FastQCFastQC Report
Fri 10 Feb 2017
SRR2031541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969905
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.2107422891932715No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT19250.19847304632927965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.1712538856898356No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC9730.100319103417345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.057.075291
GTATCAA24800.045.4531061
GTACCGT652.5893332E-643.8452226
TCAACGC28000.039.8652274
ATCAACG28150.039.65283
AACGCAG28700.039.0584036
CAACGCA28500.038.9991725
TATCAAC31800.034.8027232
ACGCAGA32350.034.7983367
CGCAGAG32200.034.370398
TATACCG953.490792E-529.9993655
ATAGCGT856.407246E-427.9405846
TACCGTA856.407246E-427.9405847
GCAGAGT37800.027.6449189
GTACATG23400.027.6433051
ACATGGG22650.026.8427013
TACATGG26100.024.5684432
TAAATCG1201.7107937E-423.7494965
AGAGTAC33600.022.83061810-11
CAGAGTA36800.022.20061910-11
Produced by FastQC (version 0.11.5)