FastQCFastQC Report
Fri 10 Feb 2017
SRR2031555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3443659
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT80700.2343437605175193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.11566185850573475No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT35750.10381399552046239TruSeq Adapter, Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.046.4127771
GTATCAA61000.037.4957161
TCAACGC66000.033.753274
CAACGCA67900.032.738825
ATCAACG68400.032.5689433
AACGCAG70600.031.6886026
TATCAAC78550.028.8442382
ACGCAGA80650.027.7398077
CGCAGAG80600.027.1676948
ACATGGG63500.023.9366383
GTACATG74050.023.8882471
TACATGG70950.023.4316332
GCAGAGT99850.021.7873469
AGAGTAC90850.019.91989110-11
CATGGGG38800.019.3424844
GAGTACT66500.017.85686712-13
TATACCG2152.3969955E-517.6741475
CAGAGTA103300.017.28918310-11
GTATTAT24850.017.2219581
TATTACG1700.00178872616.7644462
Produced by FastQC (version 0.11.5)