##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031552_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265805 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2606325618875 34.0 31.0 34.0 30.0 34.0 2 32.41012004218659 34.0 31.0 34.0 31.0 34.0 3 32.50590335794218 34.0 31.0 34.0 31.0 34.0 4 35.74752825277195 37.0 35.0 37.0 35.0 37.0 5 35.748391734903876 37.0 35.0 37.0 35.0 37.0 6 35.86548481006158 37.0 37.0 37.0 35.0 37.0 7 35.89629445293706 37.0 37.0 37.0 35.0 37.0 8 35.93400326274584 37.0 37.0 37.0 35.0 37.0 9 37.754636772646656 39.0 39.0 39.0 35.0 39.0 10-11 37.75231769506362 39.0 39.0 39.0 35.0 39.0 12-13 37.73273489992535 39.0 39.0 39.0 35.0 39.0 14-15 39.260100884417426 41.0 40.0 41.0 36.0 41.0 16-17 39.279453786325696 41.0 40.0 41.0 36.0 41.0 18-19 39.243911581957725 41.0 40.0 41.0 36.0 41.0 20-21 39.22119560279822 41.0 40.0 41.0 36.0 41.0 22-23 39.15837076010918 41.0 39.5 41.0 36.0 41.0 24-25 39.114160948961334 41.0 39.0 41.0 36.0 41.0 26-27 39.022599452522314 41.0 39.0 41.0 36.0 41.0 28-29 38.93391399149158 41.0 39.0 41.0 35.5 41.0 30-31 38.788390391885 41.0 39.0 41.0 35.0 41.0 32-33 38.699855427968764 40.5 39.0 41.0 35.0 41.0 34-35 38.59690987158369 40.0 38.5 41.0 35.0 41.0 36-37 38.46934993936665 40.0 38.0 41.0 34.0 41.0 38-39 38.3422655938316 40.0 38.0 41.0 34.0 41.0 40-41 38.239409703706336 40.0 38.0 41.0 34.0 41.0 42-43 38.119306291253395 40.0 38.0 41.0 33.0 41.0 44-45 38.269747314949775 40.0 38.0 41.0 34.0 41.0 46-47 38.17993371806874 40.0 38.0 41.0 33.0 41.0 48-49 38.07961494859003 40.0 38.0 41.0 33.0 41.0 50-51 37.07693878598994 39.0 37.0 40.0 31.5 40.5 52-53 37.166814793747854 39.5 37.5 40.0 32.5 40.5 54-55 37.979234953251094 40.0 38.0 41.0 33.0 41.0 56-57 38.058687949565694 41.0 38.0 41.0 33.0 41.0 58-59 37.91342860867195 40.0 38.0 41.0 33.0 41.0 60-61 37.80352305449891 40.0 37.5 41.0 33.0 41.0 62-63 37.60990752920078 40.0 37.0 41.0 32.5 41.0 64-65 37.402291427194555 40.0 37.0 41.0 32.0 41.0 66-67 37.17943443105376 39.5 36.0 41.0 32.0 41.0 68-69 36.93849210581409 39.0 36.0 41.0 31.5 41.0 70-71 36.62417196961617 39.0 35.0 41.0 31.0 41.0 72-73 36.30227246692816 38.5 35.0 40.5 31.0 41.0 74-75 35.933001133665925 37.0 35.0 40.0 31.0 41.0 76-77 35.54297107374359 37.0 35.0 39.0 30.5 41.0 78-79 35.107272052172334 36.5 35.0 39.0 30.0 40.5 80-81 34.695783710761134 36.0 35.0 38.5 29.5 39.5 82-83 34.321301859291125 35.5 35.0 37.0 29.0 39.0 84-85 33.939572840998416 35.0 34.0 37.0 29.0 39.0 86-87 33.61155312232137 35.0 34.0 36.5 29.0 37.0 88-89 33.32517133365724 35.0 34.0 36.0 29.0 37.0 90-91 33.084952658584854 35.0 34.0 36.0 28.0 37.0 92-93 32.88591449709868 35.0 34.0 35.5 27.0 36.0 94-95 32.71515517793025 35.0 34.0 35.0 27.0 36.0 96-97 32.56261983480868 35.0 34.0 35.0 26.5 36.0 98-99 32.41471790678659 35.0 34.0 35.0 26.0 36.0 100-101 30.940413017802904 34.0 31.5 35.0 21.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.001819689061967722 1101 2 0.04318663438190384 1101 3 0.17231539743025337 1101 4 -0.018342425931173523 1101 5 -0.09604633121485762 1101 6 -0.13712372313519694 1101 7 -0.10244062103997464 1101 8 -0.0757074836290883 1101 9 0.2909520834602404 1101 10-11 0.3905241429233328 1101 12-13 0.4458567504103712 1101 14-15 0.5088351241853601 1101 16-17 0.46265216553197064 1101 18-19 0.487134282023149 1101 20-21 0.46924251608130163 1101 22-23 0.3611704773900115 1101 24-25 0.44388987466563634 1101 26-27 0.36304684147086874 1101 28-29 0.4542175544264566 1101 30-31 0.5013008474484266 1101 32-33 0.49696711039987207 1101 34-35 0.4482821527175034 1101 36-37 0.569918779692486 1101 38-39 0.703200977106853 1101 40-41 0.4199197229319509 1101 42-43 0.5746160478888598 1101 44-45 0.6502485526504671 1101 46-47 0.6712595150699485 1101 48-49 0.5416760374483047 1101 50-51 0.6052022890095827 1101 52-53 0.669934990491619 1101 54-55 0.6272532201878107 1101 56-57 0.6170883380899284 1101 58-59 0.6384332838372799 1101 60-61 0.6143688698483984 1101 62-63 0.514569311141031 1101 64-65 0.5883311697567635 1101 66-67 0.6121925973337028 1101 68-69 0.5457957617147642 1101 70-71 0.426187566244856 1101 72-73 0.5510515329634487 1101 74-75 0.3968878331148531 1101 76-77 0.4295366580970281 1101 78-79 0.4758830052958558 1101 80-81 0.5059333513058633 1101 82-83 0.42323797066002555 1101 84-85 0.582483551226602 1101 86-87 0.4555916591982623 1101 88-89 0.5123535726706621 1101 90-91 0.4700419217903544 1101 92-93 0.4013038002841256 1101 94-95 0.4775385827327625 1101 96-97 0.6134448905387089 1101 98-99 0.496035764509017 1101 100-101 0.6091351627710004 1102 1 0.2741190762746797 1102 2 0.344421959020238 1102 3 0.41699971183398077 1102 4 0.13375994043909856 1102 5 0.21600730379847022 1102 6 0.19887593096373024 1102 7 0.2582108429354406 1102 8 0.3808526828103638 1102 9 0.5497166062184959 1102 10-11 0.6515560980782737 1102 12-13 0.6527246670685614 1102 14-15 0.7222427329915959 1102 16-17 0.7011274285185181 1102 18-19 0.8050230900887456 1102 20-21 0.8509354474911461 1102 22-23 0.8428510733572168 1102 24-25 0.8422388328930026 1102 26-27 0.8810515009617887 1102 28-29 0.7858177792621532 1102 30-31 0.8364974260798803 1102 32-33 0.8897123440865684 1102 34-35 0.7721600089447378 1102 36-37 0.7865577657954006 1102 38-39 0.8073763336901649 1102 40-41 0.8296115657707759 1102 42-43 0.7630888946546932 1102 44-45 0.83898527615343 1102 46-47 0.9674767307697891 1102 48-49 0.9439507185071676 1102 50-51 0.872488440758417 1102 52-53 0.7886089431235206 1102 54-55 0.7951335078961179 1102 56-57 0.7714492349910529 1102 58-59 0.7794388386015569 1102 60-61 0.8101666804980852 1102 62-63 0.7968932576687919 1102 64-65 0.79042005615878 1102 66-67 0.881392650236215 1102 68-69 0.8376078255233352 1102 70-71 0.7251376547548318 1102 72-73 0.6977987911887809 1102 74-75 0.6417266584755055 1102 76-77 0.721136348820778 1102 78-79 0.7758224726082119 1102 80-81 0.7116363952352955 1102 82-83 0.6757654647084976 1102 84-85 0.8281457991766317 1102 86-87 0.7162643604821639 1102 88-89 0.7763295020254546 1102 90-91 0.6112573062492217 1102 92-93 0.5410179486553446 1102 94-95 0.5924928271423937 1102 96-97 0.6193233012250161 1102 98-99 0.697551116412491 1102 100-101 0.6858717896251285 1103 1 0.27465477905227687 1103 2 0.31429774042128145 1103 3 0.3692295242977295 1103 4 0.22640832608065864 1103 5 0.3109837812103393 1103 6 0.25131035772147925 1103 7 0.2657952664709171 1103 8 0.2585714751026913 1103 9 0.3697204729453105 1103 10-11 0.4052254607128063 1103 12-13 0.4052823457435579 1103 14-15 0.4893187625076081 1103 16-17 0.4471297968886958 1103 18-19 0.464406749945681 1103 20-21 0.4240447791918669 1103 22-23 0.4250532548355963 1103 24-25 0.4946946072050409 1103 26-27 0.4066121763500661 1103 28-29 0.3557753258239913 1103 30-31 0.38533297741067685 1103 32-33 0.4259790193459594 1103 34-35 0.4628379750464333 1103 36-37 0.39818734947781564 1103 38-39 0.5226422517155598 1103 40-41 0.41346314790649785 1103 42-43 0.3333263761266281 1103 44-45 0.4638140929350243 1103 46-47 0.4816829239772389 1103 48-49 0.4396066926001012 1103 50-51 0.47920607303269236 1103 52-53 0.5561763691723698 1103 54-55 0.5089191945623526 1103 56-57 0.6049060887643378 1103 58-59 0.615747403401457 1103 60-61 0.452921401127071 1103 62-63 0.445319177627546 1103 64-65 0.47738032554074294 1103 66-67 0.41292258514631186 1103 68-69 0.4218581638619341 1103 70-71 0.5918886375479033 1103 72-73 0.4525556707402387 1103 74-75 0.4487024753215465 1103 76-77 0.29474110489476146 1103 78-79 0.3715239612980312 1103 80-81 0.3896145804515143 1103 82-83 0.4362036131907985 1103 84-85 0.4066685484193968 1103 86-87 0.49649128901216244 1103 88-89 0.534771855573176 1103 90-91 0.43149075988072383 1103 92-93 0.460084536913385 1103 94-95 0.541977666350995 1103 96-97 0.5994841731719092 1103 98-99 0.523443685852456 1103 100-101 0.6069019500942936 1104 1 0.23991660941782555 1104 2 0.2089555522100639 1104 3 0.2858077790813809 1104 4 0.17794635060554498 1104 5 0.24158359024067266 1104 6 0.1257760362284941 1104 7 0.18534073812835317 1104 8 0.2636588659710526 1104 9 0.2978210776825918 1104 10-11 0.3531066556764131 1104 12-13 0.45223247801914823 1104 14-15 0.5117298914532071 1104 16-17 0.4949624793964844 1104 18-19 0.449388850817364 1104 20-21 0.5105650757082074 1104 22-23 0.42864805347895896 1104 24-25 0.4963587470030575 1104 26-27 0.48727547081639955 1104 28-29 0.48963334090306176 1104 30-31 0.45663131446871574 1104 32-33 0.4944851094987044 1104 34-35 0.4593501262615547 1104 36-37 0.34981435628495916 1104 38-39 0.504094151658407 1104 40-41 0.3715439369739926 1104 42-43 0.41881781500435267 1104 44-45 0.44067399375400385 1104 46-47 0.5662682836538906 1104 48-49 0.7547845031568201 1104 50-51 0.6006202581774431 1104 52-53 0.40743178939119673 1104 54-55 0.5112361562965475 1104 56-57 0.5810096401841207 1104 58-59 0.6374915930295444 1104 60-61 0.5440127345950145 1104 62-63 0.6220679361535915 1104 64-65 0.683417088912897 1104 66-67 0.6768872420682754 1104 68-69 0.5994834834281093 1104 70-71 0.45630060155798446 1104 72-73 0.4996140362726109 1104 74-75 0.5097237865781494 1104 76-77 0.5786360818449268 1104 78-79 0.5076433241848974 1104 80-81 0.4457007974382279 1104 82-83 0.4872075729023564 1104 84-85 0.37903997757751995 1104 86-87 0.3665951244907646 1104 88-89 0.41157374678891756 1104 90-91 0.5429162560425098 1104 92-93 0.5449124588690779 1104 94-95 0.4981650151239023 1104 96-97 0.40440249770397685 1104 98-99 0.5503539814447507 1104 100-101 0.35828288666703756 1105 1 0.13973983712318017 1105 2 0.12179010743339092 1105 3 0.2322211539281014 1105 4 0.17081291958434264 1105 5 0.1189409829720276 1105 6 0.09634063886289823 1105 7 0.07458850195486377 1105 8 0.12732254104568597 1105 9 0.3779219637606417 1105 10-11 0.28973659835405385 1105 12-13 0.33643831285064607 1105 14-15 0.3879651403079052 1105 16-17 0.3516969655100084 1105 18-19 0.3108996720841901 1105 20-21 0.34113322487140607 1105 22-23 0.42356218869195317 1105 24-25 0.44225439197652605 1105 26-27 0.4047553454529407 1105 28-29 0.3367225353106349 1105 30-31 0.35301567586000004 1105 32-33 0.4051968930781129 1105 34-35 0.4205116777729003 1105 36-37 0.42581575589721155 1105 38-39 0.37698553050605454 1105 40-41 0.3927479043364315 1105 42-43 0.2797566793393358 1105 44-45 0.3084284472996188 1105 46-47 0.23064791422908826 1105 48-49 0.31152381824927744 1105 50-51 0.3280164806492962 1105 52-53 0.27227143958312894 1105 54-55 0.31748920904245637 1105 56-57 0.418239405376994 1105 58-59 0.3937971708168746 1105 60-61 0.4704923218677237 1105 62-63 0.4424908755451469 1105 64-65 0.3175273337104656 1105 66-67 0.42501957509050214 1105 68-69 0.41529237302419375 1105 70-71 0.4921427081960985 1105 72-73 0.4344133209504122 1105 74-75 0.3696220455333332 1105 76-77 0.24906816463104064 1105 78-79 0.3101586680675652 1105 80-81 0.26050589272348645 1105 82-83 0.2777217166650203 1105 84-85 0.22404994031522563 1105 86-87 0.19645634280257696 1105 88-89 0.27898106799597144 1105 90-91 0.30610827445278943 1105 92-93 0.3154536290810839 1105 94-95 0.28700326570357504 1105 96-97 0.08491210539298066 1105 98-99 0.034695027686588276 1105 100-101 0.14954552216541472 1106 1 0.37899195763061044 1106 2 0.3484473827490504 1106 3 0.3068302203085551 1106 4 0.4214718048458792 1106 5 0.297363236001857 1106 6 0.32302847333661333 1106 7 0.32706677513883164 1106 8 0.15267471670867394 1106 9 0.30903864011680326 1106 10-11 0.2754967760443776 1106 12-13 0.2368348365600852 1106 14-15 0.3142978177373621 1106 16-17 0.32453844546767385 1106 18-19 0.3739849544955334 1106 20-21 0.3939439220674146 1106 22-23 0.3715587004758589 1106 24-25 0.3145905278082566 1106 26-27 0.4187633653685623 1106 28-29 0.3471525250248746 1106 30-31 0.3287643752324243 1106 32-33 0.1884411576458902 1106 34-35 0.13910916148711294 1106 36-37 0.2110281047784568 1106 38-39 0.42888057097830057 1106 40-41 0.3550352945843329 1106 42-43 0.3164787251631651 1106 44-45 0.37002341174664366 1106 46-47 0.43929876466809503 1106 48-49 0.3563447863037794 1106 50-51 0.2998189492329857 1106 52-53 0.351003010920131 1106 54-55 0.33517588077747007 1106 56-57 0.4299238112622419 1106 58-59 0.4002015504659724 1106 60-61 0.3271158330404518 1106 62-63 0.270622071228118 1106 64-65 0.3211403951418319 1106 66-67 0.2852062095582468 1106 68-69 0.20053155433689795 1106 70-71 0.29166307848365136 1106 72-73 0.27851913017182284 1106 74-75 0.3193749635602501 1106 76-77 0.1766448233970479 1106 78-79 0.3648055234556651 1106 80-81 0.236424530828522 1106 82-83 0.3432442782678109 1106 84-85 0.34657800256646 1106 86-87 0.16954825878725188 1106 88-89 0.16404136643222955 1106 90-91 0.24960476387634145 1106 92-93 0.3002340503502552 1106 94-95 0.46696316612544564 1106 96-97 0.3730309283436526 1106 98-99 0.39077145539553726 1106 100-101 0.5566345014895369 1107 1 0.10624917529560918 1107 2 0.136034948728188 1107 3 0.18030347351524512 1107 4 0.18174714153097682 1107 5 0.14806446018469188 1107 6 0.1324250166565335 1107 7 0.20101354616511458 1107 8 0.1892790368624233 1107 9 0.20913891447859356 1107 10-11 0.26431688105391515 1107 12-13 0.277915292808423 1107 14-15 0.3818273777836012 1107 16-17 0.21373353480934298 1107 18-19 0.3065093615537293 1107 20-21 0.33661066301139186 1107 22-23 0.2536604232451012 1107 24-25 0.30374244346637624 1107 26-27 0.24574552488836332 1107 28-29 0.23702237725945707 1107 30-31 0.28331850249730195 1107 32-33 0.31936361213943343 1107 34-35 0.3587911385632765 1107 36-37 0.36409413266029844 1107 38-39 0.3299463342472464 1107 40-41 0.39583268466553534 1107 42-43 0.37634495637913545 1107 44-45 0.28573758803455007 1107 46-47 0.32340632284811477 1107 48-49 0.33999832445677924 1107 50-51 0.3078210522091851 1107 52-53 0.2833310473531512 1107 54-55 0.3137843651026415 1107 56-57 0.2751694834486429 1107 58-59 0.16124043463515392 1107 60-61 0.25212767098594213 1107 62-63 0.3025563218861649 1107 64-65 0.27305222333841783 1107 66-67 0.4280219693454441 1107 68-69 0.2868900630342992 1107 70-71 0.17954067412728136 1107 72-73 0.028527486368638222 1107 74-75 0.013220972779919293 1107 76-77 0.0808741035106948 1107 78-79 0.046665961041838955 1107 80-81 0.05855522174672245 1107 82-83 0.006559877894567023 1107 84-85 0.08516761581381616 1107 86-87 0.07664928520676284 1107 88-89 -0.03594331387360228 1107 90-91 0.14121688314713055 1107 92-93 0.2474016478044092 1107 94-95 0.234887208296378 1107 96-97 0.13028515170479693 1107 98-99 0.1339691630122175 1107 100-101 0.17379882609992947 1108 1 0.1909547179981601 1108 2 0.20679261731562093 1108 3 0.1856666838907799 1108 4 0.11358061355488758 1108 5 0.20142941584072105 1108 6 0.22224164463353446 1108 7 0.18729689558829676 1108 8 0.26524511362602965 1108 9 0.2973924974636759 1108 10-11 0.25673549232796233 1108 12-13 0.3642808768176877 1108 14-15 0.37410555668268586 1108 16-17 0.3234370200143033 1108 18-19 0.3266836152699639 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86-87 0.7438934664076342 1108 88-89 0.8182813491866767 1108 90-91 0.8121057743584075 1108 92-93 0.7806798750383592 1108 94-95 0.8271865500277897 1108 96-97 0.7471686503128794 1108 98-99 0.7826909671306055 1108 100-101 0.7279583677286574 1109 1 0.10176238303628082 1109 2 0.10806738120118098 1109 3 0.18214946804479837 1109 4 0.2532456613585339 1109 5 0.15598872938472397 1109 6 0.07428869571361929 1109 7 0.11696951744463746 1109 8 0.12746510960911905 1109 9 0.11377253584210223 1109 10-11 0.17803914892573403 1109 12-13 0.1984399754812145 1109 14-15 0.1799254781351607 1109 16-17 0.19326313137948858 1109 18-19 0.2775024840984983 1109 20-21 0.20760772517863302 1109 22-23 0.1786956609794359 1109 24-25 0.13048003584426482 1109 26-27 0.09705322941417194 1109 28-29 0.17197452022647752 1109 30-31 0.20716903947409548 1109 32-33 0.25718261212567484 1109 34-35 0.2217801363858527 1109 36-37 0.2137781499246003 1109 38-39 0.3099843297173024 1109 40-41 0.1335773320943332 1109 42-43 0.25686840088486207 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-0.6457320981221919 2316 98-99 -0.5492242188346701 2316 100-101 -0.6067911149326584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 28.0 7 376.0 8 942.0 9 795.0 10 809.0 11 1051.0 12 1334.0 13 1604.0 14 1709.0 15 1818.0 16 2164.0 17 2532.0 18 3004.0 19 3944.0 20 5154.0 21 5999.0 22 6816.0 23 7030.0 24 6740.0 25 6498.0 26 6625.0 27 7420.0 28 8587.0 29 10087.0 30 12355.0 31 15045.0 32 19117.0 33 25496.0 34 36670.0 35 56332.0 36 101145.0 37 220931.0 38 448987.0 39 236356.0 40 305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.41104277515099 22.65269927042475 14.759935377092049 22.17632257733221 2 12.88215799431982 22.981501890101555 43.75136770671628 20.384972408862346 3 15.708659706668879 30.383273884998086 33.004293710326635 20.903772698006406 4 10.388487958255814 19.867989145247492 44.95234258041326 24.79118031608344 5 10.80087375227622 40.33077764742595 37.873290119726185 10.995058480571652 6 24.23185245752703 43.42999119137624 21.018403308566487 11.319753042530248 7 22.419408992696347 35.37353699819483 26.927133326223235 15.279920682885596 8 22.306595407665476 38.209676845959684 23.507491280252488 15.976236466122348 9 24.850984156327396 17.661251140578525 23.109957694905614 34.37780700818847 10-11 21.54640722702154 28.263713605176154 32.599926528967735 17.589952638834575 12-13 22.654042289294164 26.42057820912384 31.86422869241313 19.06115080916887 14-15 20.268208768333196 27.89059136280865 29.178427956912795 22.662771911945363 16-17 19.425938434435004 31.280133985882504 29.39923605926663 19.89469152041586 18-19 19.498342951718474 30.283574484221504 31.174232997973622 19.043849566086404 20-21 20.557471332472222 29.54665213046243 30.773144362678295 19.12273217438705 22-23 20.257741121262754 29.28049739098834 30.655551210494508 19.806210277254397 24-25 19.85203092103444 29.468480532151474 30.785468535832926 19.894020010981155 26-27 19.991033374018905 29.653698634465815 30.5664774590083 19.78879053250698 28-29 19.992494894553268 29.430718001587923 30.656222719929215 19.920564383929594 30-31 20.38023234226441 29.567626925158297 30.434545605365752 19.617595127211537 32-33 20.043055604931247 29.7641816867527 30.46610654879701 19.72665615951904 34-35 20.11226057726111 29.714134483589493 30.435059112580532 19.738545826568863 36-37 20.369606692974035 29.286580476455693 30.397257081461994 19.946555749108274 38-39 20.135091898041168 29.658201697733855 30.441379201377778 19.7653272028472 40-41 20.0884022420515 29.556882774202975 30.637973463527164 19.71674152021836 42-43 20.094564328628817 29.5749740283851 30.195448746054883 20.1350128969312 44-45 20.1265202776099 29.851319911044747 30.01627422865291 20.005885582692436 46-47 20.171898786778037 29.56032652790363 30.069884492294612 20.197890193023717 48-49 20.032406408916422 29.48611904417684 30.170982912768928 20.310491634137804 50-51 19.944936226353978 29.69177716946923 29.935693096488002 20.427593507688783 52-53 20.112221076706128 29.736017791049964 29.83441367351211 20.317347458731795 54-55 20.12999632644839 29.65634517164966 29.887620921073943 20.32603758082801 56-57 20.16392730317861 29.686918601206347 29.68593108733178 20.463223008283265 58-59 20.05822381804464 29.515051686476195 29.779389400421074 20.647335095058086 60-61 20.105071476254242 29.628181275946925 29.847843862206265 20.418903385592568 62-63 19.848910377190798 29.546059622137694 30.064622907951854 20.540407092719654 64-65 19.959353928922702 29.371704172443625 29.995970943391754 20.672970955241922 66-67 20.07102199785907 29.343342773965976 29.821101986482912 20.764533241692046 68-69 20.019276270831604 29.404766136964223 29.791871575795643 20.78408601640853 70-71 20.136197913580688 29.051117668203236 29.872294705740615 20.94038971247546 72-73 20.134973396376218 29.038082485058915 29.69146116502937 21.135482953535497 74-75 19.968992064338504 29.131106292043402 29.75513605966164 21.144765583956453 76-77 20.022910321890024 29.2027602987822 29.8119378577269 20.96239152160088 78-79 20.16914137643634 29.304158223423038 29.42842696939892 21.098273430741703 80-81 20.342904317805665 29.199995259933402 29.29226855637322 21.164831865887717 82-83 20.318334972606365 29.018292707012534 29.495972918419504 21.1673994019616 84-85 20.400061620865774 28.84836131947654 29.50209550444184 21.24948155521585 86-87 20.048862186513723 29.142403450768484 29.577976070563793 21.230758292154007 88-89 20.46780507266127 28.632885792045375 29.526072341316397 21.373236793976954 90-91 20.50039303052208 28.61696706838731 29.71974356239705 21.16289633869356 92-93 20.67719751462508 28.54634007607807 29.558936803062082 21.21752560623477 94-95 20.623081754298646 28.39528995382385 29.611117036194358 21.37051125568314 96-97 20.585240222625128 28.20098672386347 29.587258700984748 21.62651435252665 98-99 20.706348924202388 28.241435292165857 29.508257591019156 21.543958192612607 100-101 21.004538613767522 28.105553383025033 29.42578043221507 21.46412757099237 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 9.5 2 23.5 3 51.5 4 48.0 5 48.0 6 80.5 7 131.5 8 266.0 9 346.5 10 333.5 11 344.0 12 387.0 13 455.5 14 516.5 15 603.5 16 686.0 17 772.0 18 872.5 19 970.5 20 1077.5 21 1264.0 22 1549.5 23 1861.5 24 2338.5 25 3106.5 26 4331.5 27 6472.5 28 10126.0 29 16249.5 30 24715.5 31 33782.5 32 43499.5 33 53626.0 34 62506.0 35 68136.5 36 73919.0 37 77536.5 38 73554.5 39 68064.0 40 62809.5 41 56375.5 42 49731.0 43 43915.0 44 39357.5 45 35728.5 46 32531.0 47 29629.5 48 27947.5 49 26824.5 50 25368.0 51 23746.0 52 22394.5 53 21362.5 54 20182.5 55 18579.0 56 16736.5 57 14670.0 58 12732.5 59 10878.0 60 8871.0 61 7140.0 62 5700.5 63 4411.5 64 3258.0 65 2416.0 66 1855.0 67 1385.0 68 1030.0 69 808.5 70 626.0 71 496.5 72 385.0 73 286.0 74 239.5 75 179.5 76 132.5 77 97.5 78 63.5 79 50.0 80 40.0 81 28.0 82 24.0 83 23.0 84 20.5 85 15.0 86 12.0 87 10.0 88 9.0 89 9.0 90 5.0 91 2.5 92 2.0 93 1.5 94 1.5 95 2.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.5800221993119004E-4 48-49 4.7400665979357005E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1265805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.75996477504376 #Duplication Level Percentage of deduplicated Percentage of total 1 70.39140831475903 35.73065406522891 2 14.969911155180979 15.197443258450422 3 5.72858991776857 8.723490673098103 4 2.822465228977805 5.730729424067968 5 1.6399279962665607 4.162134366204936 6 1.045825715965409 3.185164590194345 7 0.7106667432972471 2.525139319959433 8 0.5064065355060386 2.0564142323310777 9 0.3797644423916553 1.7349146746753104 >10 1.690382998895748 14.402651129416 >50 0.07789120392981164 2.6890491787492956 >100 0.03534591619095457 3.148781256191243 >500 0.0010996462324473898 0.4262103307264128 >1k 3.141846378421114E-4 0.28722350070655545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.1678773586768894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1477 0.11668463941918385 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.900110996559502E-5 0.0 16-17 0.0 0.0 0.0 9.085127646043427E-4 0.0 18-19 0.0 0.0 0.0 0.0018170255292086854 0.0 20-21 0.0 0.0 0.0 0.0030810432886582057 0.0 22-23 0.0 0.0 0.0 0.006280588242264804 0.0 24-25 0.0 0.0 0.0 0.009480133195871402 0.0 26-27 0.0 0.0 0.0 0.011178657060131695 0.0 28-29 0.0 0.0 0.0 0.014931209783497459 0.0 30-31 0.0 0.0 0.0 0.025675360738818383 0.0 32-33 0.0 0.0 0.0 0.05265423979206908 0.0 34-35 0.0 0.0 0.0 0.08666421763225773 0.0 36-37 0.0 0.0 0.0 0.1444140290171077 0.0 38-39 0.0 0.0 0.0 0.25102602691567816 0.0 40-41 0.0 0.0 0.0 0.393860033733474 0.0 42-43 0.0 0.0 0.0 0.5389455721852892 0.0 44-45 0.0 0.0 0.0 0.705756415877643 0.0 46-47 0.0 0.0 0.0 0.9192174150046808 0.0 48-49 0.0 0.0 0.0 1.1442520767416782 0.0 50-51 0.0 0.0 0.0 1.3974111336264274 0.0 52-53 0.0 0.0 0.0 1.6534932315799038 0.0 54-55 0.0 0.0 0.0 1.905072266265341 0.0 56-57 0.0 0.0 0.0 2.2030644530555654 0.0 58-59 0.0 0.0 0.0 2.5403201914986906 0.0 60-61 0.0 0.0 0.0 2.9172739876995273 0.0 62-63 0.0 0.0 0.0 3.296755819419263 0.0 64-65 0.0 0.0 0.0 3.6518658087146125 0.0 66-67 0.0 0.0 0.0 4.0444618246886375 0.0 68-69 0.0 0.0 0.0 4.504959294678089 0.0 70-71 0.0 0.0 0.0 5.013726442856522 0.0 72-73 0.0 0.0 0.0 5.528418674282374 0.0 74-75 0.0 0.0 0.0 6.022412614897239 0.0 76-77 0.0 0.0 0.0 6.514747532202827 0.0 78-79 0.0 0.0 0.0 7.066925790307353 0.0 80-81 0.0 0.0 0.0 7.68321344914896 0.0 82-83 0.0 0.0 0.0 8.33738214021907 0.0 84-85 0.0 0.0 0.0 8.941661630345907 0.0 86-87 0.0 0.0 0.0 9.543571087173774 0.0 88-89 0.0 0.0 0.0 10.216542042415696 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1490 0.0 48.77517 1 GTATCAA 2875 0.0 42.7913 1 TCAACGC 3090 0.0 37.969254 4 AACGCAG 3120 0.0 37.756413 6 CAACGCA 3130 0.0 37.484028 5 ATCAACG 3200 0.0 36.960938 3 TATCAAC 3565 0.0 33.842915 2 ACGCAGA 3505 0.0 33.47361 7 CGCAGAG 3550 0.0 32.64789 8 GTACATG 3275 0.0 28.137405 1 ACCGAAC 70 0.008391888 27.142855 8 GCAGAGT 4235 0.0 26.918535 9 ACATGGG 3135 0.0 26.666668 3 TACATGG 3420 0.0 25.416668 2 CATGGGG 1830 0.0 23.620218 4 AGAGTAC 3740 0.0 22.860962 10-11 CAGAGTA 4140 0.0 21.283213 10-11 GAGTACT 2655 0.0 21.200565 12-13 GTATAAT 1515 0.0 21.006601 1 GTCGAAG 160 4.9897993E-5 20.78125 1 >>END_MODULE