FastQCFastQC Report
Fri 10 Feb 2017
SRR2031558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179139
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36850.16910348536738593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.11715636313241147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.049.7264561
GTATCAA47800.041.338821
TCAACGC52500.036.5523034
CAACGCA53300.036.0927935
AACGCAG54350.035.3955086
ATCAACG54500.035.210933
TATCAAC58600.032.8284262
ACGCAGA62050.030.7735027
CGCAGAG63150.029.9365928
GTACATG54100.028.3594591
CCGTACG856.4091187E-427.9411149
ACATGGG52700.026.8595223
TACATGG55750.026.49772
GCAGAGT73000.025.3766579
CATGGGG31950.025.2738114
GAGTACT42750.021.55550812-13
CAGAGTA70050.021.393610-11
AGAGTAC65550.021.23183810-11
GTGTACG2351.1330667E-720.212721
AATACCG1951.03336115E-519.4871375
Produced by FastQC (version 0.11.5)