FastQCFastQC Report
Fri 10 Feb 2017
SRR2031548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966257
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.22860694202232976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32830.16696698346147018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.051.7202221
GTATCAA50550.046.889221
TCAACGC62550.037.286174
ATCAACG63400.036.6364363
CAACGCA65150.035.8710675
AACGCAG65750.035.7604566
TATCAAC66250.035.633962
ACGCAGA74450.031.2625927
CGCAGAG77450.029.6836668
GTACATG69650.028.0294341
CCGTCGA700.00839299627.1428599
TACATGG71550.026.1565342
ACATGGG70300.026.0135143
GCAGAGT87050.025.7007459
CATGGGG45250.023.1988954
AGAGTAC77200.021.81185210-11
GAGTACT44750.020.6452512-13
CAGAGTA84150.020.6030910-11
AGTACTT51100.019.79941412-13
GTACCGT2552.677225E-718.627456
Produced by FastQC (version 0.11.5)