FastQCFastQC Report
Fri 10 Feb 2017
SRR2031547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931530
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.17141851278520137No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT31940.165361138579261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26070.13497072269133795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.051.126591
GTATCAA38400.041.3646281
TCAACGC44200.035.1407244
CAACGCA44950.034.5543945
ATCAACG45950.033.8023953
AACGCAG47250.032.9729086
TATCAAC49700.031.347482
ACGCAGA52500.029.8565647
CGCAGAG54650.028.508148
GTACATG47450.027.8626541
TACATGG48700.026.1391262
ACATGGG49400.025.3841233
GCAGAGT65950.023.551489
CATGGGG35400.021.0659754
GAGTACT37900.020.99233812-13
AGAGTAC57600.020.82207910-11
CAGAGTA63000.019.82898110-11
TATTGCG1300.007565788518.2688777
ATTATAC11450.018.2529223
GTACTTT42450.018.2387514-15
Produced by FastQC (version 0.11.5)