206340646 1102 80-81 0.5473721498466944 1102 82-83 0.5617763481719251 1102 84-85 0.6347540954221316 1102 86-87 0.6604072282452265 1102 88-89 0.6450856422034406 1102 90-91 0.7116986132597347 1102 92-93 0.6918680249920328 1102 94-95 0.7075374443252507 1102 96-97 0.7139297333363395 1102 98-99 0.7154054788283837 1102 100-101 0.5160592600295857 1103 1 0.31677981934556243 1103 2 0.3540912342578437 1103 3 0.3722941405904763 1103 4 0.39699047474529436 1103 5 0.17679642361898829 1103 6 0.25166459751007153 1103 7 0.32905384906231916 1103 8 0.31318246006451744 1103 9 0.3258374070438421 1103 10-11 0.3327717570898301 1103 12-13 0.45272437995103587 1103 14-15 0.4411921939661596 1103 16-17 0.42336425772025876 1103 18-19 0.4488651409854185 1103 20-21 0.44113761577488475 1103 22-23 0.4297622375813006 1103 24-25 0.3812382546915529 1103 26-27 0.4539054776343292 1103 28-29 0.4706793215760854 1103 30-31 0.5782335536959451 1103 32-33 0.48883802035791035 1103 34-35 0.45904546093181153 1103 36-37 0.42410306719563806 1103 38-39 0.46357240614134554 1103 40-41 0.28311385328422745 1103 42-43 0.4299767010490285 1103 44-45 0.4917567713817661 1103 46-47 0.4428131319872861 1103 48-49 0.4095203869334796 1103 50-51 0.47613269032525807 1103 52-53 0.49216523307369187 1103 54-55 0.5488826658487866 1103 56-57 0.43967685505691634 1103 58-59 0.37917481999781444 1103 60-61 0.4352137506747482 1103 62-63 0.555794626200445 1103 64-65 0.6008498851606419 1103 66-67 0.6964630695027481 1103 68-69 0.7115464877145712 1103 70-71 0.6101967910728874 1103 72-73 0.45859163961996074 1103 74-75 0.6006635191014453 1103 76-77 0.6424042585783454 1103 78-79 0.538807715453963 1103 80-81 0.5904626954556349 1103 82-83 0.629130038639687 1103 84-85 0.5205672013594338 1103 86-87 0.4747763457882215 1103 88-89 0.3266461297839385 1103 90-91 0.39793101011770915 1103 92-93 0.43052947853389867 1103 94-95 0.37949581599037785 1103 96-97 0.44941952528312257 1103 98-99 0.36967269275521275 1103 100-101 0.2631321848048991 1104 1 0.13699203703486518 1104 2 0.11365170698727667 1104 3 0.30920039906963126 1104 4 0.0850129712974379 1104 5 0.11749732961416015 1104 6 0.053835582223918266 1104 7 0.08580787209511698 1104 8 0.13980471803633066 1104 9 0.20450020119525192 1104 10-11 0.24775222593390112 1104 12-13 0.31119093134133635 1104 14-15 0.3876328354678975 1104 16-17 0.36608129598462114 1104 18-19 0.3590218237233458 1104 20-21 0.2537970640217324 1104 22-23 0.2774421000203091 1104 24-25 0.35092442361478504 1104 26-27 0.27357262114056624 1104 28-29 0.3775061549599954 1104 30-31 0.29284587670739626 1104 32-33 0.18951201783081473 1104 34-35 0.21952509410250087 1104 36-37 0.2735293344730465 1104 38-39 0.3679030566500501 1104 40-41 0.264102484594126 1104 42-43 0.3023065198981598 1104 44-45 0.37204876738081083 1104 46-47 0.3865131501296659 1104 48-49 0.3481429467431312 1104 50-51 0.16772104773337304 1104 52-53 0.20488103508733246 1104 54-55 0.2880120884554742 1104 56-57 0.3249812858116172 1104 58-59 0.4238267511980567 1104 60-61 0.37818566530601316 1104 62-63 0.27874441676030415 1104 64-65 0.2658524299105025 1104 66-67 0.20910814834183356 1104 68-69 0.1906377952664613 1104 70-71 0.3376404332923002 1104 72-73 0.3177814779601036 1104 74-75 0.38615916733834155 1104 76-77 0.38663201128324687 1104 78-79 0.1338681713254246 1104 80-81 0.2504648468529993 1104 82-83 0.18964250470300215 1104 84-85 0.238234428352726 1104 86-87 0.2710462401931366 1104 88-89 0.15035396681077629 1104 90-91 0.08713155727907207 1104 92-93 -0.038367577102199846 1104 94-95 -0.053010112393778286 1104 96-97 0.18552575293628948 1104 98-99 0.09680566677307922 1104 100-101 0.03406335453891529 1105 1 0.20161522148123368 1105 2 0.14055825290814994 1105 3 0.18973850013004068 1105 4 0.2762274010766248 1105 5 0.19363749505739491 1105 6 0.09594134825356093 1105 7 0.11172611787729636 1105 8 0.1650817630487751 1105 9 0.2707954342146266 1105 10-11 0.2759949178126746 1105 12-13 0.3336572179155297 1105 14-15 0.3888719428925427 1105 16-17 0.3351316022531634 1105 18-19 0.3352847055886272 1105 20-21 0.3081793241835342 1105 22-23 0.2861319006733041 1105 24-25 0.2832818478836714 1105 26-27 0.2978509157107325 1105 28-29 0.37218620398684976 1105 30-31 0.34549152034905006 1105 32-33 0.3344577285037147 1105 34-35 0.2499690677856492 1105 36-37 0.397066851809349 1105 38-39 0.38317281630800437 1105 40-41 0.32238345444201144 1105 42-43 0.3921358365826677 1105 44-45 0.3904431678095719 1105 46-47 0.47009094592177547 1105 48-49 0.5305022371376609 1105 50-51 0.501541066633493 1105 52-53 0.517608235883948 1105 54-55 0.5936401740942188 1105 56-57 0.54053559230708 1105 58-59 0.476709019570805 1105 60-61 0.4016047956317479 1105 62-63 0.45992879445218904 1105 64-65 0.52294930571761 1105 66-67 0.5401551045416184 1105 68-69 0.4325548586714376 1105 70-71 0.433156208025558 1105 72-73 0.5165839478147163 1105 74-75 0.5117072484386753 1105 76-77 0.6071513316552242 1105 78-79 0.6370667106715686 1105 80-81 0.6752189288162214 1105 82-83 0.6991011491194783 1105 84-85 0.6384373881772873 1105 86-87 0.4284173618242235 1105 88-89 0.30654308446178646 1105 90-91 0.3224145440355457 1105 92-93 0.5357303004411449 1105 94-95 0.38262833397451956 1105 96-97 0.26563031568811724 1105 98-99 0.2157114602720931 1105 100-101 0.243782659384717 1106 1 0.043192859466053335 1106 2 0.054186244380453275 1106 3 -0.014939542871360345 1106 4 0.03162569539707505 1106 5 -0.07733267411636291 1106 6 0.0098453759300412 1106 7 -0.015520967453618084 1106 8 0.027037738777202946 1106 9 0.0848703381098872 1106 10-11 0.02803488841551882 1106 12-13 0.02558154756441411 1106 14-15 -0.029251445290725542 1106 16-17 0.005061661404781148 1106 18-19 -0.04998547010070098 1106 20-21 0.10263674139544321 1106 22-23 0.059263069014050984 1106 24-25 0.01571939469248207 1106 26-27 0.11283835045865231 1106 28-29 0.11126228138627425 1106 30-31 0.1735461867270729 1106 32-33 0.14974116654164504 1106 34-35 0.037250244288379974 1106 36-37 0.18745063321109967 1106 38-39 0.2381546742838765 1106 40-41 0.29966791675641247 1106 42-43 0.15514579420339913 1106 44-45 0.25901700644329395 1106 46-47 0.20861845391035416 1106 48-49 0.140431903953548 1106 50-51 0.14100275399937345 1106 52-53 0.2022943217676456 1106 54-55 0.20552241172496366 1106 56-57 0.29297700642514 1106 58-59 0.48558841767176375 1106 60-61 0.33019479009001174 1106 62-63 0.4149065188909944 1106 64-65 0.29460536180480545 1106 66-67 0.34794808299327684 1106 68-69 0.304463901239167 1106 70-71 0.3301214912183852 1106 72-73 0.22683701136119083 1106 74-75 0.29318220656033844 1106 76-77 0.19612641763629313 1106 78-79 0.3563491006126398 1106 80-81 0.27169883667338013 1106 82-83 0.2602470216425985 1106 84-85 0.2714500571180949 1106 86-87 0.341409559247559 1106 88-89 0.22274227611007547 1106 90-91 0.4039401555012816 1106 92-93 0.22239628052029303 1106 94-95 0.20344755898535638 1106 96-97 0.1147593654159742 1106 98-99 0.11692693034983748 1106 100-101 0.06590254737287182 1107 1 0.18860376036030146 1107 2 0.21041181490334537 1107 3 0.3440136796074569 1107 4 0.21378850956638473 1107 5 0.3007582888649267 1107 6 0.17568206410629017 1107 7 0.12527265831157308 1107 8 0.24656322394038455 1107 9 0.32092819642607395 1107 10-11 0.26740168302195855 1107 12-13 0.3237068274118684 1107 14-15 0.351116885524398 1107 16-17 0.24822673032127085 1107 18-19 0.23271451812324528 1107 20-21 0.2934323298352197 1107 22-23 0.32833610778651945 1107 24-25 0.27939175178481435 1107 26-27 0.4006313129063699 1107 28-29 0.37306943230796463 1107 30-31 0.2592794718917162 1107 32-33 0.20708453014008654 1107 34-35 0.2577607259686445 1107 36-37 0.34202456317088803 1107 38-39 0.29692081062921005 1107 40-41 0.16701240473452827 1107 42-43 0.12084888744508504 1107 44-45 0.13819010170087154 1107 46-47 0.25620932075386804 1107 48-49 0.30276205775405884 1107 50-51 0.19438816806716375 1107 52-53 0.11337318735503032 1107 54-55 0.03389683148849798 1107 56-57 0.18976261117175142 1107 58-59 0.19826416852785655 1107 60-61 0.1024127653949094 1107 62-63 0.12344069371273747 1107 64-65 0.22311344303424363 1107 66-67 0.2332836437959429 1107 68-69 0.22525401414949897 1107 70-71 0.15694340211828006 1107 72-73 0.1542702787619632 1107 74-75 0.1322505415669326 1107 76-77 0.2130157314386807 1107 78-79 0.06809707190569725 1107 80-81 0.2505598334060224 1107 82-83 0.10564780977287569 1107 84-85 0.37508559576581746 1107 86-87 0.3768697665192988 1107 88-89 0.3309152522765473 1107 90-91 0.3595115233391013 1107 92-93 0.35192082759155596 1107 94-95 0.18017655886626827 1107 96-97 0.08103629148168778 1107 98-99 0.2204264124033415 1107 100-101 0.13021207512675304 1108 1 0.15637657485971346 1108 2 0.07442532539301538 1108 3 0.010007984838601658 1108 4 -0.15975934681669912 1108 5 0.042618302188202506 1108 6 0.08677262965287724 1108 7 0.1157008046521284 1108 8 0.14407405470854684 1108 9 0.27705757449601975 1108 10-11 0.1153580226999793 1108 12-13 0.1515990768968578 1108 14-15 0.1748319880744731 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2316 58-59 -1.2392429369305802 2316 60-61 -1.3639709231596768 2316 62-63 -1.2408724384146836 2316 64-65 -1.5488615436850068 2316 66-67 -1.2673716086622875 2316 68-69 -1.0357770162918314 2316 70-71 -1.4355567477423037 2316 72-73 -1.2615985095170785 2316 74-75 -1.06286198822432 2316 76-77 -1.0585140660118313 2316 78-79 -1.090898025069599 2316 80-81 -0.8883368930188453 2316 82-83 -0.8554579442532031 2316 84-85 -0.8761678071028243 2316 86-87 -0.7563524116060165 2316 88-89 -0.8389633399848506 2316 90-91 -0.7295374407625772 2316 92-93 -0.8273431709367145 2316 94-95 -0.8650929627994373 2316 96-97 -0.8409403488715306 2316 98-99 -0.7612435718664621 2316 100-101 -0.8684851713659931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 23.0 7 540.0 8 1272.0 9 1055.0 10 1049.0 11 1552.0 12 1855.0 13 2159.0 14 2359.0 15 2523.0 16 2860.0 17 3560.0 18 4530.0 19 5778.0 20 7136.0 21 8829.0 22 9769.0 23 9986.0 24 9561.0 25 8956.0 26 9052.0 27 9707.0 28 10889.0 29 12557.0 30 14987.0 31 18562.0 32 22804.0 33 30485.0 34 43119.0 35 67333.0 36 124462.0 37 261903.0 38 400236.0 39 141510.0 40 153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86906746489338 20.128783483665853 13.988864513997562 23.013284537443212 2 14.43734832748256 21.74212819135735 41.4233854782218 22.397138002938288 3 17.143493273939818 28.01212342721435 31.56982900956101 23.274554289284826 4 11.622194681875747 18.550391784925676 40.69854945012852 29.128864083070056 5 12.268027333572205 39.019368595439666 36.04285653864662 12.669747532341507 6 27.978527041898122 39.73478805947757 19.457174982902554 12.82950991572175 7 24.826292323664862 34.22689610098387 24.265687556808615 16.68112401854265 8 24.512034448664163 35.891712705418755 22.06436620538803 17.531886640529052 9 26.335017408673295 15.971210850435435 21.255818518870235 36.43795322202104 10-11 23.253646325026274 26.957707657182805 30.544460945598594 19.244185072192327 12-13 24.583097586726154 25.131173535305333 29.76324523525849 20.522483642710025 14-15 21.82176997887657 26.289969523849045 27.721486763742398 24.166773733531986 16-17 20.910118896091408 29.49371603952084 28.091845016123873 21.50432004826388 18-19 20.931545569386913 28.466313040105785 30.135478820312006 20.4666625701953 20-21 22.147279849909545 27.75719788590157 29.732481799297904 20.36304046489098 22-23 21.676252143664847 27.79498384420853 29.427839991828336 21.10092402029828 24-25 21.354373235890517 27.81692922654099 29.690107261048702 21.138590276519796 26-27 21.26818773436671 28.1015408850453 29.48597530466176 21.144296075926235 28-29 21.282392381840076 28.163067756966463 29.585407836975335 20.969132024218126 30-31 21.618316334307174 28.00577921668551 29.459800448643414 20.916104000363894 32-33 21.35201909487667 28.129830477906587 29.436697946151618 21.081452481065124 34-35 21.51421542066106 28.215098263441945 29.31982881005753 20.95085750583947 36-37 21.60307426875991 27.984871252426956 29.30095578125162 21.11109869756151 38-39 21.402493474241307 28.314211590194326 29.202999574658588 21.080295360905776 40-41 21.435092342178784 28.142758303135157 29.297005612431782 21.125143742254277 42-43 21.42930674138205 28.061121480858443 29.315120528029837 21.194451249729674 44-45 21.409675599368292 28.28452547300279 29.05919746933831 21.246601458290606 46-47 21.490668409531814 28.160279752200328 28.844298460868128 21.504753377399734 48-49 21.44609070476283 28.29265918102129 28.792535688221975 21.4687144259939 50-51 21.425356572562208 28.424616813674124 28.586533834592466 21.5634927791712 52-53 21.473476810913 28.390022910979155 28.529555641918392 21.60694463618945 54-55 21.40177526172861 28.400676396584178 28.596828213941144 21.600720127746065 56-57 21.37097192507288 28.452627101669364 28.537136773996874 21.63926419926088 58-59 21.329275698641222 28.265373139330833 28.642594311278092 21.762756850749852 60-61 21.35804409984431 28.304356118492297 28.606204877301373 21.731394904362023 62-63 21.252546661867942 28.237243149250148 28.710186088862038 21.800024100019872 64-65 21.289095698625264 27.98830271220985 28.752161620159743 21.97043996900514 66-67 21.297554645997042 28.272236058896617 28.512557945784533 21.917651349321808 68-69 21.297634447387342 28.146868074735597 28.521894708449615 22.03360276942745 70-71 21.318422709560448 27.855872305007296 28.57743647609829 22.24826850933397 72-73 21.220586205052864 28.013519951544595 28.49037315928118 22.275520684121357 74-75 21.210970137521738 28.081071828433394 28.437065830560897 22.270892203483967 76-77 21.14194193491239 27.878615701242747 28.605765969654723 22.373676394190138 78-79 21.2732950233459 27.893299157057918 28.425095622015927 22.40831019758026 80-81 21.18623170652879 27.978646743983575 28.334361441245033 22.500760108242606 82-83 21.18726912460269 27.77802604876982 28.492368194038676 22.542336632588814 84-85 21.296397525837698 27.595959176800775 28.420826247634885 22.686817049726642 86-87 21.153513136505865 27.722085274169643 28.460886545565394 22.66351504375909 88-89 21.2926468604936 27.518312424039053 28.464956416470688 22.724084298996658 90-91 21.407999770171994 27.401004380298318 28.504737409535146 22.686258439994543 92-93 21.441276949927023 27.336125849984562 28.457814192038853 22.764783008049566 94-95 21.295519710544397 27.17117637623483 28.604688650885674 22.9286152623351 96-97 21.33929077312385 26.974665452621515 28.608000408583116 23.078043365671515 98-99 21.41510209390868 26.958545571780952 28.546712940832858 23.079639393477514 100-101 21.61711931345268 26.773925055322312 28.651571967686824 22.957383663538184 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 65.0 1 51.0 2 69.0 3 111.0 4 103.5 5 111.5 6 166.0 7 339.0 8 635.0 9 775.5 10 714.0 11 713.0 12 751.0 13 813.0 14 926.0 15 1025.0 16 1135.0 17 1250.0 18 1408.5 19 1622.0 20 1963.0 21 2384.5 22 2826.5 23 3483.5 24 4397.5 25 5722.5 26 7766.5 27 10328.0 28 12850.5 29 15896.5 30 19377.5 31 22630.5 32 25842.0 33 29164.0 34 32014.0 35 34997.5 36 38512.5 37 41030.0 38 41877.0 39 43035.5 40 45257.0 41 46797.0 42 47295.5 43 48105.0 44 48815.0 45 48987.5 46 49448.0 47 48928.0 48 47950.5 49 47084.5 50 45307.0 51 42894.0 52 40041.5 53 37321.5 54 34210.0 55 30045.0 56 26117.5 57 22360.0 58 19066.5 59 16197.5 60 13261.5 61 10587.0 62 8160.5 63 5901.0 64 4019.5 65 2827.0 66 2010.0 67 1437.0 68 1082.0 69 760.5 70 499.0 71 350.5 72 289.0 73 205.5 74 129.5 75 93.0 76 72.0 77 61.0 78 50.0 79 41.5 80 30.5 81 24.0 82 27.0 83 20.0 84 12.0 85 17.0 86 14.5 87 7.5 88 9.0 89 7.0 90 4.5 91 5.0 92 3.0 93 2.0 94 2.5 95 3.0 96 2.0 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.596027805996436E-4 48-49 1.1970208544973271E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253111.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.861629651906995 #Duplication Level Percentage of deduplicated Percentage of total 1 68.77521952278157 37.73120622687446 2 15.896228977709573 17.441860540740297 3 6.48715727448923 10.676880596601075 4 3.271220874916626 7.178580325970524 5 1.8488439201050852 5.071529521449255 6 1.0809854088482898 3.558277269561009 7 0.6851969020626213 2.6313713075715466 8 0.45564536507741205 1.999795781718795 9 0.3242634626390152 1.601065979725197 >10 1.138175637726066 9.649962908361394 >50 0.026973172637731375 1.0142145449273208 >100 0.009650811993661667 0.8043748349954537 >500 0.0 0.0 >1k 4.3866901315861345E-4 0.6408801615035551 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3620 0.28888103288535494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.21594256215131782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 0.13270971206860366 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.98013902998218E-5 0.0 10-11 0.0 0.0 0.0 1.596027805996436E-4 0.0 12-13 0.0 0.0 0.0 1.596027805996436E-4 0.0 14-15 0.0 0.0 0.0 1.9950347574955452E-4 0.0 16-17 0.0 0.0 0.0 3.192055611992872E-4 0.0 18-19 0.0 0.0 0.0 3.9900695149910903E-4 0.0 20-21 0.0 0.0 0.0 5.187090369488418E-4 0.0 22-23 0.0 0.0 0.0 0.0013965243302468816 0.0 24-25 0.0 0.0 0.0 0.002314240318694832 0.0 26-27 0.0 0.0 0.0 0.004069870905290912 0.0 28-29 0.0 0.0 0.0 0.012608619667371847 0.0 30-31 0.0 0.0 0.0 0.03435449852407328 0.0 32-33 0.0 0.0 0.0 0.08522788484020968 0.0 34-35 0.0 0.0 0.0 0.1562511222070511 0.0 36-37 0.0 0.0 0.0 0.2722025423126922 0.0 38-39 0.0 0.0 0.0 0.43858844108782064 0.0 40-41 0.0 0.0 0.0 0.618859781775118 0.0 42-43 0.0 0.0 0.0 0.7892756507603875 0.0 44-45 0.0 0.0 0.0 0.9938465147939808 0.0 46-47 0.0 0.0 0.0 1.2493705665340102 0.0 48-49 0.0 0.0 0.0 1.5175032379414115 0.0 50-51 0.0 0.0 0.0 1.7825236551271195 0.0 52-53 0.0 0.0 0.0 2.021169712818737 0.0 54-55 0.0 0.0 0.0 2.279047905572611 0.0 56-57 0.0 0.0 0.0 2.5842483227742794 0.0 58-59 0.0 0.0 0.0 2.9334193060311495 0.0 60-61 0.0 0.0 0.0 3.282829693458919 0.0 62-63 0.0 0.0 0.0 3.6293672308358955 0.0 64-65 0.0 0.0 0.0 3.9670069131944414 0.0 66-67 0.0 0.0 0.0 4.350173288719036 0.0 68-69 0.0 0.0 0.0 4.779863874788426 0.0 70-71 0.0 0.0 0.0 5.22607334864988 0.0 72-73 0.0 0.0 0.0 5.654247708303574 0.0 74-75 0.0 0.0 0.0 6.086930846509208 0.0 76-77 0.0 0.0 0.0 6.546666656026481 0.0 78-79 0.0 0.0 0.0 7.052128662185552 0.0 80-81 0.0 0.0 0.0 7.582967510459968 0.0 82-83 0.0 0.0 0.0 8.11871414423782 0.0 84-85 0.0 0.0 0.0 8.654181473149624 0.0 86-87 0.0 0.0 0.0 9.215584253908872 0.0 88-89 0.0 7.98013902998218E-5 0.0 9.842065068457622 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1925 0.0 52.80519 1 GTATCAA 3665 0.0 45.879948 1 ATAGCGC 80 2.2628228E-7 41.5625 3 AACGCAG 4595 0.0 36.284004 6 TCAACGC 4570 0.0 36.274616 4 ATCAACG 4550 0.0 36.225277 3 CAACGCA 4590 0.0 36.116554 5 TATCAAC 4735 0.0 34.910244 2 ACGCAGA 5130 0.0 32.40741 7 CGCAGAG 5140 0.0 32.06712 8 TAGCGCT 105 1.9018553E-6 31.666664 4 GTACATG 4510 0.0 29.490023 1 TACATGG 4570 0.0 28.479212 2 ACATGGG 4520 0.0 28.05863 3 TATAGCG 85 6.407504E-4 27.941177 2 GCAGAGT 5935 0.0 27.291492 9 GTCGTAC 95 0.0012266169 25.0 1 CATGGGG 2415 0.0 24.192547 4 AGAGTAC 5340 0.0 22.860487 10-11 GAGTACT 3325 0.0 22.642859 12-13 >>END_MODULE