FastQCFastQC Report
Fri 10 Feb 2017
SRR2031552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265805
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.1678773586768894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.11668463941918385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.048.775171
GTATCAA28750.042.79131
TCAACGC30900.037.9692544
AACGCAG31200.037.7564136
CAACGCA31300.037.4840285
ATCAACG32000.036.9609383
TATCAAC35650.033.8429152
ACGCAGA35050.033.473617
CGCAGAG35500.032.647898
GTACATG32750.028.1374051
ACCGAAC700.00839188827.1428558
GCAGAGT42350.026.9185359
ACATGGG31350.026.6666683
TACATGG34200.025.4166682
CATGGGG18300.023.6202184
AGAGTAC37400.022.86096210-11
CAGAGTA41400.021.28321310-11
GAGTACT26550.021.20056512-13
GTATAAT15150.021.0066011
GTCGAAG1604.9897993E-520.781251
Produced by FastQC (version 0.11.5)