FastQCFastQC Report
Fri 10 Feb 2017
SRR2031550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073296
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.2356151750642455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.17503530610197485No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT25540.12318549787391671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.057.2362141
GTATCAA50550.044.7894481
TCAACGC56850.039.6031654
ATCAACG57850.038.8364753
CAACGCA59050.038.208135
AACGCAG59500.038.0788156
TATCAAC62950.035.539162
ACGCAGA68300.033.1726157
CGCAGAG69050.032.8123058
GCAGAGT80950.027.5780169
GTACATG53300.026.236751
ACATGGG53200.023.9279353
TACATGG56600.023.5815262
AGAGTAC73400.022.26098810-11
GAGTACT53900.022.07501612-13
CAGAGTA77900.022.0725810-11
CATGGGG30050.021.9711284
GTGGTAT12750.019.3991681
AGTACTT62450.018.7865312-13
GTACTTT63350.018.74456614-15
Produced by FastQC (version 0.11.5)