##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031559_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151658 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.167871131097602 33.0 31.0 34.0 22.0 34.0 2 30.314055308655 33.0 31.0 34.0 22.0 34.0 3 30.328396787508737 34.0 31.0 34.0 22.0 34.0 4 33.438723971040105 37.0 35.0 37.0 23.0 37.0 5 33.418843714146306 37.0 35.0 37.0 22.0 37.0 6 33.61796937846998 37.0 35.0 37.0 24.0 37.0 7 33.62733255087104 37.0 35.0 37.0 24.0 37.0 8 33.673713223173195 37.0 35.0 37.0 25.0 37.0 9 35.25505413496156 39.0 37.0 39.0 25.0 39.0 10-11 35.254216724472165 39.0 37.0 39.0 25.0 39.0 12-13 35.233957325033955 39.0 37.0 39.0 24.5 39.0 14-15 36.424013240317024 40.0 37.5 41.0 24.0 41.0 16-17 36.39285102005829 40.0 37.5 41.0 24.0 41.0 18-19 36.44329675981484 40.0 37.5 41.0 24.0 41.0 20-21 36.4549215999156 40.0 37.5 41.0 23.5 41.0 22-23 36.338495166756786 40.0 37.0 41.0 23.0 41.0 24-25 36.23691793377204 40.0 37.0 41.0 23.0 41.0 26-27 36.0448014611824 40.0 36.0 41.0 21.5 41.0 28-29 35.85422463701222 40.0 35.5 41.0 19.5 41.0 30-31 35.52091877777631 40.0 35.0 41.0 18.0 41.0 32-33 35.20611177781588 40.0 35.0 41.0 17.5 41.0 34-35 34.97839546875206 40.0 35.0 41.0 16.0 41.0 36-37 34.71158461802213 40.0 35.0 41.0 15.0 41.0 38-39 34.42777499373591 39.0 35.0 41.0 14.0 41.0 40-41 34.172889000250564 39.0 34.5 41.0 12.5 41.0 42-43 33.848168246976755 39.0 33.5 41.0 11.0 41.0 44-45 33.6438532751322 39.0 33.0 41.0 9.5 41.0 46-47 33.167004048582996 39.5 32.5 41.0 8.0 41.0 48-49 32.75934339105092 39.0 31.0 41.0 8.0 41.0 50-51 31.737570718326765 38.0 29.0 40.0 8.0 40.5 52-53 31.76000276938902 38.5 28.5 39.5 8.0 40.5 54-55 32.302420577879175 39.0 29.0 41.0 7.0 41.0 56-57 32.275231112107505 40.0 28.0 41.0 7.0 41.0 58-59 32.04729061440874 39.0 27.5 41.0 7.0 41.0 60-61 31.86296799377547 39.0 26.5 41.0 7.0 41.0 62-63 31.58750280235794 39.0 26.0 41.0 7.0 41.0 64-65 31.34189096519801 38.5 25.0 41.0 7.0 41.0 66-67 31.021640797056534 37.5 24.0 41.0 7.0 41.0 68-69 30.66291919977845 37.0 23.0 40.0 7.0 41.0 70-71 30.284442627490805 36.5 22.5 39.5 7.0 41.0 72-73 29.93353136662754 36.0 21.5 39.0 7.0 41.0 74-75 29.519593427316725 35.5 20.5 39.0 7.0 41.0 76-77 29.16117844096586 35.0 20.0 38.5 7.0 40.5 78-79 28.767189993274343 35.0 19.5 37.0 7.0 39.5 80-81 28.379696422213136 35.0 19.0 37.0 7.0 39.0 82-83 28.04561249653826 35.0 18.0 36.0 7.0 39.0 84-85 27.6999861530549 35.0 17.5 36.0 7.0 37.5 86-87 27.39234989252133 35.0 17.0 35.5 7.0 37.0 88-89 27.121335504885995 35.0 15.0 35.0 7.0 36.5 90-91 26.879518390061847 34.5 14.0 35.0 7.0 36.0 92-93 26.669509686267787 34.0 12.0 35.0 7.0 36.0 94-95 26.46849160611376 34.0 11.5 35.0 7.0 36.0 96-97 26.294425615529676 34.0 10.5 35.0 7.0 35.5 98-99 26.093822943728654 34.0 10.0 35.0 7.0 35.0 100-101 24.8105803848132 31.5 10.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34114374827016647 1101 2 -0.14207846651000366 1101 3 -0.4481872728677665 1101 4 -0.5811313183465003 1101 5 -0.5955872466329595 1101 6 -0.7906058735729147 1101 7 -1.2183881746035183 1101 8 -0.37071051605607863 1101 9 -0.2842990036087869 1101 10-11 0.250991776004831 1101 12-13 -3.292303663258167E-4 1101 14-15 0.3176083337519344 1101 16-17 0.16453969778855537 1101 18-19 0.588896690479288 1101 20-21 0.18582258045960742 1101 22-23 0.043833554710687395 1101 24-25 0.1363225077957253 1101 26-27 -0.3354545170475305 1101 28-29 -0.5437739568840669 1101 30-31 -0.1411137792270054 1101 32-33 0.29288923653749777 1101 34-35 -0.1880744103191958 1101 36-37 0.13282456389673314 1101 38-39 0.1557756624971276 1101 40-41 -0.390663809224435 1101 42-43 0.0044931968070400785 1101 44-45 0.3794484514455476 1101 46-47 0.8703930576162762 1101 48-49 0.2030457775606891 1101 50-51 0.15189240307767804 1101 52-53 0.6102835397529098 1101 54-55 0.8097547756779733 1101 56-57 0.5043109987948711 1101 58-59 1.3100861027862152 1101 60-61 0.9386544115314983 1101 62-63 0.7531146921466068 1101 64-65 1.2663899012676758 1101 66-67 0.6408939482608176 1101 68-69 0.302455189007528 1101 70-71 0.6454478623147182 1101 72-73 0.36490574904038553 1101 74-75 0.4210668887174549 1101 76-77 0.545323057040445 1101 78-79 0.8671315829243369 1101 80-81 0.6717389794429138 1101 82-83 0.046923390416015565 1101 84-85 0.03151637871141588 1101 86-87 0.11522691137876961 1101 88-89 0.01842298106170759 1101 90-91 0.23909807621355128 1101 92-93 0.44177877045834535 1101 94-95 0.8501495637726251 1101 96-97 0.2599871706214252 1101 98-99 -0.3368955989850697 1101 100-101 -0.49164064334418 1102 1 -0.4904800314560056 1102 2 -0.6762783190173778 1102 3 -0.8010633790624553 1102 4 -1.0974476900279129 1102 5 -0.6912730873409245 1102 6 -1.078400858823649 1102 7 -0.5522746052819869 1102 8 -1.161086622250771 1102 9 -0.2634656702754512 1102 10-11 0.02947628927916668 1102 12-13 -0.22663822741647266 1102 14-15 -0.31656386388818447 1102 16-17 -0.13533124616424885 1102 18-19 0.004272796673980395 1102 20-21 0.2715987604006145 1102 22-23 -0.13338805295892797 1102 24-25 -0.15124202022787614 1102 26-27 -0.040699723537208854 1102 28-29 0.022413358750149825 1102 30-31 0.06740391988803651 1102 32-33 -0.1111022826365442 1102 34-35 -0.19346710943424483 1102 36-37 -0.15344940365488924 1102 38-39 -0.03822323130818006 1102 40-41 -0.08917966468165872 1102 42-43 -0.31068010702777116 1102 44-45 -0.7731603243656551 1102 46-47 -0.20229315182324825 1102 48-49 0.32717004009756323 1102 50-51 0.1404617246116011 1102 52-53 -0.09348674048307615 1102 54-55 0.16028021815584736 1102 56-57 -0.8318800041549856 1102 58-59 -0.724482481284582 1102 60-61 -0.31816712239180944 1102 62-63 -0.585703523192624 1102 64-65 -0.4205570013871913 1102 66-67 -0.6986156387598257 1102 68-69 -1.0257619938833322 1102 70-71 -0.3292940255908867 1102 72-73 -0.6157358438799712 1102 74-75 -1.2545876098076185 1102 76-77 -1.1575253942869885 1102 78-79 -1.1951379598485268 1102 80-81 -1.2371843243918939 1102 82-83 -1.4635393676960824 1102 84-85 -1.1078641522620352 1102 86-87 -0.5755916726920276 1102 88-89 -0.9332731841300301 1102 90-91 -0.9710328234914627 1102 92-93 -1.34149925314048 1102 94-95 -1.5722324421270777 1102 96-97 -1.4527248500275398 1102 98-99 -1.5093697877756327 1102 100-101 -1.3580215430491904 1103 1 0.42488189275547583 1103 2 0.8053040121224733 1103 3 0.7622828126023187 1103 4 0.9853750919099085 1103 5 0.8825110439653301 1103 6 1.0988172033501655 1103 7 0.932787039071691 1103 8 1.0432852104396488 1103 9 1.292089885280106 1103 10-11 0.9761520324150865 1103 12-13 1.029318205531112 1103 14-15 1.1155517098203163 1103 16-17 1.0355332875321395 1103 18-19 1.0819522460348452 1103 20-21 0.9105821958442277 1103 22-23 0.9942876145397435 1103 24-25 1.2675992171974357 1103 26-27 1.1705550983370827 1103 28-29 1.4225721969620864 1103 30-31 1.4299332293200067 1103 32-33 1.3918475698708406 1103 34-35 1.4824651195953322 1103 36-37 1.3800200767172441 1103 38-39 1.4411656197620815 1103 40-41 1.4740530711174529 1103 42-43 1.4668329403967775 1103 44-45 1.63679353691564 1103 46-47 2.372663356761578 1103 48-49 2.5090019741418885 1103 50-51 2.1807385569238313 1103 52-53 1.6974897363341164 1103 54-55 1.2503263568745524 1103 56-57 1.412163562897426 1103 58-59 1.5320412309913394 1103 60-61 1.631496291873372 1103 62-63 1.5799042220611348 1103 64-65 1.6542104140881868 1103 66-67 2.0540883927052676 1103 68-69 1.3656229240502604 1103 70-71 1.8671358965027487 1103 72-73 2.278901475536113 1103 74-75 2.527236760512327 1103 76-77 2.907636091228479 1103 78-79 2.26016042907818 1103 80-81 2.2454569281608627 1103 82-83 2.2642043733219985 1103 84-85 2.243054840249876 1103 86-87 2.22366708231894 1103 88-89 2.1947050323437622 1103 90-91 1.560545725786202 1103 92-93 1.6407638131933844 1103 94-95 1.719273495396557 1103 96-97 1.695751059510318 1103 98-99 1.8701022642627905 1103 100-101 1.6222749549464197 1104 1 0.5592185705704118 1104 2 0.4498599392870979 1104 3 0.4666677995960029 1104 4 0.4446838990448043 1104 5 0.5919127533670405 1104 6 0.4494303583111474 1104 7 0.9799813906138723 1104 8 0.6718619477120384 1104 9 0.6060995471158535 1104 10-11 0.7052490223816434 1104 12-13 0.8579135232568618 1104 14-15 0.9786137685345437 1104 16-17 1.0617317267740631 1104 18-19 0.8887155310589989 1104 20-21 0.8567917833581618 1104 22-23 1.0141686996382262 1104 24-25 0.9639946092449989 1104 26-27 0.6022719322278292 1104 28-29 0.7107550286231827 1104 30-31 1.2402268004831427 1104 32-33 1.0295287292911226 1104 34-35 0.9380578360576166 1104 36-37 1.0592285494039757 1104 38-39 0.8643354451058229 1104 40-41 0.7313923501958612 1104 42-43 0.9261417475316733 1104 44-45 1.3882799731846802 1104 46-47 1.156851390949619 1104 48-49 1.4392323717635875 1104 50-51 1.0921097943820222 1104 52-53 1.0769954962746588 1104 54-55 1.3865210800258012 1104 56-57 1.851684187200668 1104 58-59 1.7118071172789655 1104 60-61 1.504777599937288 1104 62-63 1.5028882428712471 1104 64-65 1.5471870027169565 1104 66-67 1.2799338033332859 1104 68-69 1.4204352614712938 1104 70-71 1.282223224633558 1104 72-73 1.5306666186056042 1104 74-75 1.5503694249493378 1104 76-77 1.590839361388273 1104 78-79 1.5951660032141923 1104 80-81 1.5757244866892925 1104 82-83 1.7378201295464493 1104 84-85 1.310501885957791 1104 86-87 1.2954805345671758 1104 88-89 1.1760316767138832 1104 90-91 1.3530020617207974 1104 92-93 1.087159205240951 1104 94-95 1.200693042033496 1104 96-97 0.7996157938098314 1104 98-99 1.0285935314497117 1104 100-101 1.4297814581050972 1105 1 -0.04577337789979552 1105 2 0.2600048668233299 1105 3 0.35829420861371375 1105 4 0.08622979276461251 1105 5 0.23635719781148623 1105 6 0.5848570893900487 1105 7 0.5635562698409231 1105 8 -0.1572845901301534 1105 9 0.21060840379862356 1105 10-11 0.17529733156038674 1105 12-13 0.5600874363003356 1105 14-15 0.21355740782600918 1105 16-17 0.4457896977885554 1105 18-19 0.2263966904792909 1105 20-21 0.1803827656448007 1105 22-23 -0.039615519363387364 1105 24-25 -0.29365434405612945 1105 26-27 -0.6892739614919847 1105 28-29 -0.6979406235507284 1105 30-31 -0.2675026681158954 1105 32-33 -0.452596874573608 1105 34-35 -0.08819015105994055 1105 36-37 -0.5253930286958592 1105 38-39 0.03945621805268473 1105 40-41 0.2603778574422364 1105 42-43 -0.2137938402300037 1105 44-45 -0.3227506226285186 1105 46-47 -0.3412967571985348 1105 48-49 -0.06906070392079044 1105 50-51 -0.002737226551953853 1105 52-53 0.11572335456773075 1105 54-55 0.07653718308537805 1105 56-57 0.4554684062022716 1105 58-59 0.24828054723066373 1105 60-61 0.536339596716676 1105 62-63 0.8870267291836491 1105 64-65 1.0228713827491624 1105 66-67 0.9096439482608218 1105 68-69 0.8157653741927113 1105 70-71 0.19591082527768222 1105 72-73 0.24129463792927552 1105 74-75 0.35173818501374754 1105 76-77 0.3067813903737786 1105 78-79 0.021413990331740962 1105 80-81 0.004146386850319317 1105 82-83 0.19611320523082654 1105 84-85 -0.23700213980710316 1105 86-87 0.22309728174914056 1105 88-89 -0.004725167086441218 1105 90-91 0.10356566880614437 1105 92-93 0.0906213630509356 1105 94-95 0.6246866008096639 1105 96-97 0.06612142988068825 1105 98-99 0.05870625286678077 1105 100-101 0.7536139862854512 1106 1 -0.9314722154234509 1106 2 -0.4859805346365249 1106 3 -0.6954500465903948 1106 4 -1.1329105154267936 1106 5 -1.4226857867789455 1106 6 -1.053531664327167 1106 7 -1.1324696831193393 1106 8 -1.2744513919684906 1106 9 -1.0590861082316536 1106 10-11 -0.729163333484216 1106 12-13 -1.5136351914368902 1106 14-15 -1.215504500797941 1106 16-17 -1.3984925406542672 1106 18-19 -1.2327273971119581 1106 20-21 -1.1184277236766391 1106 22-23 -1.4207725888416292 1106 24-25 -1.3079825408660781 1106 26-27 -1.4180655267798912 1106 28-29 -0.7921012074923439 1106 30-31 -0.5968619544094835 1106 32-33 -1.3113154471607942 1106 34-35 -0.7627778774724803 1106 36-37 -1.102386810799132 1106 38-39 -1.1657343739992214 1106 40-41 -0.65568053671835 1106 42-43 -1.2280950027063469 1106 44-45 -1.4936887140045698 1106 46-47 -1.989742283016323 1106 48-49 -1.4783334803468513 1106 50-51 -0.8502365506936123 1106 52-53 -1.3506729687629004 1106 54-55 -1.768826428701587 1106 56-57 -1.4937881496236258 1106 58-59 -1.5961638972137848 1106 60-61 -1.3936144449161922 1106 62-63 -1.1458740548120296 1106 64-65 -1.1286830914330572 1106 66-67 -1.359942426435044 1106 68-69 -0.6005633633039089 1106 70-71 -0.7351786583665465 1106 72-73 -0.19941906847786228 1106 74-75 -0.660859814445562 1106 76-77 -0.8564789502588219 1106 78-79 -0.7599593538153222 1106 80-81 -0.669197760216452 1106 82-83 -0.44984516189542134 1106 84-85 -0.6235931103396766 1106 86-87 -0.6334044754825428 1106 88-89 -0.850494293877464 1106 90-91 -0.49535295784970756 1106 92-93 -0.3776403292983481 1106 94-95 -0.3157567622614401 1106 96-97 -0.7576147150233403 1106 98-99 -0.36419182769553515 1106 100-101 -0.4370862029548839 1107 1 1.3401476424583088 1107 2 1.2626538734458457 1107 3 1.438402131105743 1107 4 1.3896882180773389 1107 5 1.6647604354862438 1107 6 1.3486049432041298 1107 7 1.1738579622618204 1107 8 1.237319285268427 1107 9 1.5577263827046792 1107 10-11 1.2296755508392678 1107 12-13 1.323001343585105 1107 14-15 1.6191742829793085 1107 16-17 1.4071583512101924 1107 18-19 1.4568602666382304 1107 20-21 1.726764909377934 1107 22-23 1.2799331683972213 1107 24-25 1.1818178168464968 1107 26-27 1.4547289818487101 1107 28-29 1.5490792439989391 1107 30-31 1.4246974790511402 1107 32-33 1.5761742806876171 1107 34-35 1.4326830400119306 1107 36-37 1.2932481290181386 1107 38-39 1.7289337750799092 1107 40-41 1.8398480561177308 1107 42-43 1.7486295102728207 1107 44-45 1.5093808465890461 1107 46-47 1.9256151878591083 1107 48-49 1.4404560976489904 1107 50-51 1.279651784976128 1107 52-53 1.3728732196646156 1107 54-55 1.6889973253468469 1107 56-57 1.5688115506712492 1107 58-59 1.1356241822564144 1107 60-61 1.091110261421118 1107 62-63 1.1482236877316048 1107 64-65 1.3533104310689943 1107 66-67 1.3270832418590572 1107 68-69 1.1548627497139279 1107 70-71 1.2126388115420887 1107 72-73 1.4000878682456843 1107 74-75 1.5616229041699903 1107 76-77 1.4496069974377939 1107 78-79 1.1546246844695887 1107 80-81 1.1863637035047212 1107 82-83 1.3982961829104958 1107 84-85 1.2152912131485039 1107 86-87 1.3341838650211528 1107 88-89 1.684261833158839 1107 90-91 1.571017226323928 1107 92-93 1.4898610000389176 1107 94-95 1.4311374224922737 1107 96-97 1.560725305279263 1107 98-99 1.4593930323615076 1107 100-101 1.4356841359052694 1108 1 0.06307703095061257 1108 2 -0.002332795514334407 1108 3 -0.14136909104958306 1108 4 -0.10669950016468022 1108 5 -0.6808145193602329 1108 6 -0.7162011116681484 1108 7 -0.36097475469009765 1108 8 -0.3000936329391948 1108 9 -0.02320593001571325 1108 10-11 -0.37441406815101885 1108 12-13 -0.14900347279056803 1108 14-15 0.13450497877358458 1108 16-17 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-1.1025924674778054 2316 64-65 -0.961896483708852 2316 66-67 -0.8340180235701666 2316 68-69 -1.2755230973774516 2316 70-71 -1.0825450954317617 2316 72-73 -0.5767022321802706 2316 74-75 -0.8116942615172889 2316 76-77 -0.9387585157295071 2316 78-79 -1.231533323178951 2316 80-81 -0.8300215839373699 2316 82-83 -0.8642415156872723 2316 84-85 -0.7795164851383483 2316 86-87 -0.40971440317522223 2316 88-89 -1.1265300705814845 2316 90-91 -0.5937217594671971 2316 92-93 -0.3292012764900143 2316 94-95 -0.46587156298793886 2316 96-97 -0.37388900308749484 2316 98-99 -0.8147418431165292 2316 100-101 -0.9126353616540399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 37.0 7 992.0 8 2051.0 9 1571.0 10 1474.0 11 1654.0 12 1852.0 13 1657.0 14 1507.0 15 1434.0 16 1659.0 17 2008.0 18 2268.0 19 2767.0 20 3541.0 21 3864.0 22 3062.0 23 2401.0 24 1968.0 25 1591.0 26 1527.0 27 1568.0 28 1681.0 29 1613.0 30 1702.0 31 1908.0 32 2379.0 33 2978.0 34 3793.0 35 6140.0 36 8937.0 37 19966.0 38 42802.0 39 15303.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.27878516134988 25.64915797386224 15.498687837107175 18.5733690276807 2 14.963932004905775 25.23440899919556 41.15773648604096 18.643922509857706 3 15.750570362262458 33.13705838135805 34.401086655501196 16.711284600878294 4 11.144812670614144 22.7571245829432 41.933824789988 24.164237956454652 5 9.86034366798982 42.086800564427854 35.58533015073389 12.467525616848436 6 21.680359756821268 43.39302905220958 20.45853169631671 14.468079494652441 7 20.316765353624604 39.32466470611507 25.852905880336017 14.505664059924303 8 23.18835801606246 35.72050271004497 23.166598530905063 17.92454074298751 9 26.5914096190112 18.020809980350524 23.832570652388927 31.555209748249354 10-11 20.52018357092933 30.631420696567275 30.74879004074958 18.099605691753816 12-13 22.96812565113611 31.08045734481531 29.59949359743634 16.351923406612244 14-15 21.330559548457714 31.38212293449736 29.26815598253966 18.01916153450527 16-17 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34.548457714067176 17.06833797096098 42-43 16.46632554827309 31.87368948555302 33.51751968244339 18.1424652837305 44-45 17.04097376992971 31.68972292922233 33.54785108599612 17.721452214851837 46-47 17.36934418230492 31.631367946300227 32.862097614369176 18.137190257025676 48-49 16.450500468158623 32.001608883144975 32.76385024199185 18.78404040670456 50-51 16.58699178414591 31.30365691226312 32.19777393873057 19.91157736486041 52-53 16.537538408788194 31.82258766435005 32.40745625024727 19.23241767661449 54-55 17.346925318809426 31.35278059845178 31.20277202653338 20.09752205620541 56-57 17.31560484774954 31.67587598412217 30.799562172783503 20.20895699534479 58-59 16.5850136491316 30.517677933244535 31.528175236387135 21.36913318123673 60-61 17.918936027113638 30.291511163275263 31.372232259425815 20.417320550185288 62-63 17.03602843239394 30.014901950441125 32.32008862044864 20.628980996716294 64-65 17.160321249126323 29.94171095491171 31.72631842698704 21.17164936897493 66-67 17.396049004998087 30.49295124556568 30.804507510319272 21.306492239116963 68-69 17.124055440530668 29.732358332564058 31.67521660578407 21.46836962112121 70-71 17.08185522689209 29.351897031478725 32.59109311740891 20.975154624220284 72-73 17.374289519840694 28.915058882485596 31.91753814503686 21.793113452636852 74-75 17.05185351250841 29.18639306861491 31.321130438222845 22.440622980653842 76-77 17.010312677207928 29.324203141278403 31.977211884635164 21.688272296878502 78-79 17.40791781508394 28.718234448561898 31.636972662174102 22.236875074180062 80-81 17.664415988605942 28.538553851428873 31.49223911696053 22.304791043004656 82-83 17.55693731949518 29.14683036832874 31.255851982750666 22.040380329425417 84-85 17.354837858866663 28.305793298078573 30.99144126917143 23.347927573883343 86-87 17.21966529955558 28.696804652573555 31.40223397380949 22.681296074061375 88-89 18.11477139353018 28.01995278851099 31.06166506217938 22.803610755779452 90-91 18.304672354903797 27.464426538659353 31.254203536905408 22.976697569531446 92-93 18.638647483152884 27.446623323530577 30.936383177939835 22.9783460153767 94-95 18.423360455762307 26.43513695288082 31.178045338854528 23.96345725250234 96-97 19.391657545266323 25.980495588758917 30.651861425048466 23.975985440926294 98-99 19.012844690026242 26.44535731712142 30.535810837542364 24.005987155309974 100-101 19.27923353861979 26.247214126521516 31.10815123501563 23.365401099843066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 570.0 1 358.0 2 141.5 3 125.0 4 89.5 5 86.5 6 149.5 7 251.0 8 357.0 9 424.0 10 364.5 11 264.5 12 247.0 13 242.0 14 227.5 15 217.5 16 235.5 17 249.0 18 247.5 19 293.0 20 354.5 21 396.5 22 474.0 23 571.5 24 689.5 25 884.5 26 1279.5 27 1885.0 28 2360.0 29 2934.0 30 3926.5 31 4737.5 32 5802.0 33 7165.0 34 8513.5 35 9536.0 36 10413.0 37 10650.5 38 9231.0 39 7738.5 40 6661.0 41 5674.5 42 4884.5 43 4529.5 44 4452.0 45 4190.0 46 3606.0 47 2919.0 48 2581.0 49 2422.0 50 2285.0 51 2078.5 52 1668.0 53 1212.5 54 903.0 55 736.5 56 700.5 57 659.5 58 610.0 59 561.5 60 476.0 61 422.5 62 370.5 63 317.5 64 282.5 65 259.5 66 228.5 67 195.0 68 182.0 69 172.0 70 145.0 71 118.5 72 102.0 73 95.5 74 88.0 75 78.0 76 70.5 77 59.0 78 47.0 79 42.0 80 36.5 81 23.5 82 20.5 83 22.5 84 18.5 85 12.0 86 6.5 87 6.5 88 7.5 89 7.0 90 5.0 91 3.0 92 2.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.582376135779185 #Duplication Level Percentage of deduplicated Percentage of total 1 81.29330986473843 33.80368988118002 2 6.629878058449487 5.51372166321592 3 2.966874395445824 3.701090611771222 4 1.7997875140732282 2.9935776549868782 5 1.2035583464154893 2.5023407931002652 6 0.8991008991008992 2.24320510622585 7 0.737357880215023 2.1462764905247336 8 0.5312148169291027 1.7671339461156021 9 0.42655756941471223 1.5963549565469675 >10 3.1095888238745384 25.176383705442507 >50 0.282257425114568 8.041778211502196 >100 0.11258582687154117 7.191180155349536 >500 0.0047571476142904716 1.3332629996439356 >1k 0.0031714317428603147 1.990003824394361 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 1.0530272059502301 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1421 0.9369766184441309 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 780 0.5143151037202126 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 680 0.44837726990992893 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.3705706260137942 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 412 0.2716638752983687 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 366 0.2413324717456382 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 346 0.22814490498358148 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 273 0.1800102863020744 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 244 0.16088831449709215 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 223 0.14704136939693258 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 222 0.14638199105882974 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.14308509936831554 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 212 0.13978820767780137 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 208 0.13715069432539004 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 208 0.13715069432539004 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 205 0.1351725593110815 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 199 0.13121628928246448 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.1259412625776418 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 186 0.12264437088712761 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 182 0.12000685753471627 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 176 0.11605058750609924 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 170 0.11209431747748223 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 168 0.11077556080127655 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 163 0.10747866911076237 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 162 0.10681929077265954 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 160 0.10550053409645387 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 152 0.10022550739163118 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 152 0.10022550739163118 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.89067507154255E-4 0.0 14-15 0.0 0.0 0.0 0.00197813501430851 0.0 16-17 0.0 0.0 0.0 0.002967202521462765 0.0 18-19 0.0 0.0 0.0 0.0036265808595656018 0.0 20-21 0.0 0.0 0.0 0.005604715873874112 0.0 22-23 0.0 0.0 0.0 0.018462593466879427 0.0 24-25 0.0 0.0 0.0 0.04285959197668438 0.0 26-27 0.0 0.0 0.0 0.08341135977000884 0.0 28-29 0.0 0.0 0.0 0.17176805707578896 0.0 30-31 0.0 0.0 0.0 0.29342336045576234 0.0 32-33 0.0 0.0 0.0 0.5060728744939271 0.0 34-35 0.0 0.0 0.0 0.8634559337456646 0.0 36-37 0.0 0.0 0.0 1.4008492792994764 0.0 38-39 0.0 0.0 0.0 1.9151643830196892 0.0 40-41 0.0 0.0 0.0 2.3609041395772064 0.0 42-43 0.0 0.0 0.0 2.760817101636577 0.0 44-45 0.0 0.0 0.0 3.2276569650133853 0.0 46-47 0.0 0.0 0.0 3.771973783117277 0.0 48-49 0.0 0.0 0.0 4.240462092339342 0.0 50-51 0.0 0.0 0.0 4.678948687177729 0.0 52-53 0.0 0.0 0.0 5.09073045932295 0.0 54-55 0.0 0.0 0.0 5.579329807857152 0.0 56-57 0.0 0.0 0.0 6.146724867794643 0.0 58-59 0.0 0.0 0.0 6.700932360970078 0.0 60-61 0.0 0.0 0.0 7.224808450592781 0.0 62-63 0.0 0.0 0.0 7.690988935631487 0.0 64-65 0.0 0.0 0.0 8.231679172875811 0.0 66-67 0.0 0.0 0.0 8.861385485764021 0.0 68-69 0.0 0.0 0.0 9.52340133721927 0.0 70-71 0.0 0.0 0.0 10.164317081855227 0.0 72-73 0.0 0.0 0.0 10.774571733769402 0.0 74-75 0.0 0.0 0.0 11.42043281594113 0.0 76-77 0.0 0.0 0.0 12.146078677023302 0.0 78-79 0.0 0.0 0.0 12.916562265096466 0.0 80-81 0.0 0.0 0.0 13.655066003771644 0.0 82-83 0.0 0.0 0.0 14.326313151960331 0.0 84-85 0.0 0.0 0.0 14.963272626567672 0.0 86-87 0.0 0.0 0.0 15.707051391947672 0.0 88-89 0.0 0.0 0.0 16.458413008215857 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAT 35 2.7802135E-4 54.285717 1 CGCAGAA 70 4.2854426E-6 40.714287 9 GTATATG 95 1.9093932E-8 40.0 1 GGTATCA 1310 0.0 39.885494 1 CGAAAAC 30 8.276316E-5 39.583336 90-91 CATGGGT 130 2.0190782E-10 36.538464 4 TATATGG 105 4.6120476E-8 36.190475 2 ATGGGGT 70 2.0449678E-4 33.928574 5 CATGGGG 310 0.0 33.70968 4 ATGGGTA 85 1.6229165E-5 33.52941 5 TACCATA 60 0.0039402395 31.666668 7 ATATGGG 105 1.8873179E-6 31.666666 3 GTATCAA 1935 0.0 31.666666 1 AACGCAG 1960 0.0 30.778063 6 TCGCCGG 40 4.4859303E-4 29.687498 94-95 TGTTATG 80 4.4754703E-4 29.687498 2 CGCAGAG 2045 0.0 29.498775 8 ATGGGGG 115 3.835554E-6 28.913044 5 GCAGAGT 2045 0.0 28.801954 9 ACGCAGA 2110 0.0 28.590048 7 >>END_MODULE