FastQCFastQC Report
Fri 10 Feb 2017
SRR2031564_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031564_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130321
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50230.4443870369567583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.35635894582158517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.19852767488173712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.055.03691
GTATCAA47950.039.3274231
TCAACGC55250.033.3574644
ATCAACG56150.032.9919853
CAACGCA56000.032.9107135
AACGCAG55900.032.7146726
TATCAAC58300.031.8567752
CATGGGA28450.029.7188034
CGCAGAG62250.029.3775088
ACGCAGA62500.029.3359997
GTACATG50700.028.8560161
ACATGGG47450.028.6301383
TACATGG50650.028.0404762
CGCCGTG605.384696E-627.70833494-95
GCAGAGT67450.026.901419
ATGGGAT13700.025.310225
CAGAGTA65700.023.822310-11
CGTATCA2000.023.7594-95
GAGTACT42200.023.6937212-13
ATGGGAG10150.022.4630535
Produced by FastQC (version 0.11.5)