FastQCFastQC Report
Fri 10 Feb 2017
SRR2031552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265805
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT30980.2447454386734134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.19750277491398754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.1413329857284495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.051.1911661
GTATCAA30400.043.176961
TCAACGC33150.038.543684
ATCAACG33450.038.3399963
AACGCAG33800.038.083516
CAACGCA33700.037.9146275
ACGCAGA38050.033.7049337
CGCAGAG37950.033.4182628
TATCAAC39350.032.953562
TATACCG902.4135046E-531.6659975
GCAGAGT45550.027.5296049
CGTATAC1457.8061566E-726.238471
AGAGTAC41550.022.34910610-11
GTACATG34550.022.2989031
ACATGGG32000.021.2261123
GAGTACT28100.021.04492812-13
CAGAGTA45000.020.95233310-11
CATGGGG18750.020.5195664
GTATAAA13200.020.1757951
TAATACT12450.019.8389364
Produced by FastQC (version 0.11.5)