##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031564_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1130321 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75329220637323 34.0 31.0 34.0 31.0 34.0 2 32.90007440364286 34.0 33.0 34.0 31.0 34.0 3 32.999254194162546 34.0 33.0 34.0 31.0 34.0 4 36.3741751237038 37.0 37.0 37.0 35.0 37.0 5 36.31017648968744 37.0 37.0 37.0 35.0 37.0 6 36.32944446754506 37.0 37.0 37.0 35.0 37.0 7 36.354520530008735 37.0 37.0 37.0 35.0 37.0 8 36.38646101417208 37.0 37.0 37.0 35.0 37.0 9 38.23973189916847 39.0 39.0 39.0 37.0 39.0 10-11 38.20629361039917 39.0 39.0 39.0 37.0 39.0 12-13 38.21980525886009 39.0 39.0 39.0 37.0 39.0 14-15 39.762082629624686 41.0 40.0 41.0 37.0 41.0 16-17 39.746530852740065 41.0 40.0 41.0 37.0 41.0 18-19 39.78153993423108 41.0 40.0 41.0 37.5 41.0 20-21 39.787182579108055 41.0 40.0 41.0 37.5 41.0 22-23 39.76620756404597 41.0 40.0 41.0 37.0 41.0 24-25 39.71912270938964 41.0 40.0 41.0 37.0 41.0 26-27 39.62024548778621 41.0 40.0 41.0 37.0 41.0 28-29 39.52979065238989 41.0 40.0 41.0 37.0 41.0 30-31 39.38265103452913 41.0 39.0 41.0 36.0 41.0 32-33 38.47848266112016 40.0 38.0 41.0 35.0 41.0 34-35 38.64736212102579 40.0 38.0 41.0 35.0 41.0 36-37 38.83691137296396 40.0 38.0 41.0 35.0 41.0 38-39 38.75442860921809 40.0 38.0 41.0 35.0 41.0 40-41 38.674165568895916 40.0 38.0 41.0 35.0 41.0 42-43 38.47181464380472 40.0 38.0 41.0 35.0 41.0 44-45 38.28797660133714 40.0 38.0 41.0 34.0 41.0 46-47 38.027441762118904 40.0 38.0 41.0 33.5 41.0 48-49 37.84526209811195 40.0 38.0 41.0 33.0 41.0 50-51 37.81300710152249 40.0 38.0 41.0 33.0 41.0 52-53 37.93952824020787 41.0 38.0 41.0 33.0 41.0 54-55 37.86887884061254 41.0 38.0 41.0 33.0 41.0 56-57 37.730632271717504 41.0 38.0 41.0 33.0 41.0 58-59 37.62204807306951 40.0 38.0 41.0 33.0 41.0 60-61 37.44766088571299 40.0 37.5 41.0 32.0 41.0 62-63 37.186133850472565 40.0 37.0 41.0 31.5 41.0 64-65 36.96792327135389 40.0 36.5 41.0 31.0 41.0 66-67 36.685146962676974 39.0 36.0 41.0 31.0 41.0 68-69 36.303420444280874 39.0 36.0 41.0 30.0 41.0 70-71 35.8957601424728 39.0 35.0 41.0 29.0 41.0 72-73 35.521750458498076 37.5 35.0 40.0 29.0 41.0 74-75 35.09382998281019 37.0 35.0 39.5 28.0 41.0 76-77 33.56977088809285 35.5 33.5 38.5 26.0 39.5 78-79 34.235932978330936 36.0 35.0 39.0 27.0 40.0 80-81 34.0230655716385 36.0 35.0 37.5 27.0 39.0 82-83 33.69893994714776 35.0 35.0 37.0 27.0 39.0 84-85 33.3394354347128 35.0 34.5 37.0 26.0 39.0 86-87 33.05183571746433 35.0 34.0 36.0 26.0 37.0 88-89 32.778425774625084 35.0 34.0 36.0 26.0 37.0 90-91 32.57083784163967 35.0 34.0 36.0 25.0 37.0 92-93 32.39989834746059 35.0 34.0 35.0 25.0 36.0 94-95 32.26049768163203 35.0 34.0 35.0 25.0 36.0 96-97 32.14420770736808 35.0 34.0 35.0 24.0 36.0 98-99 32.07105017070372 35.0 34.0 35.0 24.0 36.0 100-101 30.961032308521208 34.5 31.5 35.0 21.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35224890258924546 1101 2 0.34889973739043967 1101 3 0.28919775108839474 1101 4 0.19142394057273293 1101 5 0.24129667693789258 1101 6 0.2912418401903025 1101 7 0.23282371964019433 1101 8 0.20073920190483108 1101 9 0.27701557090943396 1101 10-11 0.30843614525311835 1101 12-13 0.3423837272721926 1101 14-15 0.33822119453336086 1101 16-17 0.3396668579235609 1101 18-19 0.3123608423648747 1101 20-21 0.29296618480251624 1101 22-23 0.3532265721307155 1101 24-25 0.3175245766336374 1101 26-27 0.32867229374319606 1101 28-29 0.37133607704722493 1101 30-31 0.29496689491831063 1101 32-33 0.23014839565416167 1101 34-35 -0.04691082981134542 1101 36-37 0.2534766355760212 1101 38-39 0.22686033035863318 1101 40-41 0.3243841481231371 1101 42-43 0.31548042727166603 1101 44-45 0.31930840360012525 1101 46-47 0.39192364375771405 1101 48-49 0.4633718640596882 1101 50-51 0.4313462723373007 1101 52-53 0.5499293119045277 1101 54-55 0.4736932781354426 1101 56-57 0.47761949292134176 1101 58-59 0.4199503153444937 1101 60-61 0.4954699266313298 1101 62-63 0.5733909763515257 1101 64-65 0.5145088911434712 1101 66-67 0.6804229771110499 1101 68-69 0.6086734737882935 1101 70-71 0.4833506844572142 1101 72-73 0.38604315582305304 1101 74-75 0.4441873627431221 1101 76-77 0.5460426122886517 1101 78-79 0.4211738949419228 1101 80-81 0.327047885769602 1101 82-83 0.43967565312301815 1101 84-85 0.42157192804697985 1101 86-87 0.3413753903824954 1101 88-89 0.43031977134497623 1101 90-91 0.397365653452745 1101 92-93 0.38361333228073846 1101 94-95 0.26754615410352045 1101 96-97 0.20974925773732167 1101 98-99 0.16806912605823499 1101 100-101 0.27280783602457603 1102 1 0.2710877651484864 1102 2 0.31730633079703097 1102 3 0.22638852240238805 1102 4 0.1631311433225946 1102 5 0.16493997676602845 1102 6 0.31087623423228194 1102 7 0.20339816888763096 1102 8 0.15321563542391914 1102 9 0.23448523394781517 1102 10-11 0.23728099708376504 1102 12-13 0.2215631970914771 1102 14-15 0.4033024403018146 1102 16-17 0.418295575177865 1102 18-19 0.37916725869735757 1102 20-21 0.31251594783569203 1102 22-23 0.3486955581210296 1102 24-25 0.3527571166055168 1102 26-27 0.2996601329182411 1102 28-29 0.29885938312504123 1102 30-31 0.4103222149537302 1102 32-33 0.42207850346104436 1102 34-35 0.264371785677433 1102 36-37 0.3185220759176701 1102 38-39 0.3432670279264727 1102 40-41 0.4283858928239397 1102 42-43 0.4047270810917851 1102 44-45 0.48546185914931783 1102 46-47 0.48159996269943406 1102 48-49 0.4739572502374969 1102 50-51 0.5205050401098177 1102 52-53 0.5233421549856061 1102 54-55 0.6109126937908798 1102 56-57 0.4070632718911895 1102 58-59 0.5451033021680942 1102 60-61 0.49483519406531684 1102 62-63 0.5503257714142862 1102 64-65 0.45282265084348694 1102 66-67 0.3823200137237279 1102 68-69 0.293488066778238 1102 70-71 0.4247144155128879 1102 72-73 0.3450264119034685 1102 74-75 0.29870014853553073 1102 76-77 0.28733212845968126 1102 78-79 0.37571076802144887 1102 80-81 0.3868754989147476 1102 82-83 0.53521429694365 1102 84-85 0.2614598245107089 1102 86-87 0.1819598651494374 1102 88-89 0.2890934029266674 1102 90-91 0.2967589142204119 1102 92-93 0.18279045847208408 1102 94-95 0.3059035313234588 1102 96-97 0.4120401540448029 1102 98-99 0.2453860626761184 1102 100-101 0.16922273629018747 1103 1 0.3282156050597038 1103 2 0.3390571363824222 1103 3 0.30421468903766424 1103 4 0.2088369786624682 1103 5 0.2227372288671674 1103 6 0.21578534180147813 1103 7 0.13458361388129703 1103 8 0.10332533757367202 1103 9 0.09676067542897471 1103 10-11 0.15097518516058273 1103 12-13 0.1865545939994533 1103 14-15 0.1991234501724648 1103 16-17 0.263415102160522 1103 18-19 0.27495276172370353 1103 20-21 0.29954513217094103 1103 22-23 0.2366129226223208 1103 24-25 0.2366045568038473 1103 26-27 0.32722120723571635 1103 28-29 0.3179815368505885 1103 30-31 0.35861330489930765 1103 32-33 0.7536239802199702 1103 34-35 0.409603051044634 1103 36-37 0.3241770817463916 1103 38-39 0.37286751865906353 1103 40-41 0.4582300954089433 1103 42-43 0.3601284484234526 1103 44-45 0.392787086571289 1103 46-47 0.351726998297913 1103 48-49 0.4541179600689489 1103 50-51 0.4915637803931574 1103 52-53 0.5556820178451929 1103 54-55 0.548127302757436 1103 56-57 0.5799701497832785 1103 58-59 0.5888288991666855 1103 60-61 0.5193018278128534 1103 62-63 0.7600802269505564 1103 64-65 0.651339635587 1103 66-67 0.6621114014686498 1103 68-69 0.6414029623448485 1103 70-71 0.650664573575618 1103 72-73 0.633270083031185 1103 74-75 0.6411426630818795 1103 76-77 0.7099895675889911 1103 78-79 0.7126357225879545 1103 80-81 0.7907862151094349 1103 82-83 0.6523347045978554 1103 84-85 0.6809859163143557 1103 86-87 0.6145153143682833 1103 88-89 0.4503612899767262 1103 90-91 0.583889655766221 1103 92-93 0.6252992779140598 1103 94-95 0.6244514668359002 1103 96-97 0.7249572640167585 1103 98-99 0.6586138257194776 1103 100-101 0.5942415714620708 1104 1 0.2814720517029059 1104 2 0.24770257688670938 1104 3 0.24570292474685118 1104 4 0.1057377289741126 1104 5 0.12522023513152192 1104 6 0.22633047418404573 1104 7 0.1618363128863649 1104 8 0.18654172055406093 1104 9 0.2579953575461573 1104 10-11 0.20045106464030482 1104 12-13 0.2387902404670328 1104 14-15 0.22299700266683686 1104 16-17 0.24097631488994864 1104 18-19 0.2648153221916161 1104 20-21 0.21958245174725022 1104 22-23 0.27190187493010143 1104 24-25 0.22946209167334786 1104 26-27 0.2755069372019321 1104 28-29 0.2765458307971258 1104 30-31 0.31157574227533047 1104 32-33 0.6406420990310764 1104 34-35 0.47137243801571316 1104 36-37 0.4311807745027849 1104 38-39 0.4411560711142357 1104 40-41 0.4214554162710513 1104 42-43 0.3154704008017859 1104 44-45 0.41276011612117713 1104 46-47 0.3735150450571396 1104 48-49 0.46621938150575204 1104 50-51 0.6511716112319803 1104 52-53 0.5735617014128209 1104 54-55 0.5623573512885685 1104 56-57 0.6472473103593757 1104 58-59 0.6502533152642798 1104 60-61 0.6466039203748153 1104 62-63 0.6572122645523777 1104 64-65 0.7657170807640696 1104 66-67 0.8626139613093358 1104 68-69 0.7644948422008966 1104 70-71 0.6605785660646575 1104 72-73 0.5729315411603508 1104 74-75 0.6454587191239654 1104 76-77 0.5043124446460752 1104 78-79 0.6333590637876725 1104 80-81 0.5730272713475273 1104 82-83 0.5152401433772837 1104 84-85 0.5128478966039722 1104 86-87 0.6003440676905853 1104 88-89 0.5558361345438243 1104 90-91 0.48411467085869475 1104 92-93 0.6097403074953078 1104 94-95 0.5223087272967462 1104 96-97 0.4953281861082175 1104 98-99 0.5401163306784369 1104 100-101 0.42236767399682407 1105 1 0.289125150094236 1105 2 0.24395303738386076 1105 3 0.2771613842616105 1105 4 0.1521672890406336 1105 5 0.1445423833273125 1105 6 0.1571528752630158 1105 7 0.08153289469667868 1105 8 0.08037553363696759 1105 9 0.14435506773995144 1105 10-11 0.2407692811790767 1105 12-13 0.15216526310163658 1105 14-15 0.21133211554847264 1105 16-17 0.1971963045248586 1105 18-19 0.19670753359779525 1105 20-21 0.16597017681848314 1105 22-23 0.1952595391636791 1105 24-25 0.16313281071493435 1105 26-27 0.22695128521080932 1105 28-29 0.2304310738667752 1105 30-31 0.15273076278696607 1105 32-33 0.4329323881088101 1105 34-35 0.4403385724829647 1105 36-37 0.19278894502306088 1105 38-39 0.28809774563796964 1105 40-41 0.2600389374455929 1105 42-43 0.391611132893388 1105 44-45 0.2180452705256144 1105 46-47 0.11001493370529403 1105 48-49 0.19604388265985762 1105 50-51 0.15876643201793428 1105 52-53 0.1325963953201139 1105 54-55 0.16353611992438744 1105 56-57 0.17719835717086596 1105 58-59 0.1983536734634157 1105 60-61 0.10879684422471314 1105 62-63 0.24123660377799894 1105 64-65 0.2988426960611079 1105 66-67 0.08531922856418817 1105 68-69 0.20838339460599542 1105 70-71 0.42901978581493694 1105 72-73 0.38689173339319183 1105 74-75 0.30461485939816413 1105 76-77 0.2641722870051524 1105 78-79 0.39543553649398433 1105 80-81 0.43130914347396754 1105 82-83 0.4286880130186148 1105 84-85 0.5024198475925061 1105 86-87 0.44391344613911343 1105 88-89 0.46492775318350255 1105 90-91 0.49565259605106604 1105 92-93 0.3547013759735691 1105 94-95 0.3062736441783116 1105 96-97 0.2986058851418463 1105 98-99 0.16972104200656446 1105 100-101 0.13948245382194813 1106 1 0.1969458722862143 1106 2 0.17336693685764004 1106 3 0.1369021300427704 1106 4 0.0323954690442676 1106 5 0.015085388226601992 1106 6 0.12822777092623738 1106 7 0.08259894441542315 1106 8 -0.04664882673319681 1106 9 0.07846412235798539 1106 10-11 0.12603000972198686 1106 12-13 -0.010305250416777767 1106 14-15 0.0793602637974331 1106 16-17 0.10642614030783193 1106 18-19 0.06079470017373012 1106 20-21 0.1045192151055474 1106 22-23 0.10719135734549212 1106 24-25 0.17000542911449656 1106 26-27 0.10497932005022648 1106 28-29 0.13528046599162025 1106 30-31 0.06176780234422097 1106 32-33 0.5541785321843022 1106 34-35 0.38153572363520283 1106 36-37 0.1456417621161492 1106 38-39 0.1577731675214764 1106 40-41 0.16440350376749535 1106 42-43 0.1600004905417336 1106 44-45 0.06955815384987574 1106 46-47 0.0718582924423643 1106 48-49 0.14050722869506416 1106 50-51 0.21922506021608967 1106 52-53 0.15602113674634666 1106 54-55 0.10436469005685467 1106 56-57 0.13982707700392893 1106 58-59 0.0188462111153882 1106 60-61 -0.03192455388814608 1106 62-63 0.093251324336876 1106 64-65 0.1485155095250903 1106 66-67 0.07775481194288147 1106 68-69 -0.07100101838620532 1106 70-71 -0.053747633891099156 1106 72-73 -0.21870105829615483 1106 74-75 -0.19770720102643935 1106 76-77 -0.04664074601970469 1106 78-79 -0.09214611837808917 1106 80-81 0.07002886312557877 1106 82-83 0.0020299654773268117 1106 84-85 -0.01720283885278917 1106 86-87 -0.06179955966744899 1106 88-89 -0.08282333554812737 1106 90-91 0.06506774302983587 1106 92-93 -0.09090173598432472 1106 94-95 -0.12353151822415498 1106 96-97 0.06037779586585401 1106 98-99 0.18503133052043808 1106 100-101 0.02547183868857772 1107 1 0.24001098098487006 1107 2 0.2022197309793441 1107 3 0.18568617606359794 1107 4 0.11036120203506528 1107 5 0.11699037703906612 1107 6 0.18090846344854583 1107 7 0.2164453851214816 1107 8 0.09953411710812787 1107 9 0.19474198761032824 1107 10-11 0.19425110513371635 1107 12-13 0.08897032208121658 1107 14-15 0.1930851091055743 1107 16-17 0.17726715643998858 1107 18-19 0.12943187525176114 1107 20-21 0.12831258407324952 1107 22-23 0.1205102228464483 1107 24-25 0.15081742325830305 1107 26-27 0.14801162578763893 1107 28-29 0.24445907984206627 1107 30-31 0.1702717973996002 1107 32-33 0.704690603674031 1107 34-35 0.442430105005883 1107 36-37 0.28186512265867236 1107 38-39 0.07638125155230568 1107 40-41 0.2610915268884 1107 42-43 0.19769540919038775 1107 44-45 0.1971426819015889 1107 46-47 0.30134694607071566 1107 48-49 0.36463605110328245 1107 50-51 0.36041418482590615 1107 52-53 0.24298955612702855 1107 54-55 0.26948875430922214 1107 56-57 0.3209559650180722 1107 58-59 0.21460694631606714 1107 60-61 0.2563681555673867 1107 62-63 0.3581736503776014 1107 64-65 0.32770384341089454 1107 66-67 0.3097805156527329 1107 68-69 0.2036340055081638 1107 70-71 0.3751484946688848 1107 72-73 0.40011687836305043 1107 74-75 0.37170947599905446 1107 76-77 0.21521277667304162 1107 78-79 0.2517238714011967 1107 80-81 0.33294934529457976 1107 82-83 0.3596979166389005 1107 84-85 0.24813125067512232 1107 86-87 0.25480124210597666 1107 88-89 0.33546880417176794 1107 90-91 0.3280106693302116 1107 92-93 0.38295344066825265 1107 94-95 0.45090962290453973 1107 96-97 0.45472118417004737 1107 98-99 0.33701953526616535 1107 100-101 0.3651982965212319 1108 1 0.2352917980316036 1108 2 0.2433563754797845 1108 3 0.14676533988922813 1108 4 0.10849324303077879 1108 5 0.12425855420271859 1108 6 0.25782552460240993 1108 7 0.11126699218534242 1108 8 0.14326048758965726 1108 9 0.10688844602359637 1108 10-11 0.04354844135738034 1108 12-13 0.12462650683401222 1108 14-15 0.11314778715432539 1108 16-17 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20 6416.0 21 9456.0 22 11517.0 23 10280.0 24 8288.0 25 7630.0 26 7871.0 27 9191.0 28 11149.0 29 11945.0 30 11054.0 31 12907.0 32 16013.0 33 20926.0 34 28177.0 35 41665.0 36 72416.0 37 183382.0 38 433246.0 39 206410.0 40 219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.03829075458125 22.945747972060683 15.139289042926043 21.876672230432025 2 13.989654266354426 23.207478229635655 42.962220466575424 19.840647037434497 3 15.685101842750864 30.106491872662723 34.35917761414678 19.849228670439636 4 10.483924478090737 20.982092697561136 42.38884352321155 26.145139301136577 5 10.214266566754047 40.38967691478793 37.930640941820954 11.46541557663708 6 24.287525402076046 42.52119530646604 20.67333085026289 12.517948441195022 7 21.698437877381735 36.224134560005524 25.85416001295207 16.223267549660672 8 22.142028680348325 37.52942748121993 23.611345803537226 16.717198034894512 9 24.798265271546754 16.586438719620354 23.22251820500548 35.39277780382741 10-11 20.973201418004265 28.774834759329433 32.361824649811865 17.890139172854436 12-13 22.4991396249384 27.457686798705854 31.22931450446378 18.813859071891965 14-15 20.173870962319555 28.747851274107088 29.268942185449976 21.80933557812338 16-17 18.533894353904774 31.933627703988517 29.97444973595996 19.558028206146748 18-19 18.469532106366245 29.99895604876845 32.83965351435565 18.691858330509653 20-21 19.679586595312305 29.11080126795839 32.477499754494524 18.73211238223478 22-23 19.304648856386812 29.947200839407568 31.70962054142142 19.0385297627842 24-25 18.966647527560752 30.221990036458667 32.17342684069393 18.63793559528665 26-27 18.937098399481208 30.1021125857168 32.42264807961632 18.53814093518567 28-29 18.519739082968467 29.615480912059493 33.020708276675386 18.84407172829665 30-31 19.133193137170768 30.205269122665158 32.205895493404086 18.45564224675999 32-33 19.438902754173373 29.771498538910627 32.21222113010375 18.57737757681225 34-35 19.161326738156685 29.67572928398216 32.18634352542331 18.976600452437847 36-37 18.871984153174186 30.06384911896709 31.910802329603715 19.15336439825501 38-39 18.37194920734906 29.819228343098995 32.63028821016331 19.178534239388632 40-41 18.614844809571796 29.375460599245702 32.846509973715435 19.163184617467074 42-43 19.109881175347535 29.247001515498695 32.22111240966062 19.422004899493153 44-45 18.884989308346924 29.732615779057454 32.10145613502713 19.280938777568497 46-47 19.105614812403125 29.347622215773566 31.558282078322847 19.988480893500462 48-49 18.808310806357362 29.44015875269782 31.630749576554784 20.120780864390035 50-51 18.624164116864613 29.52469507434422 31.459551373442746 20.39158943534842 52-53 18.73047567903277 29.733367777825947 31.85307536531658 19.683081177824707 54-55 18.906531861303115 29.47746702043048 31.110675639928836 20.505325478337568 56-57 19.215647590374772 29.876468719947695 30.39698457340879 20.510899116268742 58-59 18.589940379768226 29.957419175614714 31.145754170717876 20.306886273899185 60-61 19.19495399756087 30.459900532636542 30.224967166392187 20.120178303410405 62-63 18.523145195037515 30.60935787267511 30.46072752784386 20.406769404443516 64-65 18.346292778777002 30.367214269220867 30.470238100504194 20.816254851497938 66-67 18.498683117450707 30.629396428094317 29.954455415762425 20.917465038692548 68-69 18.729656679510036 30.527079453412192 29.838049695430797 20.905214171646975 70-71 18.59086931942342 29.97489208817672 30.227298263059787 21.206940329340075 72-73 18.630902195040168 29.817458934231954 30.073536632514124 21.478102238213747 74-75 18.706632894549426 29.89075669654903 29.67342905245501 21.72918135644653 76-77 18.552384676565328 29.93631897487528 29.878990127583226 21.632306220976165 78-79 18.65978779479458 29.966443160836615 29.580048499497046 21.793720544871768 80-81 18.63386595489246 29.771808185462362 29.524444825850356 22.069881033794825 82-83 18.865791222139553 29.69032690713523 29.38191894161039 22.061962929114827 84-85 18.914228789874734 29.235456122641267 29.36670202535386 22.48361306213014 86-87 18.576155425012388 29.595424304621698 29.340054143959232 22.48836612640668 88-89 18.845493038242335 29.227438650008224 29.28866035929712 22.63840795245232 90-91 18.695059806072617 29.043500955481633 29.511863896949535 22.749575341496215 92-93 18.926835828052386 28.914264178052072 29.35661639481174 22.8022835990838 94-95 18.802357914256216 28.624833122626224 29.416820531512727 23.155988431604825 96-97 19.03215989086286 28.539105263018204 29.201262296285748 23.227472549833188 98-99 18.804444184923106 28.59948720943195 29.115285865701246 23.480782739943695 100-101 18.727417819441644 28.61292771225038 29.232488967686916 23.427165500621065 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 441.0 1 278.0 2 126.5 3 136.0 4 121.0 5 122.5 6 178.5 7 363.0 8 653.0 9 812.0 10 754.0 11 663.0 12 702.0 13 826.5 14 917.5 15 983.0 16 1110.5 17 1252.0 18 1410.0 19 1657.0 20 1916.0 21 2281.0 22 2759.5 23 3360.0 24 4257.5 25 5780.5 26 7931.0 27 11392.5 28 15929.5 29 22029.5 30 29129.0 31 34599.0 32 40302.0 33 46930.0 34 52891.0 35 56727.0 36 59337.5 37 59839.5 38 55890.0 39 51503.0 40 48139.5 41 45070.0 42 42136.0 43 39946.0 44 38800.5 45 37543.0 46 35699.5 47 33603.5 48 31705.5 49 29952.0 50 27812.5 51 24868.5 52 21939.0 53 19012.0 54 16060.5 55 13229.0 56 10772.0 57 8885.0 58 7120.0 59 5594.0 60 4328.5 61 3128.5 62 2255.5 63 1473.5 64 919.0 65 633.5 66 439.0 67 338.0 68 264.5 69 184.5 70 124.0 71 96.0 72 76.0 73 54.5 74 28.0 75 12.0 76 3.0 77 1.5 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14057953448622118 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0022117610837983194 48-49 8.404692118433613E-4 50-51 3.981169950836975E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.423522167596639E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.00318493596066958 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 8.847044335193277E-5 88-89 2.654113300557983E-4 90-91 8.847044335193277E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.003096465517317647 100-101 4.4235221675966384E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1130321.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.46340243993045 #Duplication Level Percentage of deduplicated Percentage of total 1 63.16906624715652 24.928662830661647 2 15.732586492773404 12.417227843706614 3 7.300461424311207 8.64303141554343 4 4.104606177023206 6.479269016851647 5 2.4425655755004225 4.819597414594674 6 1.6719290252341332 3.9588004784289135 7 1.1472390344519625 3.1691768997962515 8 0.8347332148906385 2.6353130231364963 9 0.6189283547356449 2.1982516869975104 >10 2.747830652202772 18.23394019634286 >50 0.14492806173750283 3.948060815546 >100 0.08195616486348915 5.834639115446054 >500 0.0018111857824168534 0.46602060833097775 >1k 9.055928912084267E-4 1.0615906572447618 >5k 4.5279644560421335E-4 1.2064179973721612 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 8013 0.7089136625790373 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5490 0.48570273400211095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4643 0.41076826848302384 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 2716 0.2402857241438494 RNA PCR Primer, Index 46 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2686 0.23763161084329143 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 1837 0.1625202044375005 RNA PCR Primer, Index 46 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.423522167596639E-5 0.0 12-13 0.0 0.0 0.0 1.7694088670386555E-4 0.0 14-15 0.0 0.0 0.0 1.7694088670386555E-4 0.0 16-17 0.0 0.0 0.0 7.519987684914285E-4 0.0 18-19 0.0 0.0 0.0 0.0010616453202231932 0.0 20-21 0.0 0.0 0.0 0.0015924679803347899 0.0 22-23 0.0 0.0 0.0 0.00415811083754084 0.0 24-25 0.0 0.0 0.0 0.008271986453405714 0.0 26-27 0.0 0.0 0.0 0.014243741379661175 0.0 28-29 0.0 0.0 0.0 0.029195246306137818 0.0 30-31 0.0 0.0 0.0 0.07427093719394756 0.0 32-33 0.0 0.0 0.0 0.1755695948319106 0.0 34-35 0.0 0.0 0.0 0.34158438178181244 0.0 36-37 0.0 0.0 0.0 0.6081458276011859 0.0 38-39 0.0 0.0 0.0 0.9426083386931676 0.0 40-41 0.0 0.0 0.0 1.2586689975679475 0.0 42-43 0.0 0.0 0.0 1.5795955308270835 0.0 44-45 0.0 0.0 0.0 1.9826668707384894 0.0 46-47 0.0 0.0 0.0 2.471067953262834 0.0 48-49 0.0 0.0 0.0 2.9285928510573545 0.0 50-51 0.0 0.0 0.0 3.370060363383499 0.0 52-53 0.0 0.0 0.0 3.790958497630319 0.0 54-55 0.0 0.0 0.0 4.278784522272877 0.0 56-57 0.0 0.0 0.0 4.849241941006139 0.0 58-59 0.0 0.0 0.0 5.441949676242412 0.0 60-61 0.0 0.0 0.0 6.014441915172769 0.0 62-63 0.0 0.0 0.0 6.559685257550731 0.0 64-65 0.0 0.0 0.0 7.145934650422314 0.0 66-67 0.0 0.0 0.0 7.820566016202477 0.0 68-69 0.0 0.0 0.0 8.552039641836256 0.0 70-71 0.0 0.0 0.0 9.278824333972384 0.0 72-73 0.0 0.0 0.0 9.991763401723935 0.0 74-75 0.0 0.0 0.0 10.722352322924195 0.0 76-77 0.0 0.0 0.0 11.521550072943882 0.0 78-79 0.0 0.0 0.0 12.378917139467461 0.0 80-81 0.0 0.0 0.0 13.240619257715286 0.0 82-83 0.0 0.0 0.0 14.108691247884451 0.0 84-85 0.0 0.0 0.0 14.971499246674174 0.0 86-87 0.0 0.0 0.0 15.901367841524664 0.0 88-89 0.0 0.0 0.0 16.868659433913024 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 55.863426 1 ATGGGCG 45 9.626197E-4 42.22116 5 GTATCAA 5220 0.0 40.099392 1 AACGCAG 5910 0.0 35.121788 6 TCAACGC 5965 0.0 34.877583 4 CAACGCA 5985 0.0 34.761032 5 ATCAACG 6010 0.0 34.537403 3 TATCAAC 6180 0.0 33.74106 2 ACGCAGA 6560 0.0 31.569326 7 CGCAGAG 6600 0.0 30.946192 8 ACATGGG 4585 0.0 28.281733 3 CATGGGA 2645 0.0 27.83484 4 GCAGAGT 7270 0.0 27.832863 9 TACATGG 4805 0.0 27.678804 2 GTACATG 4935 0.0 27.473457 1 CGAACTA 35 0.008394286 27.142174 40-41 AAGATCG 70 0.008392542 27.142174 5 CAGAGTA 7100 0.0 24.65255 10-11 GAGTACT 4810 0.0 24.292528 12-13 GTACTTT 5140 0.0 22.917713 14-15 >>END_MODULE