FastQCFastQC Report
Fri 10 Feb 2017
SRR2031546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573281
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46200.29365383551952895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.20721028220642085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.12769492544561334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.059.3483161
GTATCAA48650.047.6464581
TCAACGC58250.038.8154534
CAACGCA59450.037.952065
ATCAACG60250.037.7634853
AACGCAG61600.036.9358756
TATCAAC63800.036.1833882
ACGCAGA68750.032.7490927
CGCAGAG68750.032.688
GTACATG64300.028.5147741
GCAGAGT78800.028.2106599
TACATGG64500.028.2054252
ACATGGG65800.026.4209733
ATACCGT1401.780023E-523.7499986
AGAGTAC70650.023.59872610-11
CATGGGA31850.023.4144424
CAGAGTA75700.022.99702810-11
GAGTACT46700.022.78372612-13
CATGGGG32700.022.6605514
CCGTCTA1050.002196579522.6190479
Produced by FastQC (version 0.11.5)