FastQCFastQC Report
Fri 10 Feb 2017
SRR2031555_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031555_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3443659
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.10625326142919493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.046.367251
GTATCAA60350.041.4788741
TCAACGC63850.037.79174
CAACGCA66600.036.5165145
ATCAACG67350.035.8982933
AACGCAG68500.035.503656
ACGCAGA75800.032.0844357
CGCAGAG75550.032.0648578
TATCAAC77250.031.605182
GTACATG74850.025.003341
GCAGAGT95000.024.99
ACATGGG68250.024.7765563
TACATGG74650.023.8613532
CGCCGTA8050.022.12732994-95
CATGGGG42100.022.0011884
AGAGTAC84050.021.56008310-11
TCGCCGG3250.020.46153894-95
GAGTACT56650.019.2431612-13
CAGAGTA97000.018.63273210-11
ATAGTCG1300.007566116818.269238
Produced by FastQC (version 0.11.5)