FastQCFastQC Report
Fri 10 Feb 2017
SRR2031541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969905
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.15692258520164346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.11867141627272773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.052.6855851
GTATCAA22300.044.304931
TCAACGC25550.038.1115464
ATCAACG25650.037.777783
CAACGCA26450.037.1739125
AACGCAG26550.036.6760836
TATCAAC28900.033.693772
ACGCAGA29550.032.6311347
CGCAGAG29800.032.1979878
GTACATG29250.030.5299151
ACATGGG29150.027.0497423
GCAGAGT34700.026.5561949
TACATGG32250.026.511632
TAAACCG950.001226416625.05
CATGGGG15850.024.8738174
CGCCGTA2700.024.6296394-95
AGAGTAC31000.021.83467710-11
TCGCCGT4100.020.85365994-95
GAGTACT18800.020.46542512-13
CTGGTCG1656.3214124E-520.1515169
Produced by FastQC (version 0.11.5)