FastQCFastQC Report
Fri 10 Feb 2017
SRR2031548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966257
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT77580.3945567644514425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56010.284855947111695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.21792675118257684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.12485651672187309No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT23300.11849926026963921RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.056.932721
GTATCAA54100.043.795651
TCAACGC68150.034.639454
CAACGCA69550.034.0787665
ATCAACG69300.033.9960863
AACGCAG70350.033.8937876
TATCAAC70650.033.6154022
ACGCAGA79500.029.8135627
CGCAGAG81950.028.864298
GTACATG62400.028.4395771
TACATGG64450.026.7524872
ACATGGG64000.025.9757773
GCAGAGT92750.024.7863129
GAGTACT52400.022.97878812-13
CATGGGG38700.022.5832984
AGAGTAC81600.021.85748910-11
CAGAGTA90000.021.26880310-11
TAGTACG1150.003689328220.685741
AGTACTT58050.020.49679612-13
GGACCGT1656.3271145E-520.1509066
Produced by FastQC (version 0.11.5)