FastQCFastQC Report
Fri 10 Feb 2017
SRR2031547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931530
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.14579116037545364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.1041661273705301No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.051.246821
GTATCAA37500.044.3333321
TCAACGC44150.036.149494
CAACGCA44500.035.4382065
ATCAACG45750.034.989073
AACGCAG46000.034.282616
TATCAAC49600.032.5604822
ACGCAGA51650.030.1645687
CGCAGAG53600.029.1557858
GTACATG56700.027.7292771
TACATGG55400.027.3510822
ACATGGG57850.025.453763
TATACCG1151.2821947E-424.7826085
CATGGGG41800.023.754
GTATACG1000.001654052123.751
GCAGAGT67400.023.0452529
AGAGTAC56350.021.031510-11
ATACGAC1604.991542E-520.781253
GAGTACT35650.019.45301612-13
CAGAGTA63750.019.40980510-11
Produced by FastQC (version 0.11.5)