FastQCFastQC Report
Fri 10 Feb 2017
SRR2031564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130321
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT80130.7089136625790373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.48570273400211095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46430.41076826848302384No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT27160.2402857241438494RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.23763161084329143No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT18370.1625202044375005RNA PCR Primer, Index 46 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.055.8634261
ATGGGCG459.626197E-442.221165
GTATCAA52200.040.0993921
AACGCAG59100.035.1217886
TCAACGC59650.034.8775834
CAACGCA59850.034.7610325
ATCAACG60100.034.5374033
TATCAAC61800.033.741062
ACGCAGA65600.031.5693267
CGCAGAG66000.030.9461928
ACATGGG45850.028.2817333
CATGGGA26450.027.834844
GCAGAGT72700.027.8328639
TACATGG48050.027.6788042
GTACATG49350.027.4734571
CGAACTA350.00839428627.14217440-41
AAGATCG700.00839254227.1421745
CAGAGTA71000.024.6525510-11
GAGTACT48100.024.29252812-13
GTACTTT51400.022.91771314-15
Produced by FastQC (version 0.11.5)