FastQCFastQC Report
Fri 10 Feb 2017
SRR2031558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179139
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44550.2044385420113173No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT40600.18631211684982002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.14785656169707392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.050.3203621
GTATCAA51350.044.55391
TCAACGC57400.039.3892634
CAACGCA57600.039.334965
AACGCAG58000.039.2274746
ATCAACG59600.037.8556023
TATCAAC63300.035.717912
ACGCAGA67150.033.599317
CGCAGAG67850.032.972658
GCAGAGT80350.027.4293129
GTACATG54050.023.672151
ACATGGG50800.023.5624073
GAGTACT49350.022.52223612-13
TACATGG57400.022.342652
CGCACGT550.00287905721.59037256-57
CAGAGTA79350.021.42983810-11
GTCGTAA2005.3209624E-721.4039341
AGAGTAC71600.021.3943710-11
CATGGGG29900.020.0162264
GTACTTT59000.018.67750214-15
Produced by FastQC (version 0.11.5)