FastQCFastQC Report
Fri 10 Feb 2017
SRR2031560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199937
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT41990.19086910216065278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.045.919121
GTATCAA36300.039.1740151
TCAACGC34800.038.7631874
ATCAACG35200.038.32273
CAACGCA36200.037.1328475
AACGCAG37050.036.280956
TATCAAC42650.032.4081572
ACGCAGA41700.032.3491367
CGCAGAG42100.031.8161358
GCAGAGT52150.024.7739359
ACATGGG40250.023.365753
GTACATG48400.021.1264551
CAGAGTA53500.019.39894510-11
TACATGG52250.018.9085182
TATAACT19750.018.5184254
CTATAAC14650.018.4806673
CATGGGG21350.018.2430064
AGAGTAC52950.018.12031710-11
GAGTACT35250.017.71931812-13
CATGGGA21550.016.5308194
Produced by FastQC (version 0.11.5)