FastQCFastQC Report
Fri 10 Feb 2017
SRR2031550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073296
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.20098432640587738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28300.1364976346840972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.051.673641
GTATCAA49550.044.4803241
TCAACGC54700.039.510974
ATCAACG56550.038.4703833
AACGCAG56900.038.0667846
CAACGCA56750.038.0000045
TATCAAC61150.035.7318042
ACGCAGA64900.033.2280437
CGCAGAG66100.032.6966748
GTACATG62000.027.5806431
GCAGAGT77750.027.2475879
ACATGGG60950.026.263333
TACATGG63300.025.7385482
AGAGTAC69800.022.7632510-11
CATGGGG37550.021.8841554
CAGAGTA74350.021.78547510-11
TAGGACG1353.7973662E-421.111114
GAGTACT44800.021.0993312-13
CATGGGA24150.019.275364
TAGCGCT1300.007565125418.2692324
Produced by FastQC (version 0.11.5)