FastQCFastQC Report
Fri 10 Feb 2017
SRR2031553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241394
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT250510.590631287732288No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT81640.19248388619402018TruSeq Adapter, Index 3 (95% over 21bp)
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC67390.15888644158029178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.041.1116331
GTATCAA55150.036.5673751
TCAACGC54150.034.911534
CAACGCA56650.033.370865
ATCAACG56700.033.341433
AACGCAG58300.032.5078746
CGCAGAG66250.028.7503228
ACGCAGA66600.028.5279127
TATCAAC68850.028.0785522
GTACATG79300.026.9306321
ACATGGG73050.025.228753
GCAGAGT86600.021.6103869
CATGGGG36500.020.9516034
TAATACT53950.020.6900084
TACATGG100850.019.7814272
TTAATAC61550.019.2928413
AGAGTAC78150.018.96312110-11
ATACTTC51450.018.464136
ACTTAAT46100.018.261891
CTTAATA53750.017.8507772
Produced by FastQC (version 0.11.5)