FastQCFastQC Report
Fri 10 Feb 2017
SRR2031546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573281
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55920.3554355515638974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41270.26231804744352727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26110.16595891007391558No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT25560.1624630310796355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.064.481831
GTATCAA46500.047.6800581
TCAACGC56800.038.300344
ATCAACG57850.037.605173
CAACGCA58600.037.2049335
AACGCAG60450.036.1448976
TATCAAC60950.035.9263152
ACGCAGA68100.031.945077
CGCAGAG70000.031.0101328
GCAGAGT78950.027.1337539
GTACATG53750.025.0502321
TACATGG55000.024.6131742
ACATGGG53650.024.6127723
ACCGTGC1601.860586E-623.7495568
GTACGGC1000.001654096323.7495546
AGAGTAC71100.023.31531510-11
CAGAGTA75550.022.2249310-11
TATACTG11700.021.1107165
GAGTACT53200.020.93710912-13
CATGGGG25150.020.5860964
Produced by FastQC (version 0.11.5)