FastQCFastQC Report
Fri 10 Feb 2017
SRR2031549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618546
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT300941.859323120875156No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT83600.5165129690475279TruSeq Adapter, Index 3 (95% over 21bp)
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC59110.36520432536362885No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA32070.1981408004468208No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC30150.18627830163615988No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT28880.17843175294369143No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT28770.17775213061599732RNA PCR Primer, Index 3 (95% over 23bp)
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT26700.1649628740857535No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT23650.14611880045423484No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG23540.14543917812654072TruSeq Adapter, Index 3 (96% over 28bp)
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT22120.13666587171448943No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA20020.12369126364032902No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT19380.11973709737010874No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA18810.11621541803569375No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG17510.10818351779930876No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG16490.10188156530614514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.10083123989061789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.047.9155731
GTATCAA32950.037.3957821
CAGTGCG651.3286681E-436.537329
AACGCAG38750.030.6442076
GTACATG52700.030.5129641
ACATGGG47450.030.330983
TCAACGC39700.030.2698354
GCGGATA552.7361402E-630.2263334-35
ATCAACG40000.030.0428123
CAACGCA40050.030.0053025
GGGACCG804.4952641E-429.6865755
CATGGGA33200.028.4704974
CGCAGAG42400.027.8941758
ATGGGAT20350.027.3087315
ACGCAGA43900.027.0492677
GCGTTAC458.9527044E-426.38806712-13
TATCAAC46850.026.258522
ACTTCAG24550.025.5389188
TTGCACG950.001226994424.999229
CTTAATA27500.024.871952
Produced by FastQC (version 0.11.5)