##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031561_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2337004 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23090589489791 34.0 31.0 34.0 30.0 34.0 2 32.388307422665946 34.0 31.0 34.0 31.0 34.0 3 32.490541308444485 34.0 31.0 34.0 31.0 34.0 4 35.71893415672373 37.0 35.0 37.0 35.0 37.0 5 35.70493375278776 37.0 37.0 37.0 35.0 37.0 6 35.82790615677166 37.0 37.0 37.0 35.0 37.0 7 35.85698569621618 37.0 37.0 37.0 35.0 37.0 8 35.903710477175046 37.0 37.0 37.0 35.0 37.0 9 37.727270043183495 39.0 39.0 39.0 35.0 39.0 10-11 37.73545210021035 39.0 39.0 39.0 35.0 39.0 12-13 37.7185396345064 39.0 39.0 39.0 35.0 39.0 14-15 39.266970659870495 41.0 40.0 41.0 36.0 41.0 16-17 39.28448346686612 41.0 40.0 41.0 37.0 41.0 18-19 39.24258815988334 41.0 40.0 41.0 36.5 41.0 20-21 39.2176945353966 41.0 40.0 41.0 36.0 41.0 22-23 39.15919741686365 41.0 40.0 41.0 36.0 41.0 24-25 39.11999637142256 41.0 39.5 41.0 36.0 41.0 26-27 39.034366008787316 41.0 39.0 41.0 36.0 41.0 28-29 38.94979191306476 41.0 39.0 41.0 36.0 41.0 30-31 38.811376660031385 41.0 39.0 41.0 35.0 41.0 32-33 38.752767218199025 41.0 39.0 41.0 35.0 41.0 34-35 38.65979818605359 40.5 39.0 41.0 35.0 41.0 36-37 38.536740630311286 40.0 38.0 41.0 35.0 41.0 38-39 38.41366039595995 40.0 38.0 41.0 34.0 41.0 40-41 38.323582458566605 40.0 38.0 41.0 34.0 41.0 42-43 38.21363206909359 40.0 38.0 41.0 34.0 41.0 44-45 38.37426380100334 40.0 38.5 41.0 34.0 41.0 46-47 38.30980969651742 40.0 38.0 41.0 34.0 41.0 48-49 38.243213961122876 40.0 38.0 41.0 34.0 41.0 50-51 37.237281151422934 39.0 37.0 40.0 32.5 40.5 52-53 37.327374921052765 39.5 37.5 40.0 33.0 40.5 54-55 38.15210650045957 40.0 38.0 41.0 34.0 41.0 56-57 38.22629978382579 41.0 38.5 41.0 34.0 41.0 58-59 38.08247332909999 41.0 38.0 41.0 34.0 41.0 60-61 37.970260213504126 40.0 38.0 41.0 33.0 41.0 62-63 37.77326739706051 40.0 37.0 41.0 33.0 41.0 64-65 37.5698041167238 40.0 37.0 41.0 33.0 41.0 66-67 37.34422427175991 40.0 36.5 41.0 32.5 41.0 68-69 37.09937894843141 39.0 36.0 41.0 32.0 41.0 70-71 36.78955363362664 39.0 35.5 41.0 32.0 41.0 72-73 36.45745856660921 38.5 35.0 41.0 31.5 41.0 74-75 36.07515477080912 37.0 35.0 40.0 31.0 41.0 76-77 35.681423737400536 37.0 35.0 39.0 31.0 41.0 78-79 35.216260648248785 36.5 35.0 39.0 30.0 41.0 80-81 34.782495023542964 36.0 35.0 39.0 30.0 39.5 82-83 34.38685941487477 35.5 35.0 37.0 29.0 39.0 84-85 33.980769823243776 35.0 34.0 37.0 29.0 39.0 86-87 33.61670519177545 35.0 34.0 36.5 29.0 38.0 88-89 33.29435679185829 35.0 34.0 36.0 28.5 37.0 90-91 32.99980658997588 35.0 34.0 36.0 27.0 37.0 92-93 32.76390926160161 35.0 34.0 35.5 26.0 36.0 94-95 32.554824681515306 35.0 34.0 35.0 26.0 36.0 96-97 32.35400217543487 35.0 34.0 35.0 25.0 36.0 98-99 32.152887842725136 35.0 33.5 35.0 25.0 36.0 100-101 30.526807613508574 34.0 30.0 35.0 19.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07159986806486529 1101 2 -0.1230082869360416 1101 3 0.04092250008605447 1101 4 -0.13245581686443586 1101 5 -0.27042876683105277 1101 6 -0.13767798750346572 1101 7 -0.1592181961328336 1101 8 -0.03347787624389298 1101 9 0.23588852216093414 1101 10-11 0.228363362367908 1101 12-13 0.3612217100931687 1101 14-15 0.39149858709399865 1101 16-17 0.23882254793605284 1101 18-19 0.3535205385077731 1101 20-21 0.42711275993292475 1101 22-23 0.34964288217089035 1101 24-25 0.42468950853889 1101 26-27 0.3991631732504999 1101 28-29 0.4000912620259882 1101 30-31 0.46015928767366887 1101 32-33 0.4455685312668436 1101 34-35 0.45652617861436084 1101 36-37 0.47177384753031504 1101 38-39 0.5445773690548208 1101 40-41 0.47029294121813336 1101 42-43 0.47391793020752715 1101 44-45 0.4829454821678496 1101 46-47 0.5618706215573326 1101 48-49 0.4661784698150413 1101 50-51 0.5493388877539545 1101 52-53 0.6600906083613296 1101 54-55 0.5936786904634133 1101 56-57 0.47491031851513554 1101 58-59 0.6165314122440719 1101 60-61 0.6118541212246171 1101 62-63 0.4821964200835822 1101 64-65 0.4640987765000375 1101 66-67 0.46194069204121746 1101 68-69 0.36679056955815525 1101 70-71 0.5012333787063028 1101 72-73 0.4836322494760168 1101 74-75 0.4921336723749832 1101 76-77 0.4152754849580589 1101 78-79 0.478760566493996 1101 80-81 0.48988081731805977 1101 82-83 0.5505672002776976 1101 84-85 0.37158300415789824 1101 86-87 0.3947089213724837 1101 88-89 0.353159790532402 1101 90-91 0.42437826440226445 1101 92-93 0.402288124335648 1101 94-95 0.4303029838189545 1101 96-97 0.31847902630990177 1101 98-99 0.2351258595542447 1101 100-101 0.2354561197775098 1102 1 0.20942302798493984 1102 2 0.24387179843143514 1102 3 0.3383497106706628 1102 4 0.1825713175848449 1102 5 0.10278943430707699 1102 6 0.2109580961434787 1102 7 0.2221122386628096 1102 8 0.15844271221621398 1102 9 0.35843679240536375 1102 10-11 0.4051466224884379 1102 12-13 0.4092564670671166 1102 14-15 0.3967932143081754 1102 16-17 0.3600007945155781 1102 18-19 0.415326721655191 1102 20-21 0.49627431968792024 1102 22-23 0.5150375044392703 1102 24-25 0.4755482415451979 1102 26-27 0.5066887677579643 1102 28-29 0.4255490106685116 1102 30-31 0.5352174008065589 1102 32-33 0.4808446267237514 1102 34-35 0.5074281873579309 1102 36-37 0.4626567175322478 1102 38-39 0.5813875987646853 1102 40-41 0.4820536380062137 1102 42-43 0.5044524286510992 1102 44-45 0.6121930613493376 1102 46-47 0.7583525851292734 1102 48-49 0.6544322357408845 1102 50-51 0.6753808775835992 1102 52-53 0.6781255376888637 1102 54-55 0.6773080223459758 1102 56-57 0.6190260172290749 1102 58-59 0.7291884600819358 1102 60-61 0.6937653501356564 1102 62-63 0.6405629502162355 1102 64-65 0.711984260281568 1102 66-67 0.6507197617215255 1102 68-69 0.6210553840982058 1102 70-71 0.617060795815469 1102 72-73 0.5775941788170442 1102 74-75 0.5847100287602345 1102 76-77 0.5547277077947541 1102 78-79 0.5558730594312422 1102 80-81 0.4862608299972848 1102 82-83 0.4904137381437863 1102 84-85 0.5151216930416922 1102 86-87 0.598349178973649 1102 88-89 0.5942545430401509 1102 90-91 0.5446679533115315 1102 92-93 0.6558637118740336 1102 94-95 0.5737779768124014 1102 96-97 0.5215057426143943 1102 98-99 0.5647153732623522 1102 100-101 0.5515900639918989 1103 1 0.20353816267120095 1103 2 0.22453198835560784 1103 3 0.2587270606312799 1103 4 0.12176798260972532 1103 5 0.06883174490042876 1103 6 0.06918953142483275 1103 7 0.12945520383068754 1103 8 0.14753016251637519 1103 9 0.24855523922445855 1103 10-11 0.2196246522452867 1103 12-13 0.23844041416815998 1103 14-15 0.3244122857212801 1103 16-17 0.23231147114719874 1103 18-19 0.3092197982982299 1103 20-21 0.3166467323815638 1103 22-23 0.2732363418616117 1103 24-25 0.2566479671997186 1103 26-27 0.29955287037697076 1103 28-29 0.23491280515447954 1103 30-31 0.30796154568381695 1103 32-33 0.2658471726879199 1103 34-35 0.23905382117214202 1103 36-37 0.2633202148304363 1103 38-39 0.23367254212292465 1103 40-41 0.286942662521227 1103 42-43 0.2332783941544747 1103 44-45 0.3481188762363203 1103 46-47 0.31995896955082515 1103 48-49 0.33420437194758534 1103 50-51 0.2642115305163273 1103 52-53 0.2931287617836844 1103 54-55 0.31679295237955074 1103 56-57 0.2788394004347552 1103 58-59 0.35575024254238485 1103 60-61 0.26938375769455547 1103 62-63 0.3136323003767316 1103 64-65 0.40648789244446704 1103 66-67 0.3819447630896846 1103 68-69 0.3021040714990164 1103 70-71 0.2731542755298477 1103 72-73 0.25193740334015047 1103 74-75 0.2757144473167443 1103 76-77 0.25419061642795526 1103 78-79 0.2570292126935314 1103 80-81 0.23582450646471642 1103 82-83 0.2678920082206133 1103 84-85 0.1316340552443549 1103 86-87 0.2176668605758465 1103 88-89 0.24193087485303977 1103 90-91 0.1284028113680762 1103 92-93 0.1622791846738565 1103 94-95 0.1710123934891854 1103 96-97 0.13252019036621476 1103 98-99 0.1470178290470372 1103 100-101 0.15353927319639382 1104 1 0.14473270084472745 1104 2 0.14515865487761204 1104 3 0.24833256151656258 1104 4 0.20516499316438797 1104 5 0.11437348693354465 1104 6 0.134917340106945 1104 7 0.20213196688891344 1104 8 0.15496347709527925 1104 9 0.3054276517722556 1104 10-11 0.25496215185133764 1104 12-13 0.3786563639042342 1104 14-15 0.4186592231440116 1104 16-17 0.32117529385741506 1104 18-19 0.3975424292085208 1104 20-21 0.46603930593844467 1104 22-23 0.4280700241926141 1104 24-25 0.43706103474716684 1104 26-27 0.3776141689376402 1104 28-29 0.3969321971287201 1104 30-31 0.4090932436104495 1104 32-33 0.3951980835175988 1104 34-35 0.39140176694240125 1104 36-37 0.33818551102896777 1104 38-39 0.356314999562521 1104 40-41 0.38527772921828074 1104 42-43 0.3783454373563089 1104 44-45 0.3173758237699147 1104 46-47 0.42114310754207906 1104 48-49 0.35447904422301946 1104 50-51 0.2543595148287423 1104 52-53 0.3396843020119249 1104 54-55 0.3281293060539454 1104 56-57 0.3248753190374387 1104 58-59 0.4657178330324925 1104 60-61 0.38231937834467544 1104 62-63 0.40528836278402025 1104 64-65 0.4410179958831435 1104 66-67 0.38966701467489173 1104 68-69 0.35902543671797105 1104 70-71 0.3164050583850937 1104 72-73 0.30909436136175117 1104 74-75 0.3154800324781206 1104 76-77 0.31226605055677936 1104 78-79 0.30409869138761536 1104 80-81 0.4341112404716654 1104 82-83 0.24463613118766148 1104 84-85 0.2746860308398382 1104 86-87 0.33670399899126835 1104 88-89 0.32619392145029735 1104 90-91 0.39862297697536064 1104 92-93 0.29866558890254424 1104 94-95 0.3227647699540839 1104 96-97 0.27009269585951046 1104 98-99 0.30219598835427774 1104 100-101 0.2880591469475853 1105 1 0.1879855409421225 1105 2 0.16316776567353486 1105 3 0.1848746620375934 1105 4 0.14474387016817758 1105 5 0.15810330152458363 1105 6 0.1417659474697004 1105 7 0.15378238027069102 1105 8 0.17441750573380688 1105 9 0.3153069713943779 1105 10-11 0.2914501175166393 1105 12-13 0.3612811976920298 1105 14-15 0.3451140711524161 1105 16-17 0.3408909004395255 1105 18-19 0.32281930182321616 1105 20-21 0.33916817231818186 1105 22-23 0.3575310943274559 1105 24-25 0.305130706143224 1105 26-27 0.29146651116228384 1105 28-29 0.34913387174068333 1105 30-31 0.3517670903176153 1105 32-33 0.32283778748086434 1105 34-35 0.3203265031661786 1105 36-37 0.3002526275210684 1105 38-39 0.40151830381790177 1105 40-41 0.2578561507055923 1105 42-43 0.35062330660279173 1105 44-45 0.4142296347028207 1105 46-47 0.3850125811978131 1105 48-49 0.40319143466757623 1105 50-51 0.3892255106523592 1105 52-53 0.39902599324452837 1105 54-55 0.440809807080079 1105 56-57 0.3822871877955194 1105 58-59 0.41492809621745863 1105 60-61 0.3693034557932009 1105 62-63 0.40579320938132213 1105 64-65 0.5036713361856684 1105 66-67 0.3950104118201878 1105 68-69 0.3853328541261618 1105 70-71 0.5064377608759258 1105 72-73 0.4692281101410316 1105 74-75 0.5216229275057032 1105 76-77 0.3955169920857742 1105 78-79 0.3585834061627011 1105 80-81 0.37465036392548967 1105 82-83 0.36891445944920065 1105 84-85 0.4661784618727083 1105 86-87 0.4029694006609361 1105 88-89 0.3863111335588769 1105 90-91 0.39014612563209994 1105 92-93 0.43340248673973747 1105 94-95 0.4445807902794954 1105 96-97 0.4082340720323856 1105 98-99 0.47333408681441114 1105 100-101 0.25341808716390446 1106 1 0.29767292569821535 1106 2 0.256616567158801 1106 3 0.20710904335312819 1106 4 0.2515732607709822 1106 5 0.23833896253245968 1106 6 0.2911137393883152 1106 7 0.21490900357704845 1106 8 0.22335972655309888 1106 9 0.2706117377565249 1106 10-11 0.1887158954521979 1106 12-13 0.23266916547981253 1106 14-15 0.28802632653476934 1106 16-17 0.34614225155986134 1106 18-19 0.30572937556401314 1106 20-21 0.36472299895965676 1106 22-23 0.2824340954399176 1106 24-25 0.29884766591423784 1106 26-27 0.3379011558050138 1106 28-29 0.3465012555874054 1106 30-31 0.20767360063766915 1106 32-33 0.29167736621856477 1106 34-35 0.19556983380270054 1106 36-37 0.2871334856275851 1106 38-39 0.3229375643900667 1106 40-41 0.3315039318908788 1106 42-43 0.3226987319409105 1106 44-45 0.26757391850684087 1106 46-47 0.3362738782427357 1106 48-49 0.39820961902152874 1106 50-51 0.25920196947213014 1106 52-53 0.3042249237592429 1106 54-55 0.3046759207436125 1106 56-57 0.3288134701274785 1106 58-59 0.42018524027378845 1106 60-61 0.4599796269554446 1106 62-63 0.42866710896941385 1106 64-65 0.45213661256227056 1106 66-67 0.47712702821218755 1106 68-69 0.40187704209535013 1106 70-71 0.40600836222859016 1106 72-73 0.41424986862719493 1106 74-75 0.4818090621899387 1106 76-77 0.5339147140847373 1106 78-79 0.5560734472313413 1106 80-81 0.4489302309371723 1106 82-83 0.45589484675396363 1106 84-85 0.5529319409679658 1106 86-87 0.4363840391259828 1106 88-89 0.43715037696208725 1106 90-91 0.5360845014721036 1106 92-93 0.3346509628150187 1106 94-95 0.4650634096134283 1106 96-97 0.41921496474348885 1106 98-99 0.4706377483652915 1106 100-101 0.46813927096895114 1107 1 0.31532162151904686 1107 2 0.21107343735672401 1107 3 0.31442996465520423 1107 4 0.23790184674307824 1107 5 0.31087928602384807 1107 6 0.1939971132770495 1107 7 0.20848057734688297 1107 8 0.25779924872394844 1107 9 0.302259868709676 1107 10-11 0.2602221132445308 1107 12-13 0.25063054850630095 1107 14-15 0.3493296996402222 1107 16-17 0.2798385769567986 1107 18-19 0.3066065160156839 1107 20-21 0.34646312294458426 1107 22-23 0.3845591044278649 1107 24-25 0.37666046760610783 1107 26-27 0.3927909185419409 1107 28-29 0.4032925253771964 1107 30-31 0.3082342411304779 1107 32-33 0.305494970321746 1107 34-35 0.34508487853312886 1107 36-37 0.38356630274037684 1107 38-39 0.31483059428349947 1107 40-41 0.36006756622381175 1107 42-43 0.2866402401624768 1107 44-45 0.3143102950112464 1107 46-47 0.45336570488099426 1107 48-49 0.5058095551164712 1107 50-51 0.422756545407978 1107 52-53 0.38418913361069684 1107 54-55 0.3610560762749273 1107 56-57 0.3722176246608555 1107 58-59 0.35252430841618576 1107 60-61 0.39912777500381935 1107 62-63 0.44181105760168293 1107 64-65 0.5077736956485879 1107 66-67 0.3953296922077172 1107 68-69 0.33887979121473677 1107 70-71 0.4405809163318679 1107 72-73 0.41928912651622596 1107 74-75 0.44544668983687785 1107 76-77 0.5016440061207064 1107 78-79 0.41581500058429555 1107 80-81 0.4532945900662 1107 82-83 0.43842207633910846 1107 84-85 0.37841942943905593 1107 86-87 0.45263185896676816 1107 88-89 0.4413143593330773 1107 90-91 0.5548682166229639 1107 92-93 0.5439181702986247 1107 94-95 0.5338004081768091 1107 96-97 0.4625535357774666 1107 98-99 0.46714555653158385 1107 100-101 0.46195370054066487 1108 1 0.020172692366358547 1108 2 -0.01565343507758854 1108 3 -0.0042918211838909315 1108 4 -0.11425152588775234 1108 5 0.030902241433686584 1108 6 0.07474605394244804 1108 7 0.03665805121332255 1108 8 0.12374191115087285 1108 9 0.17881308786363803 1108 10-11 0.12943841080763718 1108 12-13 0.21123131359588143 1108 14-15 0.1264788376552488 1108 16-17 0.08979407605513501 1108 18-19 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2565.0 11 3082.0 12 3415.0 13 3628.0 14 3463.0 15 3564.0 16 3814.0 17 4402.0 18 5008.0 19 6240.0 20 7874.0 21 8956.0 22 9254.0 23 9593.0 24 9626.0 25 9917.0 26 11018.0 27 12568.0 28 15054.0 29 17881.0 30 21609.0 31 27584.0 32 35285.0 33 47750.0 34 68557.0 35 106856.0 36 190961.0 37 414359.0 38 810729.0 39 453596.0 40 1212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.47708262373535 23.21117122606551 15.381702384762713 21.93004376543643 2 12.948758324761103 23.436759201096788 43.59924073728586 20.015241736856247 3 15.670747675228627 29.598408903022843 33.504820702061274 21.226022719687258 4 11.23588149613779 19.36817395263337 43.837708450648776 25.558236100580057 5 10.757790744046652 40.14212213586284 38.33493652556864 10.765150594521874 6 24.865896677968887 42.684137468314134 20.692604719546907 11.757361134170075 7 22.485584106830796 35.01003849372958 27.305173632565456 15.19920376687417 8 21.48708346241598 38.8249228499395 24.13949655199563 15.548497135648889 9 24.102868458932893 17.932232893054525 23.63693001809154 34.327968629921045 10-11 21.24275354256989 28.280760323901884 33.20781650352331 17.26866963000491 12-13 22.103620704115183 26.694070699065986 32.0214043279344 19.180904268884436 14-15 19.657497376983525 28.736878499138214 29.226158791341394 22.37946533253687 16-17 19.386209865280506 31.345688753635 29.968198599574496 19.29990278151 18-19 19.33640250508771 30.64752991436899 31.279984972212286 18.73608260833101 20-21 20.00172015109944 30.357906961220433 30.63084188131471 19.009531006365414 22-23 20.15644817039252 29.806303284033746 30.499519898125975 19.537728647447757 24-25 19.872345105100376 30.160303533926342 30.184565366597578 19.782785994375704 26-27 19.647484557150953 30.42823204410433 30.354633539352093 19.569649859392623 28-29 19.919028807824034 29.893380584714446 30.714517390642037 19.473073216819483 30-31 20.210662882904778 29.918305659725014 30.27970854992118 19.591322907449022 32-33 19.735995317081187 30.320893759702592 30.30899818742287 19.634112735793348 34-35 20.025104792289618 30.004056475727044 30.331377267646953 19.63946146433639 36-37 20.25289644348063 29.64746744121961 30.137582135075508 19.962053980224255 38-39 19.646286441957308 30.201766877591997 30.447380492288417 19.70456618816228 40-41 19.948896108008373 29.6809076920707 30.664303527079973 19.705892672840953 42-43 19.985502806156944 29.676585919407927 30.125387033997374 20.21252424043776 44-45 19.78727892635186 29.870915924833675 30.24975567008015 20.09204947873431 46-47 19.9443689203651 29.578524289442505 30.34260118622013 20.134505603972265 48-49 19.634158943619347 29.51865394864395 30.404961749012678 20.442225358724023 50-51 19.280262250299955 29.780287068400398 30.255831825705048 20.6836188555946 52-53 19.691707844744812 29.561438491333348 30.339400360461514 20.407453303460326 54-55 19.793782980260197 29.346269839503915 30.165245759100113 20.69470142113578 56-57 19.590360136311276 29.748558410683078 29.99213950853315 20.668941944472497 58-59 19.73961105757628 29.127378472608516 30.400910738706482 20.73209973110872 60-61 19.95152768245155 29.245777927637267 29.999392384437513 20.803302005473675 62-63 19.430540127445227 29.411075034531393 30.165759237040245 20.99262560098314 64-65 19.823521910959503 28.98717760003834 30.14451408726729 21.04478640173487 66-67 19.996371422556404 29.018521149300557 29.68886660014275 21.296240828000297 68-69 19.622623666882898 29.23435304346933 29.723911469556747 21.41911182009102 70-71 19.903004017109087 28.836343455124595 30.0239965357355 21.236655992030823 72-73 20.02341459407001 28.71907793054697 29.641048966967965 21.61645850841505 74-75 19.809230108292496 29.14336047349512 29.479581549710655 21.567827868501723 76-77 19.93571684087832 29.12827705900375 29.76715059109869 21.16885550901924 78-79 20.20950755753948 28.979774959734772 29.327292550633207 21.483424932092543 80-81 20.12619576175308 29.084331905294132 29.233625616387478 21.55584671656531 82-83 20.338347730684244 28.964969679127634 29.29520017937496 21.40148241081316 84-85 20.719198597862906 28.579732854543682 29.154057930581207 21.54701061701221 86-87 20.1724515661933 28.904293702535384 29.34119924484511 21.58205548642621 88-89 20.783297760722704 28.3141791798388 29.319290852732816 21.58323220670568 90-91 20.91984010296944 28.549330681505037 29.031892970658156 21.49893624486736 92-93 20.890571860381925 28.581508632419972 29.013857058011027 21.51406244918708 94-95 21.154456731781373 28.04364049013181 29.091948494739416 21.709954283347397 96-97 21.397759695747204 27.840281830925406 28.887798223708643 21.874160249618743 98-99 21.205804525794562 28.119014772760337 28.76101196232441 21.914168739120687 100-101 21.901802478729177 27.710970969668857 28.81678850357124 21.570438048030727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 69.0 1 45.5 2 37.0 3 66.5 4 77.0 5 89.0 6 120.5 7 186.0 8 320.5 9 405.0 10 370.5 11 376.0 12 415.0 13 448.5 14 525.5 15 598.0 16 656.0 17 745.0 18 868.0 19 939.5 20 1055.5 21 1331.5 22 1593.5 23 1936.0 24 2474.0 25 3432.0 26 5575.5 27 9891.5 28 16854.0 29 28510.5 30 45118.5 31 64481.0 32 84998.0 33 110381.5 34 135998.5 35 142391.5 36 142791.5 37 144786.0 38 135159.0 39 122214.0 40 108590.0 41 94302.0 42 82474.5 43 72967.5 44 66313.5 45 62065.5 46 58223.5 47 54843.5 48 52532.0 49 50419.0 50 48151.5 51 45960.5 52 43514.5 53 40980.5 54 38319.0 55 35063.0 56 31318.0 57 27381.5 58 24240.0 59 20845.5 60 16873.0 61 13398.5 62 10371.5 63 7665.5 64 5637.5 65 4190.0 66 3007.0 67 2264.0 68 1748.5 69 1265.0 70 893.0 71 660.5 72 494.0 73 389.5 74 282.0 75 207.5 76 168.0 77 129.5 78 103.0 79 82.0 80 69.5 81 60.0 82 49.5 83 33.0 84 26.5 85 24.5 86 25.5 87 26.5 88 20.0 89 13.0 90 8.0 91 5.0 92 2.0 93 1.5 94 1.5 95 1.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.278982834432461E-5 48-49 2.3534405589378536E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2337004.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.5683028773424 #Duplication Level Percentage of deduplicated Percentage of total 1 63.56765564116319 24.51696595970985 2 15.762967344315015 12.159017975623982 3 6.987968274885725 8.085422307691575 4 3.8060104547297384 5.871654558893929 5 2.3239527808888365 4.4815457362981395 6 1.5653609339774721 3.622398876840163 7 1.112875237898133 3.004519646796477 8 0.8061109942237662 2.487226638238226 9 0.6214032280454016 2.1569821117391985 >10 3.203454622933745 21.57909136151702 >50 0.16643844486314413 4.340225567272037 >100 0.07052748246144853 4.902147182295793 >500 0.00415231288800557 1.0982666899745765 >1k 8.97797381190372E-4 0.7300569188855066 >5k 0.0 0.0 >10k+ 2.24449345297593E-4 0.9644784682234983 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 12212 0.5225493837408922 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 10071 0.4309363612556931 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 4113 0.17599456398020713 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 2879 0.12319191580331057 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.278982834432462E-5 0.0 2 0.0 0.0 0.0 4.278982834432462E-5 0.0 3 0.0 0.0 0.0 4.278982834432462E-5 0.0 4 0.0 0.0 0.0 4.278982834432462E-5 0.0 5 0.0 0.0 0.0 4.278982834432462E-5 0.0 6 0.0 0.0 0.0 4.278982834432462E-5 0.0 7 0.0 0.0 0.0 4.278982834432462E-5 0.0 8 0.0 0.0 0.0 4.278982834432462E-5 0.0 9 0.0 0.0 0.0 4.278982834432462E-5 0.0 10-11 0.0 0.0 0.0 6.418474251648693E-5 0.0 12-13 0.0 0.0 0.0 2.1394914172162305E-4 0.0 14-15 0.0 0.0 0.0 3.209237125824346E-4 0.0 16-17 0.0 0.0 0.0 5.776626826483823E-4 0.0 18-19 0.0 0.0 0.0 8.130067385421676E-4 0.0 20-21 0.0 0.0 0.0 0.0014120643353627122 0.0 22-23 0.0 0.0 0.0 0.0025032049581429896 0.0 24-25 0.0 0.0 0.0 0.004086428606883001 0.0 26-27 0.0 0.0 0.0 0.006782187792575452 0.0 28-29 0.0 0.0 0.0 0.014270407752832259 0.0 30-31 0.0 0.0 0.0 0.04246890463174218 0.0 32-33 0.0 0.0 0.0 0.12764205795112032 0.0 34-35 0.0 0.0 0.0 0.2590710157107134 0.0 36-37 0.0 0.0 0.0 0.4752238335920692 0.0 38-39 0.0 0.0 0.0 0.7785181368966421 0.0 40-41 0.0 0.0 0.0 1.079437604728105 0.0 42-43 0.0 0.0 0.0 1.402286859586034 0.0 44-45 0.0 0.0 0.0 1.7983709056552748 0.0 46-47 0.0 0.0 0.0 2.2890418672796455 0.0 48-49 0.0 0.0 0.0 2.783991811738448 0.0 50-51 0.0 0.0 0.0 3.2492670102404615 0.0 52-53 0.0 0.0 0.0 3.699030895967658 0.0 54-55 0.0 0.0 0.0 4.208614961720219 0.0 56-57 0.0 0.0 0.0 4.81802769700009 0.0 58-59 0.0 0.0 0.0 5.460966262787741 0.0 60-61 0.0 0.0 0.0 6.073759394506814 0.0 62-63 0.0 0.0 0.0 6.669800308429083 0.0 64-65 0.0 0.0 0.0 7.3033892967234975 0.0 66-67 0.0 0.0 0.0 8.032335417483239 0.0 68-69 0.0 0.0 0.0 8.823840267282385 0.0 70-71 0.0 0.0 0.0 9.608092241177165 0.0 72-73 0.0 0.0 0.0 10.358026772739798 0.0 74-75 0.0 0.0 0.0 11.144674977021863 0.0 76-77 0.0 0.0 0.0 11.977600380658313 0.0 78-79 0.0 0.0 0.0 12.882155956943162 0.0 80-81 0.0 0.0 0.0 13.800147539328131 0.0 82-83 0.0 0.0 0.0 14.719123287765019 0.0 84-85 0.0 0.0 0.0 15.63675115660906 0.0 86-87 0.0 0.0 0.0 16.604485914444304 0.0 88-89 0.0 0.0 0.0 17.63901559432504 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2045 0.0 47.383865 1 GTATCAA 3825 0.0 39.241833 1 TCAACGC 3925 0.0 36.910828 4 CAACGCA 4035 0.0 35.786865 5 ATCAACG 4080 0.0 35.74142 3 AACGCAG 4165 0.0 35.12605 6 CGCAGAG 4630 0.0 30.98272 8 TATCAAC 4840 0.0 30.325413 2 ACGCAGA 4765 0.0 30.204617 7 GTACATG 5155 0.0 27.643064 1 ACATGGG 4705 0.0 26.551542 3 GCAGAGT 5630 0.0 24.63588 9 TACATGG 5510 0.0 23.793102 2 CATGGGG 2585 0.0 22.05029 4 GAGTACT 2895 0.0 21.329878 12-13 GCCGTAT 755 0.0 20.76159 94-95 CGCCGTA 845 0.0 20.517752 94-95 AGAGTAC 4825 0.0 20.378237 10-11 ATCGCCG 60 0.0047656456 19.791668 58-59 CAGAGTA 5695 0.0 19.2669 10-11 >>END_MODULE