FastQCFastQC Report
Fri 10 Feb 2017
SRR2031553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241394
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC59070.13927024935669735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.038.1862751
GTATCAA56350.036.9210321
TCAACGC52000.036.81254
ATCAACG53200.036.071433
CAACGCA55550.034.5454565
AACGCAG55450.034.522096
CGCAGAG61700.030.5632088
ACGCAGA62850.030.3062867
TATCAAC65850.030.151862
GTACATG83600.029.4318181
ACATGGG79350.027.416513
TCGCCGG6200.024.89919394-95
GCAGAGT79400.022.9124689
TACATGG104650.022.5585292
CGCCGTA15750.021.71428594-95
CATGGGG41600.020.6670684
TCGCCGT19050.020.19685294-95
GCCGTAT13100.019.58015394-95
TAATACT52500.019.0904774
AGAGTAC73150.018.701310-11
Produced by FastQC (version 0.11.5)