FastQCFastQC Report
Fri 10 Feb 2017
SRR2031561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337004
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA122120.5225493837408922No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT100710.4309363612556931No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT41130.17599456398020713No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC28790.12319191580331057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.047.3838651
GTATCAA38250.039.2418331
TCAACGC39250.036.9108284
CAACGCA40350.035.7868655
ATCAACG40800.035.741423
AACGCAG41650.035.126056
CGCAGAG46300.030.982728
TATCAAC48400.030.3254132
ACGCAGA47650.030.2046177
GTACATG51550.027.6430641
ACATGGG47050.026.5515423
GCAGAGT56300.024.635889
TACATGG55100.023.7931022
CATGGGG25850.022.050294
GAGTACT28950.021.32987812-13
GCCGTAT7550.020.7615994-95
CGCCGTA8450.020.51775294-95
AGAGTAC48250.020.37823710-11
ATCGCCG600.004765645619.79166858-59
CAGAGTA56950.019.266910-11
Produced by FastQC (version 0.11.5)