FastQCFastQC Report
Fri 10 Feb 2017
SRR2031567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253111
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT44950.358707249397699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44540.35543539239540634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.27347936455748934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.16893954326472274No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14620.11666963261833949TruSeq Adapter, Index 1 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.056.8072051
GTATCAA38600.046.340651
TCAACGC48450.036.7639964
ATCAACG49150.036.24043
CAACGCA49500.036.080115
AACGCAG50150.035.6124736
TATCAAC51950.034.561412
ACGCAGA56900.031.2208397
CGCAGAG56650.031.1909248
GCAGAGT65250.026.4975729
TACATGG40300.025.3407022
GTACATG43050.024.753491
ACATGGG39650.024.5581653
CCTATAC2452.9831426E-1023.2648563
GAGTACT40500.022.51808412-13
AGAGTAC58700.022.37392810-11
CGCACGT753.0355099E-522.16623952-53
CAGAGTA62050.021.81666210-11
AGTACTT44700.020.29602812-13
GTACTTT45650.020.1858114-15
Produced by FastQC (version 0.11.5)