##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031559_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 151658 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.571549143467536 34.0 31.0 34.0 31.0 34.0 2 32.726694272639755 34.0 31.0 34.0 31.0 34.0 3 32.77176937583246 34.0 31.0 34.0 31.0 34.0 4 36.2445766131691 37.0 35.0 37.0 35.0 37.0 5 36.18372918012897 37.0 37.0 37.0 35.0 37.0 6 36.22150496511889 37.0 37.0 37.0 35.0 37.0 7 36.2370003560643 37.0 37.0 37.0 35.0 37.0 8 36.26906592464624 37.0 37.0 37.0 35.0 37.0 9 38.064401482282506 39.0 39.0 39.0 35.0 39.0 10-11 38.02150562449722 39.0 39.0 39.0 35.0 39.0 12-13 38.00394308246186 39.0 39.0 39.0 35.0 39.0 14-15 39.409167337034646 41.0 39.0 41.0 36.0 41.0 16-17 39.30763626053357 41.0 39.0 41.0 36.0 41.0 18-19 39.40224716137625 41.0 39.0 41.0 36.0 41.0 20-21 39.468877342441544 41.0 39.0 41.0 36.0 41.0 22-23 39.43189610834905 41.0 39.0 41.0 36.0 41.0 24-25 39.34987603687244 41.0 39.0 41.0 35.0 41.0 26-27 39.178292605731315 41.0 39.0 41.0 35.0 41.0 28-29 39.00734217779477 41.0 39.0 41.0 35.0 41.0 30-31 38.66460061454062 40.0 38.0 41.0 35.0 41.0 32-33 37.47933508288386 39.0 35.5 41.0 33.0 41.0 34-35 37.469833441031795 40.0 36.0 41.0 33.0 41.0 36-37 37.53236888261747 40.0 36.0 41.0 33.0 41.0 38-39 37.01510306083425 40.0 35.0 41.0 31.5 41.0 40-41 36.9946458478946 40.0 35.0 41.0 31.0 41.0 42-43 36.664857772092475 40.0 35.0 41.0 30.5 41.0 44-45 36.272019939600945 40.0 35.0 41.0 28.0 41.0 46-47 35.75465191417531 40.0 35.0 41.0 24.0 41.0 48-49 35.3768248295507 40.0 35.0 41.0 20.0 41.0 50-51 34.88847604478498 40.0 35.0 41.0 15.0 41.0 52-53 35.042322198631126 40.0 35.0 41.0 15.0 41.0 54-55 34.935245750306606 40.0 35.0 41.0 13.0 41.0 56-57 34.58723905102269 40.0 35.0 41.0 10.0 41.0 58-59 34.40580450751032 39.5 34.5 41.0 10.0 41.0 60-61 34.00398594205383 39.0 33.5 41.0 10.0 41.0 62-63 33.55826596684646 39.0 33.5 41.0 10.0 41.0 64-65 33.10639069485289 39.0 33.0 41.0 8.0 41.0 66-67 32.41592926189189 38.0 31.0 41.0 7.5 41.0 68-69 31.448799272046315 37.0 27.5 40.0 7.0 41.0 70-71 30.69823550356724 37.0 24.0 40.0 7.0 41.0 72-73 30.13881232773741 36.0 20.5 39.0 7.0 41.0 74-75 29.71230663730235 36.0 20.0 39.0 7.0 41.0 76-77 28.50206055730657 34.5 19.5 36.5 7.0 39.0 78-79 29.08777644436825 35.0 20.0 37.0 7.0 39.0 80-81 28.924286882327344 35.0 20.0 37.0 7.0 39.0 82-83 28.66216421158132 35.0 20.0 36.0 7.0 39.0 84-85 28.3669539358293 35.0 20.0 36.0 7.0 37.5 86-87 28.116406651808674 35.0 19.5 36.0 7.0 37.0 88-89 27.870442047237866 35.0 19.0 35.0 7.0 37.0 90-91 27.70428859671102 35.0 19.0 35.0 7.0 36.0 92-93 27.560877105065344 35.0 19.0 35.0 7.0 36.0 94-95 27.427537617534192 35.0 19.0 35.0 7.0 36.0 96-97 27.320352371783883 35.0 18.5 35.0 7.0 35.5 98-99 27.228355906051775 35.0 18.0 35.0 7.0 35.0 100-101 26.349790317688484 33.5 16.5 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22569680254638058 1101 2 0.1851270318944458 1101 3 0.34464178912045895 1101 4 0.210160053236514 1101 5 0.2917292074257034 1101 6 0.33107800986073244 1101 7 0.19449159178120112 1101 8 0.3440673606776059 1101 9 0.21302988136262968 1101 10-11 0.30686672972280604 1101 12-13 0.20708382211605425 1101 14-15 0.3180245073235639 1101 16-17 0.053937256732695005 1101 18-19 0.3189290227803241 1101 20-21 0.5062423620949019 1101 22-23 0.5364307264326058 1101 24-25 0.5298163559017652 1101 26-27 0.5244063725682722 1101 28-29 0.6671243945632952 1101 30-31 0.497987793852829 1101 32-33 0.4286606570281464 1101 34-35 0.1735795463010561 1101 36-37 0.21728952898521214 1101 38-39 0.3884566411398751 1101 40-41 -0.14133545750881638 1101 42-43 -0.2603528629370331 1101 44-45 -0.48700797872366053 1101 46-47 0.14399793314903064 1101 48-49 0.3377945516952394 1101 50-51 0.23964638561782436 1101 52-53 0.2821210570274246 1101 54-55 0.09958714322406337 1101 56-57 0.06922501453376384 1101 58-59 0.19806867111110904 1101 60-61 0.10240712482979575 1101 62-63 -0.5476638607459847 1101 64-65 0.08976137879128743 1101 66-67 -0.14147969187995812 1101 68-69 0.23988323761345498 1101 70-71 0.1054802892168567 1101 72-73 0.3389310515946029 1101 74-75 0.423171290850199 1101 76-77 0.444652505996725 1101 78-79 0.8969838881407064 1101 80-81 0.7615651382873878 1101 82-83 0.830282993368904 1101 84-85 0.4150426717914577 1101 86-87 0.43660920864536834 1101 88-89 0.8607561494956215 1101 90-91 0.5270913015070562 1101 92-93 0.21698433434799114 1101 94-95 0.36502521131707155 1101 96-97 0.12188892063299406 1101 98-99 -0.1247022490615386 1101 100-101 -0.08644985366563773 1102 1 0.2554718762926882 1102 2 -0.15631839583416252 1102 3 -0.14632428757570892 1102 4 0.06414235412147207 1102 5 -0.11820810820852046 1102 6 0.010650281247166049 1102 7 -0.1045497061539038 1102 8 0.03608432232952907 1102 9 -0.17026657881436336 1102 10-11 0.01828818989979908 1102 12-13 -0.22718977670400164 1102 14-15 -0.6355153156852893 1102 16-17 -0.2968647344177455 1102 18-19 0.25762740036145004 1102 20-21 0.43987068067897184 1102 22-23 0.19599931050340302 1102 24-25 0.30968361253893306 1102 26-27 -0.024127919467126446 1102 28-29 0.06134452730665885 1102 30-31 0.1303624251212696 1102 32-33 0.3886532823968807 1102 34-35 0.12200321886742671 1102 36-37 0.09247389476693257 1102 38-39 0.3314876145912038 1102 40-41 0.46310775783042146 1102 42-43 0.43724116361163823 1102 44-45 0.15624423366571705 1102 46-47 -0.2145935122787037 1102 48-49 -1.2552179851779144 1102 50-51 -1.310639381343826 1102 52-53 -1.432250255656946 1102 54-55 -1.258036381849685 1102 56-57 -1.12551134239839 1102 58-59 -0.9714095737266533 1102 60-61 -1.8107105005389386 1102 62-63 -2.1268028725453902 1102 64-65 -1.7638060990848174 1102 66-67 -1.9110224647413183 1102 68-69 -1.4939386650414122 1102 70-71 -1.5115458907241468 1102 72-73 -1.5379586976679356 1102 74-75 -1.588351570506731 1102 76-77 -1.023083482203866 1102 78-79 -1.0184383094994125 1102 80-81 -1.497514876461878 1102 82-83 -1.3781886143007114 1102 84-85 -0.7725034196539688 1102 86-87 -0.9473325317676142 1102 88-89 -1.2710653844276827 1102 90-91 -1.3238950554250977 1102 92-93 -0.7493228190443411 1102 94-95 -0.4289921190664039 1102 96-97 -0.8624860793670059 1102 98-99 -0.5774124260526889 1102 100-101 -1.059255900863274 1103 1 0.27831432391389654 1103 2 0.3881184848859007 1103 3 0.37829563527430565 1103 4 0.21443355751002002 1103 5 0.2722313441778397 1103 6 0.27659083037355714 1103 7 0.1806027028923083 1103 8 0.25565924101948667 1103 9 0.2253162061489533 1103 10-11 -0.014046731815653857 1103 12-13 -0.015405494123257313 1103 14-15 -0.08582164652258939 1103 16-17 0.22314131656175107 1103 18-19 0.13543543303674 1103 20-21 0.10025945611199205 1103 22-23 0.16169782044969594 1103 24-25 0.14199584308125424 1103 26-27 0.4267835093204084 1103 28-29 0.4127173432812441 1103 30-31 0.5118766827417218 1103 32-33 0.6428700587375502 1103 34-35 0.586907537754044 1103 36-37 0.8438920930877813 1103 38-39 1.2287344189176537 1103 40-41 1.2291239442005946 1103 42-43 0.860507179798013 1103 44-45 0.8671586879430109 1103 46-47 0.5676090442601307 1103 48-49 0.6948992525499378 1103 50-51 1.1748760864725298 1103 52-53 1.5645729801043444 1103 54-55 1.2885561603180733 1103 56-57 1.0020508692346155 1103 58-59 1.4295056369230679 1103 60-61 1.6102863983340612 1103 62-63 1.3109098572027378 1103 64-65 0.6003115924665039 1103 66-67 0.9087339833337111 1103 68-69 0.9056150752202967 1103 70-71 0.88793221229378 1103 72-73 0.7781671627057101 1103 74-75 1.4330537694826795 1103 76-77 1.4109986598428783 1103 78-79 1.3025661530979704 1103 80-81 1.7098824459796944 1103 82-83 1.7964956002065087 1103 84-85 1.899684765808555 1103 86-87 1.7520472428334024 1103 88-89 2.0290253802648515 1103 90-91 1.5515304040711548 1103 92-93 1.82555805229671 1103 94-95 1.7585882455051056 1103 96-97 1.556317339436415 1103 98-99 1.2874238193145295 1103 100-101 1.0890309155651359 1104 1 0.5250627445753651 1104 2 0.39889514783647684 1104 3 0.3437359920190062 1104 4 0.24639193729448294 1104 5 0.07026181612135218 1104 6 0.36187511131001315 1104 7 0.3303611569985918 1104 8 0.32459272299644937 1104 9 0.4874864031017623 1104 10-11 0.2409699905923688 1104 12-13 0.489239619217507 1104 14-15 0.6042563913815329 1104 16-17 0.47490645963124223 1104 18-19 0.4063384430701831 1104 20-21 0.2634887389064957 1104 22-23 0.2488401467224648 1104 24-25 0.31061345735103885 1104 26-27 0.22119079285813115 1104 28-29 0.3987820032589511 1104 30-31 0.31139359095428176 1104 32-33 0.5600012367382945 1104 34-35 0.7983530970256965 1104 36-37 0.4029779347823208 1104 38-39 0.6248696846181332 1104 40-41 0.6317623685781442 1104 42-43 0.2489315573528259 1104 44-45 0.3454195575082295 1104 46-47 0.1485269186562732 1104 48-49 0.11723295749234097 1104 50-51 0.16605037112507404 1104 52-53 0.6016409845636517 1104 54-55 0.7134911287312988 1104 56-57 0.5930021884468033 1104 58-59 0.5687661348792261 1104 60-61 0.6085212552645771 1104 62-63 0.6002075160656162 1104 64-65 0.6402595671970843 1104 66-67 0.548737699424386 1104 68-69 0.33612417964243946 1104 70-71 0.5481886225501889 1104 72-73 0.840969095072861 1104 74-75 0.7044212908502026 1104 76-77 0.6049785929532447 1104 78-79 0.3419567142276634 1104 80-81 0.6521901382873843 1104 82-83 0.4360348049631071 1104 84-85 0.4071169471537779 1104 86-87 0.8410476144424663 1104 88-89 0.457676439350692 1104 90-91 0.7750532580287945 1104 92-93 0.8105078850726279 1104 94-95 0.228023399722872 1104 96-97 0.3693979786040096 1104 98-99 0.892960794416723 1104 100-101 0.7908146390879871 1105 1 0.008798654398226802 1105 2 0.0017936985611157752 1105 3 0.19140104837971705 1105 4 -0.10465476157830267 1105 5 -0.1978541259076323 1105 6 0.004226158008883374 1105 7 0.28430640659601636 1105 8 0.034576619936864006 1105 9 0.27830765914040967 1105 10-11 0.2771213593524351 1105 12-13 0.04527826656050138 1105 14-15 0.13098747028652724 1105 16-17 3.492937697302523E-4 1105 18-19 0.273327170928475 1105 20-21 0.3766127324652686 1105 22-23 -0.26194890319703035 1105 24-25 -0.15999845891305142 1105 26-27 0.10484618738308882 1105 28-29 0.2454808760447733 1105 30-31 0.3452100160750575 1105 32-33 0.4930125088799997 1105 34-35 0.30286195370846514 1105 36-37 0.3057154549111445 1105 38-39 0.8028084929917227 1105 40-41 0.35334046841711597 1105 42-43 0.3992536185444422 1105 44-45 0.5560475768318938 1105 46-47 0.6676090442601392 1105 48-49 0.7125630702137542 1105 50-51 0.8666139782104167 1105 52-53 0.8970516125829775 1105 54-55 0.7159065876685062 1105 56-57 0.6880907552745015 1105 58-59 0.7081381155555562 1105 60-61 0.4108561989038648 1105 62-63 0.19666484295772335 1105 64-65 0.19034008249499124 1105 66-67 -0.0220352474355181 1105 68-69 -0.03199176238654644 1105 70-71 0.016707141068707188 1105 72-73 -0.04035135581280613 1105 74-75 0.6284953649242766 1105 76-77 0.9682636171078336 1105 78-79 0.5413125918444095 1105 80-81 0.1133012493984964 1105 82-83 -0.0177494140385015 1105 84-85 0.39826026438405293 1105 86-87 0.4289703197564769 1105 88-89 -0.09804014680067397 1105 90-91 0.20452185706260906 1105 92-93 0.3224241491628028 1105 94-95 0.17625206316892772 1105 96-97 0.31158799470707166 1105 98-99 0.3190477509384628 1105 100-101 -0.15057022403600584 1106 1 0.2046353670232648 1106 2 0.07138007325697515 1106 3 0.0893194046922332 1106 4 0.06052501674016497 1106 5 0.1525108375960187 1106 6 0.17627265706268247 1106 7 0.057326141659544305 1106 8 0.08091346773356634 1106 9 -0.20165989965926912 1106 10-11 0.14092238665710966 1106 12-13 0.04049318951265235 1106 14-15 0.1264186679075081 1106 16-17 -0.19800161188044285 1106 18-19 0.03296491085819042 1106 20-21 0.2544175445766541 1106 22-23 0.034834011104138085 1106 24-25 -0.020366125850060257 1106 26-27 0.04185619008652708 1106 28-29 -0.0505991455826873 1106 30-31 0.143669059059647 1106 32-33 0.43945749401111556 1106 34-35 0.19399469228645927 1106 36-37 -0.33456570215833636 1106 38-39 -0.23882438075794 1106 40-41 -0.3599333893822916 1106 42-43 -0.273468738849445 1106 44-45 -0.05498243127841107 1106 46-47 -0.5495847919118049 1106 48-49 -0.28819389112714333 1106 50-51 -0.0746404148687887 1106 52-53 -0.24414871182902687 1106 54-55 0.41501445465957687 1106 56-57 0.4770337128305968 1106 58-59 0.39313406040551513 1106 60-61 0.17863127324828554 1106 62-63 0.13797330470413982 1106 64-65 0.09466557587158064 1106 66-67 -0.03898577460502395 1106 68-69 -0.2933056309996829 1106 70-71 -0.45363619496805896 1106 72-73 -1.0458393255343523 1106 74-75 -1.1100555947945665 1106 76-77 -1.0542830171176334 1106 78-79 -0.8808825960442093 1106 80-81 -0.8690993994255081 1106 82-83 -0.705719135584868 1106 84-85 -0.48508658611607913 1106 86-87 -1.1921164604543897 1106 88-89 -1.0143959186308997 1106 90-91 -1.349087226473479 1106 92-93 -1.011612076844223 1106 94-95 -0.39847083491163815 1106 96-97 -1.252997028272116 1106 98-99 -1.1203683074556992 1106 100-101 -1.1753336736169722 1107 1 0.5361962506699953 1107 2 0.45389082880394227 1107 3 0.3047687206877825 1107 4 0.2995640267464452 1107 5 0.21936441713872767 1107 6 0.4913980981609569 1107 7 0.3851648809644246 1107 8 0.4702400979844512 1107 9 0.4766888217599785 1107 10-11 0.2852400740936716 1107 12-13 0.38140777355094 1107 14-15 0.45213046758846787 1107 16-17 0.630199673951239 1107 18-19 0.47241964088187416 1107 20-21 0.3804145475253691 1107 22-23 0.41536284563790815 1107 24-25 0.5455448327229604 1107 26-27 0.5979783924358202 1107 28-29 0.4772444276758776 1107 30-31 0.7068730035658604 1107 32-33 1.3460171691694285 1107 34-35 1.2577062019301977 1107 36-37 1.0888403016121444 1107 38-39 0.9211553166365647 1107 40-41 0.8550083614757398 1107 42-43 0.6786992893808517 1107 44-45 0.5680416901505154 1107 46-47 0.9421491472770569 1107 48-49 1.425611880613559 1107 50-51 1.5579065953308557 1107 52-53 1.7033062115528068 1107 54-55 1.5683716354977904 1107 56-57 1.7919416260127932 1107 58-59 1.683340470228103 1107 60-61 2.119342831231556 1107 62-63 2.2209964593423237 1107 64-65 2.489285385413801 1107 66-67 2.394668210989799 1107 68-69 2.117677111785639 1107 70-71 2.4505065033448936 1107 72-73 2.300610135479811 1107 74-75 2.410271180474922 1107 76-77 1.8112363912064353 1107 78-79 1.9040548042554946 1107 80-81 1.9960091228348489 1107 82-83 2.0781789646713342 1107 84-85 2.301252660753935 1107 86-87 2.0401550143848795 1107 88-89 2.2772848470673637 1107 90-91 2.5041539394761507 1107 92-93 2.36809498556212 1107 94-95 2.2994553989550433 1107 96-97 2.197116569639615 1107 98-99 2.1395775522629634 1107 100-101 2.086908314104786 1108 1 0.22880827440784657 1108 2 0.31391491068232824 1108 3 0.18582144280011192 1108 4 0.05431589739235676 1108 5 0.0878601598066524 1108 6 0.3153853691680908 1108 7 0.184210206499813 1108 8 0.27358575894600534 1108 9 0.38754286837561835 1108 10-11 0.15528393751501568 1108 12-13 0.07464334592558686 1108 14-15 0.09724528654434295 1108 16-17 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-0.8121563700752574 2316 80-81 -1.102504697393364 2316 82-83 -1.4464922413728765 2316 84-85 -1.4478534549691027 2316 86-87 -1.0779154392419592 2316 88-89 -1.1618377472179944 2316 90-91 -1.0275595200891878 2316 92-93 -0.7327662525064689 2316 94-95 -0.7615152816406727 2316 96-97 -0.7591996943904782 2316 98-99 -0.8091212631460429 2316 100-101 -0.6942843607078863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 13.0 14 68.0 15 212.0 16 514.0 17 1081.0 18 1542.0 19 2135.0 20 2908.0 21 4119.0 22 3971.0 23 3021.0 24 2669.0 25 2883.0 26 3511.0 27 4377.0 28 5402.0 29 4476.0 30 2089.0 31 1775.0 32 1953.0 33 2519.0 34 3227.0 35 5077.0 36 7571.0 37 19211.0 38 47070.0 39 18264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.54497675892668 26.1323420663427 15.068402704415803 23.25427847031481 2 13.0174471508262 23.239130148096372 45.86503844175711 17.878384259320313 3 13.310211132943861 33.425206715109 38.8182621424521 14.446320009495048 4 9.02359255693732 26.68767885637421 38.988381753682624 25.300346833005843 5 7.510978649329412 41.7722770971528 39.5172031808411 11.19954107267668 6 18.07026335570824 45.98108902926321 20.085323556950506 15.863324058078042 7 16.978992206148042 38.84595603265242 24.05214363897717 20.12290812222237 8 20.605573065713646 38.450988408128815 24.505795935591923 16.437642590565616 9 23.073626185232563 16.44819264397526 22.116868216645347 38.36131295414683 10-11 18.68480396682008 30.65252080338657 32.08436086457688 18.578314365216475 12-13 22.017631776760872 28.617679252001217 30.16952617072624 19.195162800511678 14-15 19.911907054029463 29.763678803623943 27.02626963299002 23.29814450935658 16-17 17.93838768808767 34.8890266256973 27.933244537050467 19.239341149164567 18-19 17.851020058289045 29.722137968323466 32.86836170858115 19.55848026480634 20-21 17.137572696461774 28.73867517704308 32.75989397196323 21.363858154531908 22-23 17.044930039958327 32.84594284508565 31.921494415065478 18.187632699890543 24-25 18.769863772435347 34.24448430020177 31.919186590882116 15.066465336480766 26-27 18.437866779200572 33.541257302615094 33.72291603476242 14.29795988342191 28-29 16.1432301626027 29.699389415658917 36.657149639320046 17.500230782418335 30-31 17.08745994276596 34.923314299278644 32.75066267522979 15.238563082725607 32-33 21.26165451212597 30.592187685450156 34.04568173126377 14.10047607116011 34-35 17.388466154109906 30.35250365954978 34.455815057563726 17.80321512877659 36-37 15.671444961690117 34.516477864669184 33.036503184797375 16.775573988843316 38-39 15.058552796423532 30.015891017948277 35.844465837608304 19.081090348019885 40-41 14.431483996887733 29.504213427580478 37.75270674807791 18.31159582745388 42-43 17.59952524603135 28.470072367011195 36.45912665051184 17.47127573644561 44-45 15.001846259346689 30.99968349839771 36.28723839164436 17.711231850611245 46-47 18.41284494411658 28.029079159935378 33.40212983416307 20.15594606178497 48-49 17.24153847168281 28.013214029026024 33.07925120502186 21.665996294269306 50-51 14.874635280154294 27.638593541367882 35.537642384979314 21.949128793498506 52-53 15.005802529375305 29.007042160650936 38.348784765722876 17.638370544250883 54-55 15.289005525590474 28.8593414129159 33.90820134776932 21.9434517137243 56-57 18.334014690949374 30.837806116393462 29.80488994975537 21.02328924290179 58-59 14.090255706919516 32.12095636234159 34.78220733492463 19.006580595814267 60-61 17.70733395974482 35.53533455318728 28.639203468341496 18.118128018726406 62-63 14.125202758838967 38.360653575808726 29.088805074575692 18.425338590776615 64-65 14.066518086747815 38.07250524205779 28.60119479354864 19.259781877645757 66-67 14.657321077687957 38.11635390154163 27.87818644581888 19.348138574951534 68-69 14.69972469955985 36.78640312556667 28.651027843260085 19.862844331613392 70-71 14.784910786110855 35.71291985915679 29.736644291761728 19.76552506297063 72-73 15.632211950573 33.93062021126482 29.319917182080736 21.117250656081445 74-75 15.800353426789224 33.62433897321605 28.68955149085442 21.885756109140303 76-77 15.793759643408196 33.5893919212966 29.560919964657323 21.055928470637884 78-79 16.021245170053675 32.58581809070408 29.19133840615068 22.20159833309156 80-81 16.081248598821034 32.43185324875707 29.01891097073679 22.467987181685107 82-83 16.395112687757983 32.59504938743753 28.810877105065348 22.19896081973915 84-85 16.318954489707103 31.804784449221273 28.504266177847526 23.371994883224094 86-87 16.038389006844348 32.416687546980704 28.852088251196772 22.692835194978176 88-89 16.85535876775376 31.618839757876277 28.44294399240396 23.082857481966002 90-91 16.72183465428794 31.151999894499465 28.57053370082686 23.555631750385736 92-93 17.091086523625528 31.11606377507286 28.234250748394413 23.5585989529072 94-95 17.24999670310831 30.022155112160252 28.578116551715045 24.14973163301639 96-97 18.001688008545543 29.69576283479935 28.15644410449828 24.146105052156827 98-99 17.28623003247556 30.339097608018335 27.98008605199387 24.394586307512238 100-101 16.827171931305283 30.766897561265093 28.63016092287505 23.775769584554567 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 597.0 1 370.5 2 143.5 3 126.0 4 99.5 5 94.5 6 156.0 7 251.0 8 353.5 9 411.5 10 323.0 11 220.0 12 219.0 13 231.0 14 210.0 15 214.0 16 212.5 17 204.5 18 240.0 19 283.5 20 324.5 21 381.0 22 439.5 23 524.0 24 627.0 25 821.0 26 1193.5 27 1748.5 28 2244.0 29 2754.0 30 3589.0 31 4457.0 32 5696.0 33 7054.5 34 8458.5 35 9606.0 36 10571.0 37 11128.0 38 9875.0 39 8531.5 40 7706.0 41 6789.0 42 6062.5 43 5670.5 44 5363.0 45 5075.0 46 4369.5 47 3331.5 48 2781.5 49 2447.0 50 2295.5 51 1931.5 52 1209.0 53 604.5 54 350.5 55 260.5 56 194.0 57 143.5 58 110.5 59 88.0 60 63.0 61 39.5 62 24.5 63 16.5 64 14.0 65 15.5 66 7.5 67 1.0 68 2.0 69 2.0 70 1.5 71 1.0 72 0.5 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.133194424296773 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 3.296891690514183E-4 44-45 0.0 46-47 0.00197813501430851 48-49 6.593783381028366E-4 50-51 3.296891690514183E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 3.296891690514183E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0036265808595656013 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0036265808595656013 100-101 9.89067507154255E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 151658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.016326207651428 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18312975122973 19.942897835920295 2 10.164983878180236 5.695710084532303 3 4.58236249382193 3.851428872858669 4 2.7089364306055685 3.03577786862546 5 1.9040222175151216 2.6671853776259744 6 1.3933017957589022 2.3421118569412758 7 1.0332085951658077 2.026269632990017 8 0.8025606627597732 1.7987841063445384 9 0.6825295958954082 1.7209774624484038 >10 4.8553743321800935 26.168748104287275 >50 0.4730636164654381 8.945785913041185 >100 0.19534467744592715 9.075024067309341 >500 0.009414201322695286 1.9867069327038467 >1k 0.009414201322695286 5.466246422872516 >5k 0.0023535503306738215 5.276345461498899 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 8002 5.276345461498899 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 2922 1.9267035039364888 RNA PCR Primer, Index 46 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 1995 1.3154597845151592 RNA PCR Primer, Index 46 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1742 1.1486370649751416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 1.0754460694457266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.5789341808542906 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 778 0.5129963470440069 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 700 0.46156483667198567 RNA PCR Primer, Index 46 (96% over 26bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.43321156813356365 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 411 0.27100449696026585 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 386 0.25452003850769495 RNA PCR Primer, Index 46 (96% over 28bp) CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 366 0.2413324717456382 TruSeq Adapter, Index 4 (95% over 23bp) ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 322 0.21231982486911338 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 297 0.19583536641654248 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC 293 0.19319785306413112 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.1780321512877659 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 268 0.17671339461156021 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 263 0.17341650292104605 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 252 0.16616334120191484 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 250 0.16484458452570916 TruSeq Adapter, Index 18 (95% over 24bp) ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 249 0.16418520618760632 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 246 0.1622070711732978 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 237 0.1562726661303723 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 236 0.15561328779226943 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 232 0.1529757744398581 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 220 0.14506323438262406 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 217 0.14308509936831554 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 215 0.14176634269210986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.13781007266349285 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 194 0.1279193975919503 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 179 0.11802872252040775 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 172 0.11341307415368791 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 172 0.11341307415368791 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 171 0.11275369581558506 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 171 0.11275369581558506 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 171 0.11275369581558506 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 169 0.11143493913937938 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 169 0.11143493913937938 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 168 0.11077556080127655 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 165 0.10879742578696805 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 164 0.10813804744886522 No Hit CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 163 0.10747866911076237 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 158 0.10418177742024819 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 158 0.10418177742024819 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.10220364240593968 No Hit AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT 153 0.100884885729734 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.89067507154255E-4 0.0 14-15 0.0 0.0 0.0 0.00197813501430851 0.0 16-17 0.0 0.0 0.0 0.002967202521462765 0.0 18-19 0.0 0.0 0.0 0.0036265808595656018 0.0 20-21 0.0 0.0 0.0 0.005604715873874112 0.0 22-23 0.0 0.0 0.0 0.01813290429782801 0.0 24-25 0.0 0.0 0.0 0.043189281145735794 0.0 26-27 0.0 0.0 0.0 0.08242229226285458 0.0 28-29 0.0 0.0 0.0 0.17011961123053185 0.0 30-31 0.0 0.0 0.0 0.292434292948608 0.0 32-33 0.0 0.0 0.0 0.5050838069867729 0.0 34-35 0.0 0.0 0.0 0.8684012712814358 0.0 36-37 0.0 0.0 0.0 1.4117290218781733 0.0 38-39 0.0 0.0 0.0 1.937253557346134 0.0 40-41 0.0 0.0 0.0 2.385630827256063 0.0 42-43 0.0 0.0 0.0 2.7881813026678444 0.0 44-45 0.0 0.0 0.0 3.2556805443827557 0.0 46-47 0.0 0.0 0.0 3.8016458083319047 0.0 48-49 0.0 0.0 0.0 4.274420076751639 0.0 50-51 0.0 0.0 0.0 4.714884806604333 0.0 52-53 0.0 0.0 0.0 5.121721241213784 0.0 54-55 0.0 0.0 0.0 5.610979968086088 0.0 56-57 0.0 0.0 0.0 6.173429690487808 0.0 58-59 0.0 0.0 0.0 6.726318426987037 0.0 60-61 0.0 0.0 0.0 7.2478866924263805 0.0 62-63 0.0 0.0 0.0 7.713078109957932 0.0 64-65 0.0 0.0 0.0 8.251790212187949 0.0 66-67 0.0 0.0 0.0 8.87688087670944 0.0 68-69 0.0 0.0 0.0 9.538567038995634 0.0 70-71 0.0 0.0 0.0 10.180142161969695 0.0 72-73 0.0 0.0 0.0 10.785121787179047 0.0 74-75 0.0 0.0 0.0 11.42933442350552 0.0 76-77 0.0 0.0 0.0 12.155969352094845 0.0 78-79 0.0 0.0 0.0 12.934695169394296 0.0 80-81 0.0 0.0 0.0 13.680452069788604 0.0 82-83 0.0 0.0 0.0 14.3536773529916 0.0 84-85 0.0 0.0 0.0 14.986680557570324 0.0 86-87 0.0 0.0 0.0 15.73705310633135 0.0 88-89 0.0 0.0 0.0 16.49401943847341 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATGCCA 35 2.780404E-4 54.284966 9 CATTCCG 60 1.4838042E-6 47.49934 9 GGGATGC 40 5.3736754E-4 47.49934 7 TATGGGA 55 4.9432747E-5 43.181217 4 GTATAGT 70 4.247122E-6 40.767487 1 ATGGGTA 70 4.28585E-6 40.713722 5 ACTATAC 70 4.28585E-6 40.713722 3 CATGGGT 95 1.909575E-8 39.999447 4 ATGCCAC 30 8.276993E-5 39.582783 10-11 GGTATCA 1110 0.0 38.992325 1 CCTTTAG 50 0.0016010844 38.049652 1 GATGACC 25 0.0016140505 37.999474 22-23 TAGGCAT 50 0.0016115052 37.999474 5 AGGCTTA 75 6.8833087E-6 37.99947 6 CTATACA 80 1.0619022E-5 35.67155 1 GTACCCA 55 0.002556168 34.59059 1 GGATGCC 55 0.0025727754 34.544975 8 GTAGGGG 55 0.0025727754 34.544975 6 GGCATTC 55 0.0025727754 34.544975 7 AGGGGTG 55 0.0025727754 34.544975 8 >>END_MODULE