FastQCFastQC Report
Fri 10 Feb 2017
SRR2031560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199937
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.042.486111
GTATCAA33700.038.338281
TCAACGC34600.036.6546254
ATCAACG35150.035.9459463
CAACGCA36400.034.8420335
AACGCAG37500.033.826
CGCAGAG41450.030.5971058
ACGCAGA42100.030.1247027
TATCAAC42950.029.528522
GCAGAGT50600.024.8764829
GTACATG51150.023.6803511
ACATGGG44900.023.2739413
TCGCCGG1750.021.71428594-95
CGCCGTA5050.021.63366394-95
TACATGG55300.020.4430392
AGAGTAC51600.019.69961210-11
CATGGGG23150.019.6976264
GTATAAT28750.019.660871
GTATATA24400.018.6885241
GCCGTAT5100.018.6274594-95
Produced by FastQC (version 0.11.5)