FastQCFastQC Report
Fri 10 Feb 2017
SRR2031561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337004
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT132550.5671791747040227No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA126150.5397936845636551No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT95290.40774427429306925No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT45900.19640531210044998RNA PCR Primer, Index 46 (95% over 21bp)
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT45000.19255422754946075No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC28950.12387655305681976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.042.5379371
GTATCAA35100.040.804371
TCAACGC37650.036.9647144
CAACGCA39000.035.5633745
ATCAACG39150.035.4271163
AACGCAG40150.034.7813576
CGCAGAG43600.031.593398
TATCAAC44700.031.4534952
ACGCAGA44100.031.4506027
GTACATG46300.026.1035791
GCAGAGT52600.025.8264669
ACATGGG42250.025.295343
TACATGG51550.022.666852
AGAGTAC46400.021.7021410-11
GAGTACT28500.019.91625812-13
CATGGGG22100.019.773354
TAATACT26350.017.8459344
CAGAGTA55200.017.51096510-11
CATGGGA24350.016.9709034
GTATATA18550.016.9296251
Produced by FastQC (version 0.11.5)