FastQCFastQC Report
Fri 10 Feb 2017
SRR2031549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618546
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC56230.34741057714763746No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC31140.1923949025854069No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT28550.1763928859606091No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA28430.17565147978494278No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT24340.15038188596431612No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT22350.13808690021784986No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT20360.12579191447138358No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT20290.12535942753557822No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA19130.11819250117080392No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA18810.11621541803569375No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT17000.10503254155272694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.044.5595251
GTATCAA34350.040.9315871
AACGCAG38600.034.7020726
TCAACGC39700.033.501264
CAACGCA40300.033.1203465
ATCAACG40400.033.0383643
CGCAGAG41550.031.4380258
ACGCAGA42550.031.2573437
GTACATG54150.030.7017561
ACATGGG51050.029.5886383
TATCAAC45650.029.5509322
TCGCCGG2200.028.06818294-95
GCAGAGT46000.027.9836989
CATGGGA35700.026.876754
AGAGTCG908.9491304E-426.3888918
ATGGGAT21600.023.755
CATGGGG15300.023.594774
CAGAGTA47800.022.75627510-11
AGACCGT1703.150304E-622.352946
TACATGG73900.021.8538572
Produced by FastQC (version 0.11.5)